Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T12646 | Target Info | |||
| Target Name | Valosin-containing protein p97 (VCP) | ||||
| Synonyms | Valosin-containing protein; Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit | ||||
| Target Type | Clinical trial Target | ||||
| Gene Name | VCP | ||||
| Biochemical Class | Acid anhydride hydrolase | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | Phosphothiophosphoric acid-adenylate ester | Ligand Info | |||
| Canonical SMILES | C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=S)(O)O)O)O)N | ||||
| InChI | 1S/C10H16N5O12P3S/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(25-10)1-24-28(18,19)26-29(20,21)27-30(22,23)31/h2-4,6-7,10,16-17H,1H2,(H,18,19)(H,20,21)(H2,11,12,13)(H2,22,23,31)/t4-,6-,7-,10-/m1/s1 | ||||
| InChIKey | NLTUCYMLOPLUHL-KQYNXXCUSA-N | ||||
| PubChem Compound ID | 440317 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 7JY5 Structure of human p97 in complex with ATPgammaS and Npl4/Ufd1 (masked around p97) | ||||||
| Method | Electron microscopy | Resolution | 2.89 Å | Mutation | No | [1] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMA557 NVREIFDKAR567 QAAPCVLFFD 577 ELDSIAKARG595 AADRVINQIL605 TEMDGMSTKK615 NVFIIGATNR625 PDIIDPAILR 635 PGRLDQLIYI645 PLPDEKSRVA655 ILKANLRKSP665 VAKDVDLEFL675 AKMTNGFSGA 685 DLTEICQRAC695 KLAIRESIES705 EIRRERERQP727 VPEIRRDHFE737 EAMRFARRSV 747 SDNDIRKYEM757 FAQTLQQSRG767 F
|
|||||
|
|
ASP205
3.234
ILE206
3.602
GLY207
2.894
CYS209
4.009
PRO246
4.549
PRO247
3.747
GLY248
2.714
THR249
3.364
GLY250
3.195
LYS251
2.789
THR252
2.614
LEU253
3.096
GLU305
4.265
ASN348
2.994
ILE380
3.798
ILE383
4.401
HIS384
3.060
GLY408
3.478
ALA409
3.495
ALA412
4.295
|
|||||
| PDB ID: 7RLH Cryo-EM structure of human p97-D592N mutant bound to ATPgS. | ||||||
| Method | Electron microscopy | Resolution | 3.00 Å | Mutation | Yes | [2] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GNGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGF
|
|||||
|
|
ASP205
3.259
ILE206
3.682
GLY207
2.892
CYS209
4.620
PRO246
4.782
PRO247
3.670
GLY248
2.727
THR249
3.326
GLY250
2.681
LYS251
2.823
THR252
2.783
LEU253
3.141
ASP304
4.536
ASN348
3.144
ILE380
3.745
ILE383
4.710
HIS384
3.203
GLY408
3.387
ALA409
3.262
ALA412
4.288
|
|||||
| PDB ID: 7RLF Cryo-EM structure of human p97-E470D mutant bound to ATPgS. | ||||||
| Method | Electron microscopy | Resolution | 3.10 Å | Mutation | Yes | [2] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVD470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGF
|
|||||
|
|
ASP205
3.268
ILE206
3.749
GLY207
3.009
CYS209
4.711
PRO246
4.702
PRO247
3.725
GLY248
2.914
THR249
3.419
GLY250
2.772
LYS251
2.597
THR252
2.679
LEU253
3.367
ASP304
4.357
ASN348
2.716
ILE380
3.770
ILE383
4.599
HIS384
3.157
GLY408
3.446
ALA409
3.442
ALA412
4.304
|
|||||
| PDB ID: 5FTM Cryo-EM structure of human p97 bound to ATPgS (Conformation II) | ||||||
| Method | Electron microscopy | Resolution | 3.20 Å | Mutation | No | [3] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:624 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 5FTN Cryo-EM structure of human p97 bound to ATPgS (Conformation III) | ||||||
| Method | Electron microscopy | Resolution | 3.30 Å | Mutation | No | [3] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIVPEI731 RRDHFEEAMR 741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:624 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.230
ILE206
3.363
GLY207
2.773
GLY208
4.938
PRO246
4.486
PRO247
3.514
GLY248
2.537
THR249
2.804
GLY250
2.413
LYS251
2.963
THR252
2.950
LEU253
3.533
ASN348
3.072
ILE380
3.783
ILE383
4.805
HIS384
3.671
GLY408
3.544
ALA409
3.445
ALA412
4.201
|
|||||
| PDB ID: 7R7S p47-bound p97-R155H mutant with ATPgammaS | ||||||
| Method | Electron microscopy | Resolution | 4.23 Å | Mutation | Yes | [4] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVHGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DAEVMNSLAV447 TMDDFRWALS457 QSNPSALRET467 VVEVPQVTWE477 DIGGLEDVKR 487 ELQELVQYPV497 EHPDKFLKFG507 MTPSKGVLFY517 GPPGCGKTLL527 AKAIANECQA 537 NFISIKGPEL547 LTMWFGANVR560 EIFDKARQAA570 PCVLFFDELD580 SIAKARGAAD 598 RVINQILTEM608 DGMSTKKNVF618 IIGATNRPDI628 IDPAILRPGR638 LDQLIYIPLP 648 DEKSRVAILK658 ANLRKSPVAK668 DVDLEFLAKM678 TNGFSGADLT688 EICQRACKLA 698 IRESIESEIR708 RERERDPVPE730 IRRDHFEEAM740 RFARRSVSDN750 DIRKYEMFAQ 760 TLQQSRGFGS770 FR
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:543 or .A:577 or .A:578 or .A:648 or .A:652 or .A:656 or .A:685 or .A:686 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.192
ILE206
3.873
GLY207
4.022
CYS209
4.771
PRO247
4.351
GLY248
3.207
THR249
2.858
GLY250
2.600
LYS251
2.399
THR252
2.838
LEU253
3.658
ASP304
4.002
ILE380
3.375
ILE383
3.656
HIS384
4.887
GLY408
3.450
ALA409
3.158
ALA412
4.066
ASP478
3.969
|
|||||
| PDB ID: 7VCU Human p97 double hexamer conformer I with D1-ATPgammaS and D2-ADP bound | ||||||
| Method | Electron microscopy | Resolution | 3.15 Å | Mutation | No | [5] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIRRE710 RERQTNPSAM720 EVEEDDPVPE 730 IRRDHFEEAM740 RFARRSVSDN750 DIRKYEMFAQ760 TLQQSRGFGS770 FRFPSGN |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .K:205 or .K:206 or .K:207 or .K:246 or .K:247 or .K:248 or .K:249 or .K:250 or .K:251 or .K:252 or .K:253 or .K:304 or .K:305 or .K:348 or .K:380 or .K:384 or .K:408 or .K:409 or .K:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
||||||
| PDB ID: 7VCT Human p97 single hexamer conformer III with D1-ATPgammaS and D2-ADP bound | ||||||
| Method | Electron microscopy | Resolution | 3.21 Å | Mutation | No | [5] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIRRE710 RERQTNPSAM720 EVEEDDPVPE 730 IRRDHFEEAM740 RFARRSVSDN750 DIRKYEMFAQ760 TLQQSRGFGS770 F |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.153
ILE206
4.052
GLY207
3.179
CYS209
4.829
PRO246
4.619
PRO247
3.686
GLY248
3.142
THR249
3.568
GLY250
3.220
LYS251
3.006
THR252
3.319
|
|||||
| PDB ID: 7VCV Human p97 single hexamer conformer I with ATPgammaS bound | ||||||
| Method | Electron microscopy | Resolution | 3.21 Å | Mutation | No | [5] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIRRER711 ERQTNPSAME 721 VEEDDPVPEI731 RRDHFEEAMR741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGFG |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:624 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.386
ILE206
3.932
GLY207
2.897
CYS209
4.531
PRO246
4.755
PRO247
4.080
GLY248
3.182
THR249
3.687
GLY250
3.105
LYS251
2.493
THR252
2.954
LEU253
3.508
GLU305
4.474
ASN348
3.388
ILE380
4.079
ILE383
4.760
HIS384
3.207
GLY408
3.718
ALA409
3.737
|
|||||
| PDB ID: 7VCX Human p97 single hexamer conformer II with ATPgammaS bound | ||||||
| Method | Electron microscopy | Resolution | 3.24 Å | Mutation | No | [5] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGGG594 GAADRVINQI604 LTEMDGMSTK614 KNVFIIGATN624 RPDIIDPAIL 634 RPGRLDQLIY644 IPLPDEKSRV654 AILKANLRKS664 PVAKDVDLEF674 LAKMTNGFSG 684 ADLTEICQRA694 CKLAIRESIE704 SEIRRERERQ714 TNPSAMEVEE724 DDPVPEIRRD 734 HFEEAMRFAR744 RSVSDNDIRK754 YEMFAQTLQQ764 SRGFGS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:624 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.374
ILE206
4.015
GLY207
3.115
CYS209
4.863
PRO246
4.922
PRO247
3.606
GLY248
2.852
THR249
3.478
GLY250
2.993
LYS251
2.985
THR252
3.227
LEU253
3.596
ASP304
4.861
GLU305
4.811
ASN348
3.463
ILE380
3.341
ILE383
4.603
HIS384
3.317
GLY408
3.908
ALA409
3.659
|
|||||
| PDB ID: 7VCS Human p97 double hexamer conformer II with ATPgammaS bound | ||||||
| Method | Electron microscopy | Resolution | 3.32 Å | Mutation | No | [5] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIRRER711 ERQTNPSAME 721 VEEDDPVPEI731 RRDHFEEAMR741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGFGSF 771 RFPSGN
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:578 or .A:624 or .A:656 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.228
ILE206
4.050
GLY207
3.427
CYS209
4.663
PRO246
4.419
PRO247
3.600
GLY248
2.616
THR249
2.690
GLY250
2.464
LYS251
3.102
THR252
3.109
LEU253
3.741
ASP304
4.909
GLU305
4.939
ASN348
4.164
ILE380
3.774
ILE383
4.851
HIS384
3.236
GLY408
3.985
ALA409
3.586
ALA412
4.913
|
|||||
| PDB ID: 7RL7 Cryo-EM structure of human p97-R155H mutant bound to ATPgS. | ||||||
| Method | Electron microscopy | Resolution | 3.00 Å | Mutation | Yes | [2] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVHGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIVPEI731 RRDHFEEAMR 741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:624 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.224
ILE206
3.914
GLY207
2.548
GLY208
4.769
CYS209
3.850
PRO246
4.970
PRO247
3.235
GLY248
2.964
THR249
3.099
GLY250
2.664
LYS251
2.641
THR252
2.239
LEU253
3.053
ASP304
4.425
GLU305
4.401
ASN348
2.649
ILE380
3.754
ILE383
4.536
HIS384
3.340
GLY408
3.423
ALA409
3.319
|
|||||
| PDB ID: 7RLA Cryo-EM structure of human p97-R191Q mutant bound to ATPgS. | ||||||
| Method | Electron microscopy | Resolution | 3.10 Å | Mutation | Yes | [2] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKQ191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIVPEI731 RRDHFEEAMR 741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:624 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.825
ILE206
3.935
GLY207
2.790
CYS209
4.524
PRO246
4.406
PRO247
4.013
GLY248
2.917
THR249
2.904
GLY250
2.444
LYS251
2.738
THR252
2.948
LEU253
3.328
GLU305
4.475
ASN348
4.879
ILE380
3.661
ILE383
4.621
HIS384
3.129
GLY408
3.681
ALA409
3.577
ALA412
4.897
|
|||||
| PDB ID: 7RLC Cryo-EM structure of human p97-A232E mutant bound to ATPgS. | ||||||
| Method | Electron microscopy | Resolution | 3.20 Å | Mutation | Yes | [2] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 EIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SALRETVVEV 471 PQVTWEDIGG481 LEDVKRELQE491 LVQYPVEHPD501 KFLKFGMTPS511 KGVLFYGPPG 521 CGKTLLAKAI531 ANECQANFIS541 IKGPELLTMW551 FGESEANVRE561 IFDKARQAAP 571 CVLFFDELDS581 IAKARGGNIG591 DGGGAADRVI601 NQILTEMDGM611 STKKNVFIIG 621 ATNRPDIIDP631 AILRPGRLDQ641 LIYIPLPDEK651 SRVAILKANL661 RKSPVAKDVD 671 LEFLAKMTNG681 FSGADLTEIC691 QRACKLAIRE701 SIESEIVPEI731 RRDHFEEAMR 741 FARRSVSDND751 IRKYEMFAQT761 LQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:577 or .A:624 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.447
ILE206
4.330
GLY207
3.383
PRO247
3.755
GLY248
3.182
THR249
3.204
GLY250
2.810
LYS251
2.863
THR252
2.862
LEU253
3.204
ASP304
4.649
GLU305
4.290
ASN348
3.646
ILE380
3.785
ILE383
3.542
HIS384
3.719
GLY408
3.552
ALA409
3.505
ALA412
4.771
|
|||||
| PDB ID: 5C1B p97-delta709-728 in complex with a UFD1-SHP peptide | ||||||
| Method | X-ray diffraction | Resolution | 3.08 Å | Mutation | No | [6] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGGA596 ADRVINQILT606 EMDGMSTKKN616 VFIIGATNRP626 DIIDPAILRP 636 GRLDQLIYIP646 LPDEKSRVAI656 LKANLRKSPV666 AKDVDLEFLA676 KMTNGFSGAD 686 LTEICQRACK696 LAIRESIESE706 IRPEIRRDHF736 EEAMRFARRS746 VSDNDIRKYE 756 MFAQTLQQSR766 GFGS
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:254 or .A:302 or .A:304 or .A:305 or .A:346 or .A:348 or .A:369 or .A:372 or .A:380 or .A:383 or .A:384 or .A:407 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:527 or .A:577 or .A:578 or .A:624 or .A:648 or .A:652 or .A:656 or .A:659 or .A:660 or .A:684 or .A:685 or .A:688; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
2.932
ILE206
2.990
GLY207
2.297
GLY208
4.307
CYS209
3.542
PRO246
4.256
PRO247
2.423
GLY248
2.319
THR249
2.394
GLY250
2.261
LYS251
1.753
THR252
2.061
LEU253
2.791
ILE254
4.774
PHE302
4.988
ASP304
4.061
GLU305
3.718
ALA346
4.367
ASN348
2.965
ILE369
4.757
PRO372
4.683
ILE380
2.496
ILE383
2.662
HIS384
2.323
VAL407
4.638
GLY408
2.763
ALA409
2.343
ALA412
2.415
ASP478
2.570
ILE479
2.365
GLY480
2.404
GLY481
4.850
LEU482
2.800
PRO519
4.533
PRO520
2.869
GLY521
1.857
CYS522
2.581
GLY523
2.398
LYS524
2.332
THR525
2.352
LEU526
2.224
LEU527
4.713
ASP577
4.815
GLU578
3.986
ASN624
2.215
PRO648
4.278
SER652
4.631
ILE656
2.539
ALA659
3.323
ASN660
3.034
GLY684
2.577
ALA685
2.602
THR688
2.061
|
|||||
| PDB ID: 5C18 p97-delta709-728 in complex with ATP-gamma-S | ||||||
| Method | X-ray diffraction | Resolution | 3.30 Å | Mutation | No | [7] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGAA597 DRVINQILTE607 MDGMSTKKNV617 FIIGATNRPD627 IIDPAILRPG 637 RLDQLIYIPL647 PDEKSRVAIL657 KANLRKSPVA667 KDVDLEFLAK677 MTNGFSGADL 687 TEICQRACKL697 AIRESIESEI707 RPEIRRDHFE737 EAMRFARRSV747 SDNDIRKYEM 757 FAQTLQQSRG767 FG
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:254 or .A:304 or .A:305 or .A:346 or .A:348 or .A:369 or .A:372 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412 or .A:478 or .A:479 or .A:480 or .A:481 or .A:482 or .A:519 or .A:520 or .A:521 or .A:522 or .A:523 or .A:524 or .A:525 or .A:526 or .A:527 or .A:577 or .A:578 or .A:624 or .A:648 or .A:656 or .A:659 or .A:660 or .A:683 or .A:684 or .A:685 or .A:688; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.156
ILE206
2.626
GLY207
2.456
GLY208
4.610
CYS209
2.946
PRO246
4.267
PRO247
2.740
GLY248
2.347
THR249
2.702
GLY250
2.469
LYS251
1.849
THR252
2.253
LEU253
2.287
ILE254
4.117
ASP304
4.617
GLU305
3.964
ALA346
4.558
ASN348
2.698
ILE369
4.989
PRO372
3.941
ILE380
2.644
ILE383
2.556
HIS384
2.225
GLY408
2.667
ALA409
2.299
ALA412
2.286
ASP478
2.511
ILE479
2.653
GLY480
2.310
GLY481
4.406
LEU482
3.503
PRO519
4.337
PRO520
3.407
GLY521
1.768
CYS522
2.618
GLY523
2.322
LYS524
2.375
THR525
2.216
LEU526
2.149
LEU527
4.598
ASP577
4.689
GLU578
3.999
ASN624
1.803
PRO648
4.733
ILE656
2.621
ALA659
3.286
ASN660
3.537
SER683
4.928
GLY684
2.676
ALA685
2.728
THR688
2.528
|
|||||
| PDB ID: 7RLJ Cryo-EM structure of human p97 bound to CB-5083 and ATPgS. | ||||||
| Method | Electron microscopy | Resolution | 3.80 Å | Mutation | No | [2] |
| PDB Sequence |
NRPNRLIVDE
30 AINEDNSVVS40 LSQPKMDELQ50 LFRGDTVLLK60 GKKRREAVCI70 VLSDDTCSDE 80 KIRMNRVVRN90 NLRVRLGDVI100 SIQPCPDVKY110 GKRIHVLPID120 DTVEGITGNL 130 FEVYLKPYFL140 EAYRPIRKGD150 IFLVRGGMRA160 VEFKVVETDP170 SPYCIVAPDT 180 VIHCEGEPIK190 REDEEESLNE200 VGYDDIGGCR210 KQLAQIKEMV220 ELPLRHPALF 230 KAIGVKPPRG240 ILLYGPPGTG250 KTLIARAVAN260 ETGAFFFLIN270 GPEIMSKLAG 280 ESESNLRKAF290 EEAEKNAPAI300 IFIDELDAIA310 PKREKTHGEV320 ERRIVSQLLT 330 LMDGLKQRAH340 VIVMAATNRP350 NSIDPALRRF360 GRFDREVDIG370 IPDATGRLEI 380 LQIHTKNMKL390 ADDVDLEQVA400 NETHGHVGAD410 LAALCSEAAL420 QAIRKKMDLI 430 DLEDETIDAE440 VMNSLAVTMD450 DFRWALSQSN460 PSALRETVVE470 VPQVTWEDIG 480 GLEDVKRELQ490 ELVQYPVEHP500 DKFLKFGMTP510 SKGVLFYGPP520 GCGKTLLAKA 530 IANECQANFI540 SIKGPELLTM550 WFGESEANVR560 EIFDKARQAA570 PCVLFFDELD 580 SIAKARGGNI590 GDGGGAADRV600 INQILTEMDG610 MSTKKNVFII620 GATNRPDIID 630 PAILRPGRLD640 QLIYIPLPDE650 KSRVAILKAN660 LRKSPVAKDV670 DLEFLAKMTN 680 GFSGADLTEI690 CQRACKLAIR700 ESIESEIVPE730 IRRDHFEEAM740 RFARRSVSDN 750 DIRKYEMFAQ760 TLQQSRGF
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:304 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.997
ILE206
4.316
GLY207
3.585
CYS209
4.830
PRO246
4.434
PRO247
3.849
GLY248
2.810
THR249
2.749
GLY250
2.360
LYS251
2.596
THR252
3.134
|
|||||
| PDB ID: 4KO8 Structure of p97 N-D1 R155H mutant in complex with ATPgS | ||||||
| Method | X-ray diffraction | Resolution | 1.98 Å | Mutation | Yes | [8] |
| PDB Sequence |
TAILKQKNRP
23 NRLIVDEAIN33 EDNSVVSLSQ43 PKMDELQLFR53 GDTVLLKGKK63 RREAVCIVLS 73 DDTCSDEKIR83 MNRVVRNNLR93 VRLGDVISIQ103 PCPDVKYGKR113 IHVLPIDDTV 123 EGITGNLFEV133 YLKPYFLEAY143 RPIRKGDIFL153 VHGGMRAVEF163 KVVETDPSPY 173 CIVAPDTVIH183 CEGEPIKRED193 EEESLNEVGY203 DDIGGCRKQL213 AQIKEMVELP 223 LRHPALFKAI233 GVKPPRGILL243 YGPPGTGKTL253 IARAVANETG263 AFFFLINGPE 273 IMSKLAGESE283 SNLRKAFEEA293 EKNAPAIIFI303 DELDAIAPKR313 EKTHGEVERR 323 IVSQLLTLMD333 GLKQRAHVIV343 MAATNRPNSI353 DPALRRFGRF363 DREVDIGIPD 373 ATGRLEILQI383 HTKNMKLADD393 VDLEQVANET403 HGHVGADLAA413 LCSEAALQAI 423 RKKMDLIDLE433 DETIDAEVMN443 SLAVTMDDFR453 WALSQSNPSA463 LRETVV |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:245 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.290
ILE206
3.731
GLY207
2.785
CYS209
3.740
GLY245
4.950
PRO246
4.281
PRO247
3.754
GLY248
2.883
THR249
3.096
GLY250
2.858
LYS251
2.834
|
|||||
| PDB ID: 3HU3 Structure of p97 N-D1 R155H mutant in complex with ATPgS | ||||||
| Method | X-ray diffraction | Resolution | 2.20 Å | Mutation | Yes | [9] |
| PDB Sequence |
LKQKNRPNRL
26 IVDEAINEDN36 SVVSLSQPKM46 DELQLFRGDT56 VLLKGKKRRE66 AVCIVLSDDT 76 CSDEKIRMNR86 VVRNNLRVRL96 GDVISIQPCP106 DVKYGKRIHV116 LPIDDTVEGI 126 TGNLFEVYLK136 PYFLEAYRPI146 RKGDIFLVHG156 GMRAVEFKVV166 ETDPSPYCIV 176 APDTVIHCEG186 EPIKREDEEE196 SLNEVGYDDI206 GGCRKQLAQI216 KEMVELPLRH 226 PALFKAIGVK236 PPRGILLYGP246 PGTGKTLIAR256 AVANETGAFF266 FLINGPEIMS 276 KLAGESESNL286 RKAFEEAEKN296 APAIIFIDEL306 DAIAPKREKT316 HGEVERRIVS 326 QLLTLMDGLK336 QRAHVIVMAA346 TNRPNSIDPA356 LRRFGRFDRE366 VDIGIPDATG 376 RLEILQIHTK386 NMKLADDVDL396 EQVANETHGH406 VGADLAALCS416 EAALQAIRKK 426 MDLIDLEDET436 IDAEVMNSLA446 VTMDDFRWAL456 SQSNPSALRE466 TVV |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:245 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.386
ILE206
3.740
GLY207
2.840
CYS209
3.814
GLY245
4.857
PRO246
4.413
PRO247
3.757
GLY248
2.989
THR249
3.047
GLY250
2.810
LYS251
2.925
|
|||||
| PDB ID: 4KLN Structure of p97 N-D1 A232E mutant in complex with ATPgS | ||||||
| Method | X-ray diffraction | Resolution | 2.62 Å | Mutation | Yes | [8] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 EIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 S |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:245 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:347 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.593
ILE206
3.734
GLY207
3.096
CYS209
4.117
GLY245
4.940
PRO247
3.949
GLY248
2.916
THR249
2.819
GLY250
2.946
LYS251
2.699
THR252
2.922
|
|||||
| PDB ID: 3HU1 Structure of p97 N-D1 R95G mutant in complex with ATPgS | ||||||
| Method | X-ray diffraction | Resolution | 2.81 Å | Mutation | Yes | [9] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNRVVRNN91 LRVGLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 S |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:209 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:347 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.399
ILE206
3.411
GLY207
2.835
CYS209
4.376
PRO246
4.396
PRO247
3.579
GLY248
2.905
THR249
2.948
GLY250
2.937
LYS251
2.816
THR252
2.761
|
|||||
| PDB ID: 3HU2 Structure of p97 N-D1 R86A mutant in complex with ATPgS | ||||||
| Method | X-ray diffraction | Resolution | 2.85 Å | Mutation | Yes | [9] |
| PDB Sequence |
LSTAILKQKN
21 RPNRLIVDEA31 INEDNSVVSL41 SQPKMDELQL51 FRGDTVLLKG61 KKRREAVCIV 71 LSDDTCSDEK81 IRMNAVVRNN91 LRVRLGDVIS101 IQPCPDVKYG111 KRIHVLPIDD 121 TVEGITGNLF131 EVYLKPYFLE141 AYRPIRKGDI151 FLVRGGMRAV161 EFKVVETDPS 171 PYCIVAPDTV181 IHCEGEPIKR191 EDEEESLNEV201 GYDDIGGCRK211 QLAQIKEMVE 221 LPLRHPALFK231 AIGVKPPRGI241 LLYGPPGTGK251 TLIARAVANE261 TGAFFFLING 271 PEIMSKLAGE281 SESNLRKAFE291 EAEKNAPAII301 FIDELDAIAP311 KREKTHGEVE 321 RRIVSQLLTL331 MDGLKQRAHV341 IVMAATNRPN351 SIDPALRRFG361 RFDREVDIGI 371 PDATGRLEIL381 QIHTKNMKLA391 DDVDLEQVAN401 ETHGHVGADL411 AALCSEAALQ 421 AIRKKMDLID431 LEDETIDAEV441 MNSLAVTMDD451 FRWALSQSNP461 SA |
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .AGS or .AGS2 or .AGS3 or :3AGS;style chemicals stick;color identity;select .A:205 or .A:206 or .A:207 or .A:208 or .A:209 or .A:245 or .A:246 or .A:247 or .A:248 or .A:249 or .A:250 or .A:251 or .A:252 or .A:253 or .A:305 or .A:347 or .A:348 or .A:380 or .A:383 or .A:384 or .A:408 or .A:409 or .A:412; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
ASP205
3.514
ILE206
3.399
GLY207
2.686
GLY208
4.969
CYS209
3.958
GLY245
4.968
PRO246
4.409
PRO247
3.395
GLY248
2.757
THR249
2.959
GLY250
2.805
LYS251
2.682
|
|||||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Seesaw conformations of Npl4 in the human p97 complex and the inhibitory mechanism of a disulfiram derivative. Nat Commun. 2021 Jan 5;12(1):121. | ||||
| REF 2 | AAA+ ATPase p97/VCP mutants and inhibitor binding disrupt inter-domain coupling and subsequent allosteric activation. J Biol Chem. 2021 Oct;297(4):101187. | ||||
| REF 3 | 2.3 ? resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. Science. 2016 Feb 19;351(6275):871-5. | ||||
| REF 4 | Structural and Functional Analysis of Disease-Linked p97 ATPase Mutant Complexes. Int J Mol Sci. 2021 Jul 28;22(15):8079. | ||||
| REF 5 | Cryo-EM structures of human p97 double hexamer capture potentiated ATPase-competent state. Cell Discov. 2022 Feb 22;8(1):19. | ||||
| REF 6 | Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure. 2016 Jan 5;24(1):140-147. | ||||
| REF 7 | Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97. Structure. 2016 Jan 5;24(1):127-139. | ||||
| REF 8 | Altered intersubunit communication is the molecular basis for functional defects of pathogenic p97 mutants. J Biol Chem. 2013 Dec 20;288(51):36624-35. | ||||
| REF 9 | A novel ATP-dependent conformation in p97 N-D1 fragment revealed by crystal structures of disease-related mutants. EMBO J. 2010 Jul 7;29(13):2217-29. | ||||
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