Binding Site Information of Target
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T46185 | Target Info | |||
| Target Name | Muscarinic acetylcholine receptor M2 (CHRM2) | ||||
| Synonyms | M2 receptor; CHRM2 | ||||
| Target Type | Successful Target | ||||
| Gene Name | CHRM2 | ||||
| Biochemical Class | GPCR rhodopsin | ||||
| UniProt ID | |||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| Ligand Name: Beta-D-Glucose | Ligand Info | |||||
| Structure Description | Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist | PDB:3UON | ||||
| Method | X-ray diffraction | Resolution | 3.00 Å | Mutation | Yes | [1] |
| PDB Sequence |
TFEVVFIVLV
29 AGSLSLVTII39 GNILVMVSIK49 VNRHLQTVNN59 YFLFSLACAD69 LIIGVFSMNL 79 YTLYTVIGYW89 PLGPVVCDLW99 LALDYVVSNA109 SVMNLLIISF119 DRYFCVTKPL 129 TYPVKRTTKM139 AGMMIAAAWV149 LSFILWAPAI159 LFWQFIVGVR169 TVEDGECYIQ 179 FFSNAAVTFG189 TAIAAFYLPV199 IIMTVLYWHI209 SRASKSRINI1003 FEMLRIDEGL 1013 RLKIYKDTEG1023 YYTIGIGHLL1033 TKSPSLNAAK1043 SELDKAIGRN1053 TNGVITKDEA 1063 EKLFNQDVDA1073 AVRGILRNAK1083 LKPVYDSLDA1093 VRRAALINMV1103 FQMGETGVAG 1113 FTNSLRMLQQ1123 KRWDEAAVNL1133 AKSRWYNQTP1143 NRAKRVITTF1153 RTGTWDAYPP 378 PSREKKVTRT388 ILAILLAFII398 TWAPYNVMVL408 INTFCAPCIP418 NTVWTIGYWL 428 CYINSTINPA438 CYALCNATFK448 KTFKHLLM
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| Ligand Name: L-serine-O-phosphate | Ligand Info | |||||
| Structure Description | GPCR-Beta arrestin structure in lipid bilayer | PDB:6U1N | ||||
| Method | Electron microscopy | Resolution | 4.00 Å | Mutation | Yes | [2] |
| PDB Sequence |
TFEVVFIVLV
29 AGSLSLVTII39 GNILVMVSIK49 VNRHLQTVNN59 YFLFSLACAD69 LIIGVFSMNL 79 YTLYTVIGYW89 PLGPVVCDLW99 LALDYVVSNA109 SVMNLLIISF119 DRYFCVTKPL 129 TYPVKRTTKM139 AGMMIAAAWV149 LSFILWAPAI159 LFWQFIVGVR169 TVEDGECYIQ 179 FFSNAAVTFG189 TAIAAFYLPV199 IIMTVLYWHI209 SRASEKKVTR387 TILAILLAFI 397 ITWAPYNVMV407 LINTFCAPCI417 PNTVWTIGYW427 LCYINSTINP437 ACYALCNATF 447 KKTFKECALA497 KD
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| Ligand Name: Phosphonothreonine | Ligand Info | |||||
| Structure Description | GPCR-Beta arrestin structure in lipid bilayer | PDB:6U1N | ||||
| Method | Electron microscopy | Resolution | 4.00 Å | Mutation | Yes | [2] |
| PDB Sequence |
TFEVVFIVLV
29 AGSLSLVTII39 GNILVMVSIK49 VNRHLQTVNN59 YFLFSLACAD69 LIIGVFSMNL 79 YTLYTVIGYW89 PLGPVVCDLW99 LALDYVVSNA109 SVMNLLIISF119 DRYFCVTKPL 129 TYPVKRTTKM139 AGMMIAAAWV149 LSFILWAPAI159 LFWQFIVGVR169 TVEDGECYIQ 179 FFSNAAVTFG189 TAIAAFYLPV199 IIMTVLYWHI209 SRASEKKVTR387 TILAILLAFI 397 ITWAPYNVMV407 LINTFCAPCI417 PNTVWTIGYW427 LCYINSTINP437 ACYALCNATF 447 KKTFKECALA497 KD
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Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .TPO or .TPO2 or .TPO3 or :3TPO;style chemicals stick;color identity;select .R:489 or .R:492; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
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| Ligand Name: LY2119620 | Ligand Info | |||||
| Structure Description | Muscarinic acetylcholine receptor 2-Go complex | PDB:6OIK | ||||
| Method | Electron microscopy | Resolution | 3.60 Å | Mutation | Yes | [3] |
| PDB Sequence |
TFEVVFIVLV
29 AGSLSLVTII39 GNILVMVSIK49 VNRHLQTVNN59 YFLFSLACAD69 LIIGVFSMNL 79 YTLYTVIGYW89 PLGPVVCDLW99 LALDYVVSNA109 SVMNLLIISF119 DRYFCVTKPL 129 TYPVKRTTKM139 AGMMIAAAWV149 LSFILWAPAI159 LFWQFIVGVR169 TVEDGECYIQ 179 FFSNAAVTFG189 TAIAAFYLPV199 IIMTVLYWHI209 SRASKSRIKP379 SREKKVTRTI 389 LAILLAFIIT399 WAPYNVMVLI409 NTFCAPCIPN419 TVWTIGYWLC429 YINSTINPAC 439 YALCNATFKK449 TFKHLLMC
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Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .2CU or .2CU2 or .2CU3 or :32CU;style chemicals stick;color identity;select .R:80 or .R:83 or .R:84 or .R:87 or .R:104 or .R:176 or .R:177 or .R:178 or .R:181 or .R:419 or .R:422 or .R:423 or .R:426; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| Ligand Name: Iperoxo | Ligand Info | |||||
| Structure Description | Structure of active human M2 muscarinic acetylcholine receptor bound to the agonist iperoxo | PDB:4MQS | ||||
| Method | X-ray diffraction | Resolution | 3.50 Å | Mutation | Yes | [4] |
| PDB Sequence |
KTFEVVFIVL
28 VAGSLSLVTI38 IGNILVMVSI48 KVNRHLQTVN58 NYFLFSLACA68 DLIIGVFSMN 78 LYTLYTVIGY88 WPLGPVVCDL98 WLALDYVVSN108 ASVMNLLIIS118 FDRYFCVTKP 128 LTYPVKRTTK138 MAGMMIAAAW148 VLSFILWAPA158 ILFWQFIVGV168 RTVEDGECYI 178 QFFSNAAVTF188 GTAIAAFYLP198 VIIMTVLYWH208 ISRASKSPPP379 SREKKVTRTI 389 LAILLAFIIT399 WAPYNVMVLI409 NTFCAPCIPN419 TVWTIGYWLC429 YINSTINPAC 439 YALCNATFKK449 TFKHLLM
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Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .IXO or .IXO2 or .IXO3 or :3IXO;style chemicals stick;color identity;select .A:103 or .A:104 or .A:107 or .A:108 or .A:111 or .A:155 or .A:194 or .A:195 or .A:400 or .A:403 or .A:404 or .A:426 or .A:429 or .A:430; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| Ligand Name: (3r)-1-Azabicyclo[2.2.2]oct-3-Yl Hydroxy(Diphenyl)acetate | Ligand Info | |||||
| Structure Description | Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist | PDB:3UON | ||||
| Method | X-ray diffraction | Resolution | 3.00 Å | Mutation | Yes | [1] |
| PDB Sequence |
TFEVVFIVLV
29 AGSLSLVTII39 GNILVMVSIK49 VNRHLQTVNN59 YFLFSLACAD69 LIIGVFSMNL 79 YTLYTVIGYW89 PLGPVVCDLW99 LALDYVVSNA109 SVMNLLIISF119 DRYFCVTKPL 129 TYPVKRTTKM139 AGMMIAAAWV149 LSFILWAPAI159 LFWQFIVGVR169 TVEDGECYIQ 179 FFSNAAVTFG189 TAIAAFYLPV199 IIMTVLYWHI209 SRASKSRINI1003 FEMLRIDEGL 1013 RLKIYKDTEG1023 YYTIGIGHLL1033 TKSPSLNAAK1043 SELDKAIGRN1053 TNGVITKDEA 1063 EKLFNQDVDA1073 AVRGILRNAK1083 LKPVYDSLDA1093 VRRAALINMV1103 FQMGETGVAG 1113 FTNSLRMLQQ1123 KRWDEAAVNL1133 AKSRWYNQTP1143 NRAKRVITTF1153 RTGTWDAYPP 378 PSREKKVTRT388 ILAILLAFII398 TWAPYNVMVL408 INTFCAPCIP418 NTVWTIGYWL 428 CYINSTINPA438 CYALCNATFK448 KTFKHLLM
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Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .QNB or .QNB2 or .QNB3 or :3QNB;style chemicals stick;color identity;select .A:103 or .A:104 or .A:107 or .A:108 or .A:111 or .A:155 or .A:181 or .A:187 or .A:190 or .A:191 or .A:194 or .A:195 or .A:400 or .A:403 or .A:404 or .A:407 or .A:426 or .A:429 or .A:430; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
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| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist. Nature. 2012 Jan 25;482(7386):547-51. | ||||
| REF 2 | Structure of the M2 muscarinic receptor-beta-arrestin complex in a lipid nanodisc. Nature. 2020 Mar;579(7798):297-302. | ||||
| REF 3 | Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes. Science. 2019 May 10;364(6440):552-557. | ||||
| REF 4 | Activation and allosteric modulation of a muscarinic acetylcholine receptor. Nature. 2013 Dec 5;504(7478):101-6. | ||||
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