Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T01385 | Target Info | |||
| Target Name | HUMAN valosin-containing protein p97 (VCP) | ||||
| Synonyms | Valosin-containing protein; Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit | ||||
| Gene Name | VCP | ||||
| Biochemical Class | AAA ATPase family | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | adenosine diphosphate | Ligand Info | |||
| Canonical SMILES | C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)O)O)O)N | ||||
| InChI | 1S/C10H15N5O10P2/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(24-10)1-23-27(21,22)25-26(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1 | ||||
| InChIKey | XTWYTFMLZFPYCI-KQYNXXCUSA-N | ||||
| PubChem Compound ID | 6022 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 5FTJ Cryo-EM structure of human p97 bound to UPCDC30245 inhibitor | ||||||
| Method | Electron microscopy | Resolution | 2.30 Å | Mutation | No | [1] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQ
|
|||||
|
|
ASP205
3.789
ILE206
4.113
GLY207
3.004
GLY208
4.526
PRO246
4.144
PRO247
4.176
GLY248
3.236
THR249
3.203
GLY250
3.158
LYS251
2.844
THR252
2.676
LEU253
3.431
ILE380
3.321
ILE383
3.996
HIS384
3.079
GLY408
3.178
ALA409
3.481
ALA412
4.204
ASP478
2.912
|
|||||
| PDB ID: 5FTK Cryo-EM structure of human p97 bound to ADP | ||||||
| Method | Electron microscopy | Resolution | 2.40 Å | Mutation | No | [1] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQ
|
|||||
|
|
ASP205
3.111
ILE206
3.664
GLY207
2.795
GLY208
3.496
CYS209
4.335
PRO246
4.653
PRO247
3.546
GLY248
2.621
THR249
3.235
GLY250
2.620
LYS251
2.581
THR252
2.911
LEU253
3.125
ILE380
3.682
ILE383
4.591
HIS384
2.912
GLY408
3.544
ALA409
3.335
ALA412
4.447
|
|||||
| PDB ID: 7LN0 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and Ub6 (Class 2) | ||||||
| Method | Electron microscopy | Resolution | 2.98 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
|
ASP205
3.232
ILE206
3.426
GLY207
2.327
GLY208
4.537
CYS209
4.833
PRO247
4.391
GLY248
3.053
THR249
2.847
GLY250
3.014
LYS251
3.177
THR252
2.337
LEU253
3.294
GLU305
3.857
ILE380
3.396
ILE383
4.674
HIS384
3.404
GLY408
3.663
ALA409
3.336
ASP478
4.055
|
|||||
| PDB ID: 7LN4 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and polyubiquitinated Ub-Eos (FOM, Class 3) | ||||||
| Method | Electron microscopy | Resolution | 3.00 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:254 or .A:304 or .A:305 or .A:348 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.492
ILE206
3.399
GLY207
3.325
PRO246
4.746
PRO247
3.574
GLY248
2.620
THR249
3.155
GLY250
2.485
LYS251
3.102
THR252
3.182
LEU253
3.541
ILE254
4.995
ASP304
4.542
GLU305
4.318
ASN348
4.608
ILE380
3.560
HIS384
2.564
GLY408
4.007
|
|||||
| PDB ID: 7LMZ Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and Ub6 (Class 1) | ||||||
| Method | Electron microscopy | Resolution | 3.06 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.350
ILE206
3.536
GLY207
2.312
GLY208
4.534
PRO246
4.972
PRO247
4.517
GLY248
3.068
THR249
2.876
GLY250
2.609
LYS251
3.146
THR252
3.070
LEU253
3.386
ASP304
4.391
GLU305
4.648
ILE380
3.531
ILE383
3.970
HIS384
3.287
GLY408
3.386
ALA409
3.589
ALA412
4.393
|
|||||
| PDB ID: 7LN5 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and polyubiquitinated Ub-Eos (CHAPSO, Class 1, Close State) | ||||||
| Method | Electron microscopy | Resolution | 3.09 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.886
ILE206
3.335
GLY207
2.907
PRO247
3.268
GLY248
3.097
THR249
3.441
GLY250
3.152
LYS251
2.677
THR252
3.391
LEU253
3.312
ASP304
4.687
GLU305
4.413
ILE380
3.388
ILE383
4.994
HIS384
3.091
GLY408
3.330
ALA409
3.732
ALA412
3.470
ASP478
3.614
|
|||||
| PDB ID: 5FTM Cryo-EM structure of human p97 bound to ATPgS (Conformation II) | ||||||
| Method | Electron microscopy | Resolution | 3.20 Å | Mutation | No | [1] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 5FTL Cryo-EM structure of human p97 bound to ATPgS (Conformation I) | ||||||
| Method | Electron microscopy | Resolution | 3.30 Å | Mutation | No | [1] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.133
ILE206
4.003
GLY207
3.237
CYS209
4.634
PRO246
4.615
PRO247
4.365
GLY248
3.308
THR249
3.413
GLY250
3.123
LYS251
2.941
THR252
2.792
LEU253
3.264
ILE380
4.083
ILE383
4.934
HIS384
3.164
GLY408
3.715
ALA409
3.505
ALA412
4.178
ASP478
3.015
|
|||||
| PDB ID: 7BPA Human AAA+ ATPase VCP mutant - T76A, AMP-PNP-bound form, Conformation I | ||||||
| Method | Electron microscopy | Resolution | 3.30 Å | Mutation | Yes | [3] |
| PDB Sequence |
KQKNRPNRLI
27 VDEAINEDNS37 VVSLSQPKMD47 ELQLFRGDTV57 LLKGKKRREA67 VCIVLSDDAC 77 SDEKIRMNRV87 VRNNLRVRLG97 DVISIQPCPD107 VKYGKRIHVL117 PIDDTVEGIT 127 GNLFEVYLKP137 YFLEAYRPIR147 KGDIFLVRGG157 MRAVEFKVVE167 TDPSPYCIVA 177 PDTVIHCEGE187 PIKREDEEES197 LNEVGYDDIG207 GCRKQLAQIK217 EMVELPLRHP 227 ALFKAIGVKP237 PRGILLYGPP247 GTGKTLIARA257 VANETGAFFF267 LINGPEIMSK 277 LAGESESNLR287 KAFEEAEKNA297 PAIIFIDELD307 AIAPKREKTH317 GEVERRIVSQ 327 LLTLMDGLKQ337 RAHVIVMAAT347 NRPNSIDPAL357 RRFGRFDREV367 DIGIPDATGR 377 LEILQIHTKN387 MKLADDVDLE397 QVANETHGHV407 GADLAALCSE417 AALQAIRKKM 427 DLIDLEDETI437 DAEVMNSLAV447 TMDDFRWALS457 QSNPSALRET467 VVEVPQVTWE 477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTMWFGESEA557 NVREIFDKAR567 QAAPCVLFFD 577 ELDSIAKARG587 GNIGDGGGAA597 DRVINQILTE607 MDGMSTKKNV617 FIIGATNRPD 627 IIDPAILRPG637 RLDQLIYIPL647 PDEKSRVAIL657 KANLRKSPVA667 KDVDLEFLAK 677 MTNGFSGADL687 TEICQRACKL697 AIRESIESEI707 RRERERQTEE724 DDPVPEIRRD 734 HFEEAMRFAR744 RSVSDNDIRK754 YEMFAQTLQQ764 SR
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:256 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7LN1 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and Ub6 (Class 3) | ||||||
| Method | Electron microscopy | Resolution | 3.40 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:348 or .A:380 or .A:384 or .A:407 or .A:408 or .A:409 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.548
ILE206
4.470
GLY207
3.288
PRO247
4.502
GLY248
2.833
THR249
3.256
GLY250
3.311
LYS251
3.315
THR252
3.007
LEU253
3.408
GLU305
3.350
ASN348
2.876
ILE380
3.078
HIS384
4.160
VAL407
4.861
GLY408
3.241
ALA409
3.071
ASP478
3.364
|
|||||
| PDB ID: 7LN3 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and polyubiquitinated Ub-Eos (FOM, Class 2) | ||||||
| Method | Electron microscopy | Resolution | 3.45 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:256 or .A:304 or .A:305 or .A:346 or .A:372 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
CYS209
4.948
PRO246
4.792
PRO247
3.910
GLY248
3.829
THR249
4.131
GLY250
3.134
LYS251
3.480
THR252
2.864
LEU253
3.723
ARG256
3.905
ASP304
2.964
GLU305
2.904
ALA346
4.711
PRO372
4.570
ILE380
3.252
HIS384
3.298
GLY408
2.929
ALA409
3.558
|
|||||
| PDB ID: 7LN6 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and polyubiquitinated Ub-Eos (CHAPSO, Class 2, Open State) | ||||||
| Method | Electron microscopy | Resolution | 3.58 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPP472 QVTWEDIGGL482 EDVKRELQEL 492 VQYPVEHPDK502 FLKFGMTPSK512 GVLFYGPPGC522 GKTLLAKAIA532 NECQANFISI 542 KGPELLTMWF552 GESEANVREI562 FDKARQAAPC572 VLFFDQLDSI582 AKARGGNIGD 592 GGGAADRVIN602 QILTEMDGMS612 TKKNVFIIGA622 TNRPDIIDPA632 ILRPGRLDQL 642 IYIPLPDEKS652 RVAILKANLR662 KSPVAKDVDL672 EFLAKMTNGF682 SGADLTEICQ 692 RACKLAIRES702 IESEIRRERE712 RQPVPEIRRD734 HFEEAMRFAR744 RSVSDNDIRK 754 YEMFAQTLQQ764 SRGFGSFRFP774 S
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:478 or .A:479 or .A:480 or .A:481 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:578 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.375
ILE206
4.359
GLY207
3.209
PRO246
4.724
PRO247
3.616
GLY248
2.571
THR249
2.962
GLY250
3.573
LYS251
3.038
THR252
2.521
LEU253
3.388
ASP304
4.116
GLU305
4.704
ASN348
3.638
ILE380
3.275
ILE383
4.827
HIS384
3.167
GLY408
3.583
ALA409
3.508
|
|||||
| PDB ID: 7BP9 Human AAA+ ATPase VCP mutant - T76E, ADP-bound form | ||||||
| Method | Electron microscopy | Resolution | 3.60 Å | Mutation | Yes | [3] |
| PDB Sequence |
KNRPNRLIVD
29 EAINEDNSVV39 SLSQPKMDEL49 QLFRGDTVLL59 KGKKRREAVC69 IVLSDDECSD 79 EKIRMNRVVR89 NNLRVRLGDV99 ISIQPCPDVK109 YGKRIHVLPI119 DDTVEGITGN 129 LFEVYLKPYF139 LEAYRPIRKG149 DIFLVRGGMR159 AVEFKVVETD169 PSPYCIVAPD 179 TVIHCEGEPI189 KREDEEESLN199 EVGYDDIGGC209 RKQLAQIKEM219 VELPLRHPAL 229 FKAIGVKPPR239 GILLYGPPGT249 GKTLIARAVA259 NETGAFFFLI269 NGPEIMSKLA 279 GESESNLRKA289 FEEAEKNAPA299 IIFIDELDAI309 APKREKTHGE319 VERRIVSQLL 329 TLMDGLKQRA339 HVIVMAATNR349 PNSIDPALRR359 FGRFDREVDI369 GIPDATGRLE 379 ILQIHTKNMK389 LADDVDLEQV399 ANETHGHVGA409 DLAALCSEAA419 LQAIRKKMDL 429 IDLEDETIDA439 EVMNSLAVTM449 DDFRWALSQS459 NPSALRETVV469 EVPQVTWEDI 479 GGLEDVKREL489 QELVQYPVEH499 PDKFLKFGMT509 PSKGVLFYGP519 PGCGKTLLAK 529 AIANECQANF539 ISIKGPELLT549 MWFGESEANV559 REIFDKARQA569 APCVLFFDEL 579 DSIAKARGGN589 IGDGGGAADR599 VINQILTEMD609 GMSTKKNVFI619 IGATNRPDII 629 DPAILRPGRL639 DQLIYIPLPD649 EKSRVAILKA659 NLRKSPVAKD669 VDLEFLAKMT 679 NGFSGADLTE689 ICQRACKLAI699 RESIESEIRR709 ERERQTEEDD726 PVPEIRRDHF 736 EEAMRFARRS746 VSDNDIRKYE756 MFAQTLQQSR766
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:256 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.361
ILE206
3.526
GLY207
3.493
GLY208
4.475
CYS209
4.403
PRO246
4.564
PRO247
4.167
GLY248
3.000
THR249
2.937
GLY250
3.240
LYS251
3.351
THR252
2.923
LEU253
2.995
ARG256
4.921
ASP304
4.877
ILE380
3.259
ILE383
4.612
HIS384
3.509
GLY408
3.475
ALA409
3.521
|
|||||
| PDB ID: 7MHS Structure of p97 (subunits A to E) with substrate engaged | ||||||
| Method | Electron microscopy | Resolution | 3.60 Å | Mutation | No | [4] |
| PDB Sequence |
EVGYDDIGGC
209 RKQLAQIKEM219 VELPLRHPAL229 FKAIGVKPPR239 GILLYGPPGT249 GKTLIARAVA 259 NETGAFFFLI269 NGPEIMSKLA279 GESESNLRKA289 FEEAEKNAPA299 IIFIDELDAI 309 APKREKTHGE319 VERRIVSQLL329 TLMDAHVIVM344 AATNRPNSID354 PALRRFGRFD 364 REVDIGIPDA374 TGRLEILQIH384 TKNMKLADDV394 DLEQVANETH404 GHVGADLAAL 414 CSEAALQAIR424 KKMDLIEVMN443 SLAVTMDDFR453 WALSQSNPSA463 LRETVVEVPQ 473 VTWEDIGGLE483 DVKRELQELV493 QYPVEHPDKF503 LKFGMTPSKG513 VLFYGPPGCG 523 KTLLAKAIAN533 ECQANFISIK543 GPELLTMWFG553 ESEANVREIF563 DKARQAAPCV 573 LFFDELDSIA583 KARGGGGGAA597 DRVINQILTE607 MDGMSTKKNV617 FIIGATNRPD 627 IIDPAILRPG637 RLDQLIYIPL647 PDEKSRVAIL657 KANLRKSPVA667 KDVDLEFLAK 677 MTNGFSGADL687 TEICQRACKL697 AIRESIESEI707 RRERERQDPV728 PEIRRDHFEE 738 AMRFARRSVS748 DNDIRKYEMF758 AQTL
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:256 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
4.033
ILE206
3.961
GLY207
3.259
PRO246
4.763
PRO247
4.208
GLY248
3.166
THR249
2.975
GLY250
3.139
LYS251
3.380
THR252
2.220
LEU253
2.665
ARG256
3.495
ILE380
3.846
HIS384
3.372
GLY408
4.502
ALA409
3.218
ALA412
4.971
|
|||||
| PDB ID: 7LN2 Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and polyubiquitinated Ub-Eos (FOM, Class 1) | ||||||
| Method | Electron microscopy | Resolution | 3.63 Å | Mutation | Yes | [2] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKE 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNPV471 PQVTWEDIGG481 LEDVKRELQE 491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG521 CGKTLLAKAI531 ANECQANFIS 541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP571 CVLFFDQLDS581 IAKARGGNIG 591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG621 ATNRPDIIDP631 AILRPGRLDQ 641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD671 LEFLAKMTNG681 FSGADLTEIC 691 QRACKLAIRE701 SIESEIRRER711 ERQPVPEIRR733 DHFEEAMRFA743 RRSVSDNDIR 753 KYEMFAQTLQ763 QSRGFGSFRF773 PS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:256 or .A:305 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:529 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
GLY248
4.006
THR249
4.010
GLY250
3.147
LYS251
4.841
THR252
3.108
LEU253
3.451
ARG256
3.896
GLU305
4.777
ILE380
3.455
HIS384
3.807
GLY408
3.533
ALA409
3.571
ALA412
4.021
ASP478
3.217
ILE479
3.998
GLY480
2.901
|
|||||
| PDB ID: 7BP8 Human AAA+ ATPase VCP mutant - T76A, ADP-bound form | ||||||
| Method | Electron microscopy | Resolution | 3.90 Å | Mutation | Yes | [3] |
| PDB Sequence |
KNRPNRLIVD
29 EAINEDNSVV39 SLSQPKMDEL49 QLFRGDTVLL59 KGKKRREAVC69 IVLSDDACSD 79 EKIRMNRVVR89 NNLRVRLGDV99 ISIQPCPDVK109 YGKRIHVLPI119 DDTVEGITGN 129 LFEVYLKPYF139 LEAYRPIRKG149 DIFLVRGGMR159 AVEFKVVETD169 PSPYCIVAPD 179 TVIHCEGEPI189 KREDEEESLN199 EVGYDDIGGC209 RKQLAQIKEM219 VELPLRHPAL 229 FKAIGVKPPR239 GILLYGPPGT249 GKTLIARAVA259 NETGAFFFLI269 NGPEIMSKLA 279 GESESNLRKA289 FEEAEKNAPA299 IIFIDELDAI309 APKREKTHGE319 VERRIVSQLL 329 TLMDGLKQRA339 HVIVMAATNR349 PNSIDPALRR359 FGRFDREVDI369 GIPDATGRLE 379 ILQIHTKNMK389 LADDVDLEQV399 ANETHGHVGA409 DLAALCSEAA419 LQAIRKKMDL 429 IDLEDETIDA439 EVMNSLAVTM449 DDFRWALSQS459 NPSALRETVV469 EVPQVTWEDI 479 GGLEDVKREL489 QELVQYPVEH499 PDKFLKFGMT509 PSKGVLFYGP519 PGCGKTLLAK 529 AIANECQANF539 ISIKGPELLT549 MWFGESEANV559 REIFDKARQA569 APCVLFFDEL 579 DSIAKARGGN589 IGDGGGAADR599 VINQILTEMD609 GMSTKKNVFI619 IGATNRPDII 629 DPAILRPGRL639 DQLIYIPLPD649 EKSRVAILKA659 NLRKSPVAKD669 VDLEFLAKMT 679 NGFSGADLTE689 ICQRACKLAI699 RESIESEIRR709 ERERQTEEDD726 PVPEIRRDHF 736 EEAMRFARRS746 VSDNDIRKYE756 MFAQTLQQSR766
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:256 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:624 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.508
ILE206
3.430
GLY207
2.482
GLY208
4.703
CYS209
4.799
GLY248
4.105
THR249
3.273
GLY250
3.291
LYS251
3.797
THR252
2.781
LEU253
3.760
ARG256
3.309
ILE380
3.970
ILE383
4.393
HIS384
3.679
GLY408
3.519
ALA409
3.574
ALA412
4.610
|
|||||
| PDB ID: 7MDO Structure of human p97 ATPase L464P mutant | ||||||
| Method | Electron microscopy | Resolution | 4.12 Å | Mutation | Yes | [5] |
| PDB Sequence |
EESLNEVGYD
204 DIGGCRKQLA214 QIKEMVELPL224 RHPALFKAIG234 VKPPRGILLY244 GPPGTGKTLI 254 ARAVANETGA264 FFFLINGPEI274 MSKLAGESES284 NLRKAFEEAE294 KNAPAIIFID 304 ELDAIAPKRE314 KTHGEVERRI324 VSQLLTLMDG334 LKQRAHVIVM344 AATNRPNSID 354 PALRRFGRFD364 REVDIGIPDA374 TGRLEILQIH384 TKNMKLADDV394 DLEQVANETH 404 GHVGADLAAL414 CSEAALQAIR424 KKMDLIDLED434 ETIDAEVMNS444 LAVTMDDFRW 454 ALSQSNPSAP464 RETVVEVPQV474 TWEDIGGLED484 VKRELQELVQ494 YPVEHPDKFL 504 KFGMTPSKGV514 LFYGPPGCGK524 TLLAKAIANE534 CQANFISIKG544 PELLTMWFGE 554 SEANVREIFD564 KARQAAPCVL574 FFDELDSIDR599 VINQILTEMD609 GMSTKKNVFI 619 IGATNRPDII629 DPAILRPGRL639 DQLIYIPLPD649 EKSRVAILKA659 NLRKSPVAKD 669 VDLEFLAKMT679 NGFSGADLTE689 ICQRACKLAI699 RESIESEIIR732 RDHFEEAMRF 742 ARRSVSDNDI752 RKYEMFAQTL762 Q
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7BPB Human AAA+ ATPase VCP mutant - T76E, AMP-PNP bound form, Conformation I | ||||||
| Method | Electron microscopy | Resolution | 4.30 Å | Mutation | Yes | [3] |
| PDB Sequence |
KNRPNRLIVD
29 EAINEDNSVV39 SLSQPKMDEL49 QLFRGDTVLL59 KGKKRREAVC69 IVLSDDECSD 79 EKIRMNRVVR89 NNLRVRLGDV99 ISIQPCPDVK109 YGKRIHVLPI119 DDTVEGITGN 129 LFEVYLKPYF139 LEAYRPIRKG149 DIFLVRGGMR159 AVEFKVVETD169 PSPYCIVAPD 179 TVIHCEGEPI189 KREDEEESLN199 EVGYDDIGGC209 RKQLAQIKEM219 VELPLRHPAL 229 FKAIGVKPPR239 GILLYGPPGT249 GKTLIARAVA259 NETGAFFFLI269 NGPEIMSKLA 279 GESESNLRKA289 FEEAEKNAPA299 IIFIDELDAI309 APKREKTHGE319 VERRIVSQLL 329 TLMDGLKQRA339 HVIVMAATNR349 PNSIDPALRR359 FGRFDREVDI369 GIPDATGRLE 379 ILQIHTKNMK389 LADDVDLEQV399 ANETHGHVGA409 DLAALCSEAA419 LQAIRKKMDL 429 IDLEDETIDA439 EVMNSLAVTM449 DDFRWALSQS459 NPSALRETVV469 EVPQVTWEDI 479 GGLEDVKREL489 QELVQYPVEH499 PDKFLKFGMT509 PSKGVLFYGP519 PGCGKTLLAK 529 AIANECQANF539 ISIKGPELLT549 MWFGESEANV559 REIFDKARQA569 APCVLFFDEL 579 DSIAKARGGN589 IGDGGGAADR599 VINQILTEMD609 GMSTKKNVFI619 IGATNRPDII 629 DPAILRPGRL639 DQLIYIPLPD649 EKSRVAILKA659 NLRKSPVAKD669 VDLEFLAKMT 679 NGFSGADLTE689 ICQRACKLAI699 RESIESEIRR709 ERERQTEEDD726 PVPEIRRDHF 736 EEAMRFARRS746 VSDNDIRKYE756 MFAQTLQQSR766
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7R7T p47-bound p97-R155H mutant with ADP | ||||||
| Method | Electron microscopy | Resolution | 4.50 Å | Mutation | Yes | [6] |
| PDB Sequence |
NRLIVDEAIN
33 EDNSVVSLSQ43 PKMDELQLFR53 GDTVLLKGKK63 RREAVCIVLS73 DDTCSDEKIR 83 MNRVVRNNLR93 VRLGDVISIQ103 PCPDVKYGKR113 IHVLPIDDTV123 EGITGNLFEV 133 YLKPYFLEAY143 RPIRKGDIFL153 VHGGMRAVEF163 KVVETDPSPY173 CIVAPDTVIH 183 CEGEPIKRED193 EEESLNEVGY203 DDIGGCRKQL213 AQIKEMVELP223 LRHPALFKAI 233 GVKPPRGILL243 YGPPGTGKTL253 IARAVANETG263 AFFFLINGPE273 IMSKLAGESE 283 SNLRKAFEEA293 EKNAPAIIFI303 DELDAIAPKR313 EKTHGEVERR323 IVSQLLTLMD 333 GLKQRAHVIV343 MAATNRPNSI353 DPALRRFGRF363 DREVDIGIPD373 ATGRLEILQI 383 HTKNMKLADD393 VDLEQVANET403 HGHVGADLAA413 LCSEAALQAI423 RKKMDLIDLD 438 AEVMNSLAVT448 MDDFRWALSQ458 SNPSALRETV468 VEVPQVTWED478 IGGLEDVKRE 488 LQELVQYPVE498 HPDKFLKFGM508 TPSKGVLFYG518 PPGCGKTLLA528 KAIANECQAN 538 FISIKGPELL548 TMWFGESEAN558 VREIFDKARQ568 AAPCVLFFDE578 LDSIAKARGG 588 NGAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRRERER713 DPVPEIRRDH735 FEEAMRFARR745 SVSDNDIRKY 755 EMFAQTLQQS765 RGFGSFRFP
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:254 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:522 or .A:523 or .A:526 or .A:656 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
4.178
ILE206
3.666
GLY207
2.770
CYS209
3.741
THR249
3.071
GLY250
3.255
LYS251
3.097
THR252
2.708
LEU253
2.535
ILE254
3.159
ILE380
3.587
HIS384
3.135
|
|||||
| PDB ID: 7MDM Structure of human p97 ATPase L464P mutant | ||||||
| Method | Electron microscopy | Resolution | 4.86 Å | Mutation | Yes | [5] |
| PDB Sequence |
RLIVDEAINE
34 DNSVVSLSQP44 KMDELQLFRG54 DTVLLKGKKR64 REAVCIVLSD74 DTCSDEKIRM 84 NRVVRNNLRV94 RLGDVISIQP104 CPDVKYGKRI114 HVLPIDDTVE124 GITGNLFEVY 134 LKPYFLEAYR144 PIRKGDIFLV154 RGGMRAVEFK164 VVETDPSPYC174 IVAPDTVIHC 184 EGEPIKREDE194 EESLNEVGYD204 DIGGCRKQLA214 QIKEMVELPL224 RHPALFKAIG 234 VKPPRGILLY244 GPPGTGKTLI254 ARAVANETGA264 FFFLINGPEI274 MSKLAGESES 284 NLRKAFEEAE294 KNAPAIIFID304 ELDAIAPKRE314 KTHGEVERRI324 VSQLLTLMDG 334 LKQRAHVIVM344 AATNRPNSID354 PALRRFGRFD364 REVDIGIPDA374 TGRLEILQIH 384 TKNMKLADDV394 DLEQVANETH404 GHVGADLAAL414 CSEAALQAIR424 KKMDLIDLED 434 ETIDAEVMNS444 LAVTMDDFRW454 ALSQSNPSAP464 RETVVEVPQV474 TWEDIGGLED 484 VKRELQELVQ494 YPVEHPDKFL504 KFGMTPSKGV514 LFYGPPGCGK524 TLLAKAIANE 534 CQANFISIKG544 PELLTMWFGE554 SEANVREIFD564 KARQAAPCVL574 FFDELDSIDR 599 VINQILTEMD609 GMSTKKNVFI619 IGATNRPDII629 DPAILRPGRL639 DQLIYIPLPD 649 EKSRVAILKA659 NLRKSPVAKD669 VDLEFLAKMT679 NGFSGADLTE689 ICQRACKLAI 699 RESIESEIIR732 RDHFEEAMRF742 ARRSVSDNDI752 RKYEMFAQTL762 Q |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .B:205 or .B:206 or .B:207 or .B:208 or .B:248 or .B:249 or .B:250 or .B:251 or .B:252 or .B:253 or .B:380 or .B:407 or .B:408 or .B:409; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 5IFW Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death | ||||||
| Method | X-ray diffraction | Resolution | 3.40 Å | Mutation | No | [7] |
| PDB Sequence |
STAILKQKNR
22 PNRLIVDEAI32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL 72 SDDTCSDEKI82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT 122 VEGITGNLFE132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP 172 YCIVAPDTVI182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL 222 PLRHPALFKA232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP 272 EIMSKLAGES282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPG318 EVERRIVSQL 328 LTLMDGLKQR338 AHVIVMAATN348 RPNSIDPALR358 RFGRFDREVD368 IGIPDATGRL 378 EILQIHTKNM388 KLADDVDLEQ398 VANETHGHVG408 ADLAALCSEA418 ALQAIRKKDE 435 TIDAEVMNSL445 AVTMDDFRWA455 LSQSNPSALR465 ETVVEVPQVT475 WEDIGGLEDV 485 KRELQELVQK512 GVLFYGPPGC522 GKTLLAKAIA532 NECQANFISI542 KGPELLTMWF 552 GESEANVREI562 FDKARQAAPC572 VLFFDELDSI582 AKDRVINQIL605 TEMDGMSNVF 618 IIGATNRPDI628 IDPAILRPGR638 LDQLIYIPLP648 DEKSRVAILK658 ANLRKSPVAK 668 DVDLEFLAKM678 TNGFSGADLT688 EICQRACKLA698 IRESIESEIR708 RERERQTNPS 718 AMEVEEDDPV728 PEIRRDHFEE738 AMRFARRSVS748 DNDIRKYEMF758 AQ |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .B:205 or .B:206 or .B:207 or .B:208 or .B:209 or .B:246 or .B:247 or .B:248 or .B:249 or .B:250 or .B:251 or .B:252 or .B:253 or .B:254 or .B:380 or .B:383 or .B:384 or .B:408 or .B:409 or .B:412 or .B:478 or .B:479 or .B:480 or .B:482 or .B:519 or .B:520 or .B:521 or .B:522 or .B:523 or .B:524 or .B:525 or .B:526 or .B:577 or .B:656 or .B:684 or .B:685 or .B:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.483
ILE206
3.839
GLY207
2.236
GLY208
4.444
CYS209
4.579
PRO246
4.792
PRO247
3.480
GLY248
2.470
THR249
2.639
GLY250
2.349
LYS251
3.106
THR252
2.808
LEU253
2.694
ILE254
4.947
ILE380
3.804
ILE383
4.395
HIS384
3.147
GLY408
3.346
ALA409
3.104
|
|||||
| PDB ID: 7VCU Human p97 double hexamer conformer I with D1-ATPgammaS and D2-ADP bound | ||||||
| Method | Electron microscopy | Resolution | 3.15 Å | Mutation | No | [8] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIRRE710 RERQTNPSAM720 EVEEDDPVPE 730 IRRDHFEEAM740 RFARRSVSDN750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPSGN |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .K:478 or .K:479 or .K:480 or .K:482 or .K:519 or .K:520 or .K:521 or .K:522 or .K:523 or .K:524 or .K:525 or .K:526 or .K:577 or .K:624 or .K:656 or .K:660 or .K:684 or .K:685 or .K:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7VCT Human p97 single hexamer conformer III with D1-ATPgammaS and D2-ADP bound | ||||||
| Method | Electron microscopy | Resolution | 3.21 Å | Mutation | No | [8] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIRRE710 RERQTNPSAM720 EVEEDDPVPE 730 IRRDHFEEAM740 RFARRSVSDN750 DIRKYEMFAQ760 TLQQSRGFGS770 F |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:648 or .A:652 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7RLI Cryo-EM structure of human p97 bound to CB-5083 and ADP. | ||||||
| Method | Electron microscopy | Resolution | 3.10 Å | Mutation | No | [9] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7RL9 Cryo-EM structure of human p97-R191Q mutant bound to ADP. | ||||||
| Method | Electron microscopy | Resolution | 3.30 Å | Mutation | Yes | [9] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKQ191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIVPEI731 RRDHFEEAMR 741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGFGSF771 RFPS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:624 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.703
ILE206
3.968
GLY207
3.074
CYS209
4.287
PRO246
4.812
PRO247
3.963
GLY248
3.102
THR249
2.476
GLY250
2.902
LYS251
2.112
THR252
2.948
LEU253
3.504
ASP304
4.813
ILE380
4.202
ILE383
4.006
HIS384
2.749
GLY408
3.202
ALA409
3.500
ALA412
4.627
|
|||||
| PDB ID: 7RLB Cryo-EM structure of human p97-A232E mutant bound to ADP | ||||||
| Method | Electron microscopy | Resolution | 3.30 Å | Mutation | Yes | [9] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KEIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.405
ILE206
4.053
GLY207
3.365
CYS209
4.995
PRO246
4.637
PRO247
4.064
GLY248
2.808
THR249
2.689
GLY250
2.332
LYS251
3.004
THR252
2.690
LEU253
3.548
ASP304
4.597
ILE380
3.743
HIS384
2.915
GLY408
3.645
ALA409
3.608
ALA412
4.381
|
|||||
| PDB ID: 7RLD Cryo-EM structure of human p97-E470D mutant bound to ADP. | ||||||
| Method | Electron microscopy | Resolution | 3.40 Å | Mutation | Yes | [9] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVD470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.354
ILE206
4.717
GLY207
4.358
PRO247
3.634
GLY248
2.643
THR249
2.922
GLY250
3.014
LYS251
2.408
THR252
3.089
LEU253
3.559
ASP304
4.489
ILE380
3.318
ILE383
4.734
HIS384
2.878
GLY408
3.977
ALA409
3.936
ALA412
4.700
|
|||||
| PDB ID: 7RLG Cryo-EM structure of human p97-D592N mutant bound to ADP. | ||||||
| Method | Electron microscopy | Resolution | 3.70 Å | Mutation | Yes | [9] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GNGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.230
ILE206
3.888
GLY207
2.647
GLY208
4.900
CYS209
4.394
PRO247
3.919
GLY248
2.789
THR249
2.889
GLY250
3.123
LYS251
2.394
THR252
2.433
LEU253
3.377
ASP304
4.291
ILE380
3.957
ILE383
4.603
HIS384
3.272
GLY408
3.433
ALA409
3.546
|
|||||
| PDB ID: 7RL6 Cryo-EM structure of human p97-R155H mutant bound to ADP. | ||||||
| Method | Electron microscopy | Resolution | 3.70 Å | Mutation | Yes | [9] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVHGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.611
ILE206
3.891
GLY207
2.947
PRO246
4.585
PRO247
3.535
GLY248
2.065
THR249
2.724
GLY250
2.532
LYS251
2.868
THR252
3.128
LEU253
3.937
ASP304
4.476
ILE380
2.792
ILE383
4.464
HIS384
3.299
GLY408
3.545
ALA409
3.447
ALA412
4.404
ASP478
2.629
|
|||||
| PDB ID: 4KOD Structure of p97 N-D1 R155H mutant in complex with ADP | ||||||
| Method | X-ray diffraction | Resolution | 2.96 Å | Mutation | Yes | [10] |
| PDB Sequence |
RPNRLIVDEA
31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV71 LSDDTCSDEK 81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD121 TVEGITGNLF 131 EVYLKPYFLE141 AYRPIRKGDI151 FLVHGGMRAV161 EFKVVETDPS171 PYCIVAPDTV 181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE221 LPLRHPALFK 231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING271 PEIMSKLAGE 281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE321 RRIVSQLLTL 331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI371 PDATGRLEIL 381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ421 AIRKKMDLID 431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 5DYI Structure of p97 N-D1 wild-type in complex with ADP | ||||||
| Method | X-ray diffraction | Resolution | 3.71 Å | Mutation | No | [11] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:346 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
2.958
ILE206
3.679
GLY207
2.971
CYS209
4.905
PRO246
4.403
PRO247
3.894
GLY248
2.910
THR249
3.219
GLY250
3.071
LYS251
2.891
|
|||||
| PDB ID: 5IFS Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death | ||||||
| Method | X-ray diffraction | Resolution | 2.46 Å | Mutation | No | [7] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMEDE 435 TIDAEVMNSL445 AVTMDDFRWA455 LSQSNPQVTW476 EDIG
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .B:205 or .B:206 or .B:207 or .B:209 or .B:246 or .B:247 or .B:248 or .B:249 or .B:250 or .B:251 or .B:252 or .B:253 or .B:380 or .B:383 or .B:384 or .B:408 or .B:409 or .B:412 or .B:480; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6HD0 Common mode of remodeling AAA ATPases p97/CDC48 by their disassembly cofactors ASPL/PUX1 | ||||||
| Method | X-ray diffraction | Resolution | 3.73 Å | Mutation | No | [12] |
| PDB Sequence |
PNRLIVDEAI
32 NEDNSVVSLS42 QPKMDELQLF52 RGDTVLLKGK62 KRREAVCIVL72 SDDTCSDEKI 82 RMNRVVRNNL92 RVRLGDVISI102 QPCPDVKYGK112 RIHVLPIDDT122 VEGITGNLFE 132 VYLKPYFLEA142 YRPIRKGDIF152 LVRGGMRAVE162 FKVVETDPSP172 YCIVAPDTVI 182 HCEGEPIKRE192 DEEESLNEVG202 YDDIGGCRKQ212 LAQIKEMVEL222 PLRHPALFKA 232 IGVKPPRGIL242 LYGPPGTGKT252 LIARAVANET262 GAFFFLINGP272 EIMSKLAGES 282 ESNLRKAFEE292 AEKNAPAIIF302 IDELDAIAPK312 REKTHGEVER322 RIVSQLLTLM 332 DGLKQRAHVI342 VMAATNRPNS352 IDPALRRFGR362 FDREVDIGIP372 DATGRLEILQ 382 IHTKNMKLAD392 DVDLEQVANE402 THGHVGADLA412 ALCSEAALQA422 IRKKMDLIDL 432 EDETIDAEVM442 NSLAVTMDDF452 RWALSQSNP
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .B:205 or .B:206 or .B:207 or .B:208 or .B:247 or .B:248 or .B:249 or .B:250 or .B:251 or .B:252 or .B:253 or .B:304 or .B:380 or .B:384 or .B:408 or .B:409 or .B:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 5DYG Structure of p97 N-D1 L198W mutant in complex with ADP | ||||||
| Method | X-ray diffraction | Resolution | 2.20 Å | Mutation | Yes | [11] |
| PDB Sequence |
AILKQKNRPN
24 RLIVDEAINE34 DNSVVSLSQP44 KMDELQLFRG54 DTVLLKGKKR64 REAVCIVLSD 74 DTCSDEKIRM84 NRVVRNNLRV94 RLGDVISIQP104 CPDVKYGKRI114 HVLPIDDTVE 124 GITGNLFEVY134 LKPYFLEAYR144 PIRKGDIFLV154 RGGMRAVEFK164 VVETDPSPYC 174 IVAPDTVIHC184 EGEPIKREDE194 EESWNEVGYD204 DIGGCRKQLA214 QIKEMVELPL 224 RHPALFKAIG234 VKPPRGILLY244 GPPGTGKTLI254 ARAVANETGA264 FFFLINGPEI 274 MSKLAGESES284 NLRKAFEEAE294 KNAPAIIFID304 ELDAIAPKRE314 KTHGEVERRI 324 VSQLLTLMDG334 LKQRAHVIVM344 AATNRPNSID354 PALRRFGRFD364 REVDIGIPDA 374 TGRLEILQIH384 TKNMKLADDV394 DLEQVANETH404 GHVGADLAAL414 CSEAALQAIR 424 KKMDLIDLED434 ETIDAEVMNS444 LAVTMDDFRW454 ALSQSN
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6G2Z Crystal structure of the p97 D2 domain in a helical split-washer conformation | ||||||
| Method | X-ray diffraction | Resolution | 1.92 Å | Mutation | No | [13] |
| PDB Sequence |
VVEVPQVTWE
477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTSEANVREI562 FDKARQAAPC572 VLFFDELDSI 582 AKAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRREREP727 VPEIRRDHFE737 EAMRFARRSV747 SDNDIRKYEM 757 FAQTLQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6G2X Crystal structure of the p97 D2 domain in a helical split-washer conformation | ||||||
| Method | X-ray diffraction | Resolution | 2.08 Å | Mutation | No | [13] |
| PDB Sequence |
VVEVPQVTWE
477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTSEANVREI562 FDKARQAAPC572 VLFFDELDSI 582 AKAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRREREP727 VPEIRRDHFE737 EAMRFARRSV747 SDNDIRKYEM 757 FAQTLQQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6G30 Crystal structure of the p97 D2 domain in a helical split-washer conformation | ||||||
| Method | X-ray diffraction | Resolution | 2.42 Å | Mutation | No | [13] |
| PDB Sequence |
VVEVPQVTWE
477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTSEANVREI562 FDKARQAAPC572 VLFFDELDSI 582 AKAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRREREP727 VPEIRRDHFE737 EAMRFARRSV747 SDNDIRKYEM 757 FAQTLQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6G2W Crystal structure of the p97 D2 domain in a helical split-washer conformation | ||||||
| Method | X-ray diffraction | Resolution | 2.68 Å | Mutation | No | [13] |
| PDB Sequence |
VVEVPQVTWE
477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTSEANVREI562 FDKARQAAPC572 VLFFDELDSI 582 AKAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRREREP727 VPEIRRDHFE737 EAMRFARRSV747 SDNDIRKYEM 757 FAQTLQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:481 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6G2Y Crystal structure of the p97 D2 domain in a helical split-washer conformation | ||||||
| Method | X-ray diffraction | Resolution | 2.15 Å | Mutation | No | [13] |
| PDB Sequence |
VVEVPQVTWE
477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTSEANVREI562 FDKARQAAPC572 VLFFDELDSI 582 AKAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRREREP727 VPEIRRDHFE737 EAMRFARRSV747 SDNDIRKYEM 757 FAQTLQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 6G2V Crystal structure of the p97 D2 domain in a helical split-washer conformation | ||||||
| Method | X-ray diffraction | Resolution | 1.90 Å | Mutation | No | [13] |
| PDB Sequence |
VVEVPQVTWE
477 DIGGLEDVKR487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL 527 AKAIANECQA537 NFISIKGPEL547 LTSEANVREI562 FDKARQAAPC572 VLFFDELDSI 582 AKAADRVINQ603 ILTEMDGMST613 KKNVFIIGAT623 NRPDIIDPAI633 LRPGRLDQLI 643 YIPLPDEKSR653 VAILKANLRK663 SPVAKDVDLE673 FLAKMTNGFS683 GADLTEICQR 693 ACKLAIRESI703 ESEIRREREV728 PEIRRDHFEE738 AMRFARRSVS748 DNDIRKYEMF 758 AQTLQQ
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:624 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | 2.3 ? resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. Science. 2016 Feb 19;351(6275):871-5. | ||||
| REF 2 | Mechanistic insight into substrate processing and allosteric inhibition of human p97. Nat Struct Mol Biol. 2021 Jul;28(7):614-625. | ||||
| REF 3 | The phosphorylation and dephosphorylation switch of VCP/p97 regulates the architecture of centrosome and spindle. Cell Death Differ. 2022 Oct;29(10):2070-2088. | ||||
| REF 4 | Active conformation of the p97-p47 unfoldase complex. Nat Commun. 2022 May 12;13(1):2640. | ||||
| REF 5 | Conserved L464 in p97 D1-D2 linker is critical for p97 cofactor regulated ATPase activity. Biochem J. 2021 Sep 17;478(17):3185-3204. | ||||
| REF 6 | Structural and Functional Analysis of Disease-Linked p97 ATPase Mutant Complexes. Int J Mol Sci. 2021 Jul 28;22(15):8079. | ||||
| REF 7 | Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers. Nat Commun. 2016 Oct 20;7:13047. | ||||
| REF 8 | Cryo-EM structures of human p97 double hexamer capture potentiated ATPase-competent state. Cell Discov. 2022 Feb 22;8(1):19. | ||||
| REF 9 | AAA+ ATPase p97/VCP mutants and inhibitor binding disrupt inter-domain coupling and subsequent allosteric activation. J Biol Chem. 2021 Oct;297(4):101187. | ||||
| REF 10 | Altered intersubunit communication is the molecular basis for functional defects of pathogenic p97 mutants. J Biol Chem. 2013 Dec 20;288(51):36624-35. | ||||
| REF 11 | Role of the D1-D2 Linker of Human VCP/p97 in the Asymmetry and ATPase Activity of the D1-domain. Sci Rep. 2016 Jan 28;6:20037. | ||||
| REF 12 | Common Mode of Remodeling AAA ATPases p97/CDC48 by Their Disassembling Cofactors ASPL/PUX1. Structure. 2019 Dec 3;27(12):1830-1841.e3. | ||||
| REF 13 | Crystal structure of the catalytic D2 domain of the AAA+ ATPase p97 reveals a putative helical split-washer-type mechanism for substrate unfolding. FEBS Lett. 2020 Mar;594(5):933-943. | ||||
If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.