Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T26463 | Target Info | |||
| Target Name | DNA polymerase alpha catalytic p180 (POLA1) | ||||
| Synonyms | POLA; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha catalytic subunit | ||||
| Target Type | Discontinued Target | ||||
| Gene Name | POLA1 | ||||
| Biochemical Class | Kinase | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | 2'-Deoxycytidine-5'-triphosphate | Ligand Info | |||
| Canonical SMILES | C1C(C(OC1N2C=CC(=NC2=O)N)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O | ||||
| InChI | 1S/C9H16N3O13P3/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17)/t5-,6+,8+/m0/s1 | ||||
| InChIKey | RGWHQCVHVJXOKC-SHYZEUOFSA-N | ||||
| PubChem Compound ID | 65091 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 4QCL Crystal structure of the catalytic core of human DNA polymerase alpha in ternary complex with an RNA-primed DNA template and dCTP | ||||||
| Method | X-ray diffraction | Resolution | 2.20 Å | Mutation | Yes | [1] |
| PDB Sequence |
EQVFHFYWLD
347 AYEDQYNQPG357 VVFLFGKVWI367 ESAETHVSCC377 VMVKNIERTL387 YFLPREMKID 397 LNTGKETGTP407 ISMKDVYEEF417 DEKIATKYKI427 MKFKSKPVEK437 NYAFEIPDVP 447 EKSEYLEVKY457 SAEMPQLPQD467 LKGETFSHVF477 GTNTSSLELF487 LMNRKIKGPC 497 WLEVKSPQLL507 NQPVSWCKAE517 AMALKPDLVN527 VIKDVSPPPL537 VVMAFSMKTM 547 QNAKNHQNEI557 IAMAALVHHS567 FALDKAAPKP577 PFQSHFCVVS587 KPKDCIFPYA 597 FKEVIEKKNV607 KVEVAATERT617 LLGFFLAKVH627 KIDPDIIVGH637 NIYGFELEVL 647 LQRINVCKAP657 HWSKIGRLKR667 SNMPKLGFGE681 RNATCGRMIC691 DVEISAKELI 701 RCKSYHLSEL711 VQQILKTERV721 VIPMENIQNM731 YSESSQLLYL741 LEHTWKDAKF 751 ILQIMCELNV761 LPLALQITNI771 AGNIMSRTLM781 GGRSERNEFL791 LLHAFYENNY 801 IVPDKQIRKK836 AAYAGGLVLD846 PKVGFYDKFI856 LLLDFNSLYP866 SIIQEFNICF 876 TTVQRVEQIP899 ELPDPSLEMG909 ILPREIRKLV919 ERRKQVKQLM929 KQQDLNPDLI 939 LQYDIRQKAL949 KLTANSMYGC959 LGFSYSRFYA969 KPLAALVTYK979 GREILMHTKE 989 MVQKMNLEVI999 YGDTDSIMIN1009 TNSTNLEEVF1019 KLGNKVKSEV1029 NKLYKLLEID 1039 IDGVFKSLLL1049 LKKKKYAALV1059 VEPTSDGNYV1069 TKQELKGLDI1079 VRRDWCDLAK 1089 DTGNFVIGQI1099 LSDQSRDTIV1109 ENIQKRLIEI1119 GENVLNGSVP1129 VSQFEINKAL 1139 TKDPQDYPDK1149 KSLPHVHVAL1159 WINSQGGRKV1169 KAGDTVSYVI1179 CQDGSNLTAS 1189 QRAYAPEQLQ1199 KQDNLTIDTQ1209 YYLAQQIHPV1219 VARICEPIDG1229 IDAVLIATWL 1239 GLDPT
|
|||||
|
|
||||||
| PDB ID: 7N2M Crystal structure of DNA polymerase alpha catalytic core in complex with dCTP and template/primer having T-C mismatch at the post-insertion site | ||||||
| Method | X-ray diffraction | Resolution | 2.90 Å | Mutation | No | [2] |
| PDB Sequence |
EQVFHFYWLD
347 AYEDQYNQPG357 VVFLFGKVWI367 ESAETHVSCC377 VMVKNIERTL387 YFLPREMKID 397 LNTGKETGTP407 ISMKDVYEEF417 DEKIATKYKI427 MKFKSKPVEK437 NYAFEIPDVP 447 EKSEYLEVKY457 SAEMPQLPQD467 LKGETFSHVF477 GTNTSSLELF487 LMNRKIKGPC 497 WLEVKSPQLL507 NQPVSWCKAE517 AMALKPDLVN527 VIKDVSPPPL537 VVMAFSMKTM 547 QNAKNHQNEI557 IAMAALVHHS567 FALDKAAPKP577 PFQSHFCVVS587 KPKDCIFPYA 597 FKEVIEKKNV607 KVEVAATERT617 LLGFFLAKVH627 KIDPDIIVGH637 NIYGFELEVL 647 LQRINVCKAP657 HWSKIGRLKR667 SNMPKLGFGE681 RNATCGRMIC691 DVEISAKELI 701 RCKSYHLSEL711 VQQILKTERV721 VIPMENIQNM731 YSESSQLLYL741 LEHTWKDAKF 751 ILQIMCELNV761 LPLALQITNI771 AGNIMSRTLM781 GGRSERNEFL791 LLHAFYENNY 801 IVPDKQIRKK836 AAYAGGLVLD846 PKVGFYDKFI856 LLLDFNSLYP866 SIIQEFNICF 876 TTVQRVEQIP899 ELPDPSLEMG909 ILPREIRKLV919 ERRKQVKQLM929 KQQDLNPDLI 939 LQYDIRQKAL949 KLTANSMYGC959 LGFSYSRFYA969 KPLAALVTYK979 GREILMHTKE 989 MVQKMNLEVI999 YGDTDSIMIN1009 TNSTNLEEVF1019 KLGNKVKSEV1029 NKLYKLLEID 1039 IDGVFKSLLL1049 LKKKKYAALV1059 VEPTSDGNYV1069 TKQELKGLDI1079 VRRDWCDLAK 1089 DTGNFVIGQI1099 LSDQSRDTIV1109 ENIQKRLIEI1119 GENVLNGSVP1129 VSQFEINKAL 1139 TKDPQDYPDK1149 KSLPHVHVAL1159 WINSQGGRKV1169 KAGDTVSYVI1179 CQDGSNLTAS 1189 QRAYAPEQLQ1199 KQDNLTIDTQ1209 YYLAQQIHPV1219 VARICEPIDG1229 IDAVLIATWL 1239 GLDPT
|
|||||
|
|
||||||
| PDB ID: 6AS7 CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF HUMAN DNA POLYMERASE ALPHA IN TERNARY COMPLEX WITH AN DNA-PRIMED DNA TEMPLATE AND DCTP | ||||||
| Method | X-ray diffraction | Resolution | 2.95 Å | Mutation | Yes | [3] |
| PDB Sequence |
EQVFHFYWLD
347 AYEDQYNQPG357 VVFLFGKVWI367 ESAETHVSCC377 VMVKNIERTL387 YFLPREMKID 397 LNTGKETGTP407 ISMKDVYEEF417 DEKIATKYKI427 MKFKSKPVEK437 NYAFEIPDVP 447 EKSEYLEVKY457 SAEMPQLPQD467 LKGETFSHVF477 GTNTSSLELF487 LMNRKIKGPC 497 WLEVKSPQLL507 NQPVSWCKAE517 AMALKPDLVN527 VIKDVSPPPL537 VVMAFSMKTM 547 QNAKNHQNEI557 IAMAALVHHS567 FALDKAAPKP577 PFQSHFCVVS587 KPKDCIFPYA 597 FKEVIEKKNV607 KVEVAATERT617 LLGFFLAKVH627 KIDPDIIVGH637 NIYGFELEVL 647 LQRINVCKAP657 HWSKIGRLKR667 SNMPKLGFGE681 RNATCGRMIC691 DVEISAKELI 701 RCKSYHLSEL711 VQQILKTERV721 VIPMENIQNM731 YSESSQLLYL741 LEHTWKDAKF 751 ILQIMCELNV761 LPLALQITNI771 AGNIMSRTLM781 GGRSERNEFL791 LLHAFYENNY 801 IVPDKQIFRK835 KAAYAGGLVL845 DPKVGFYDKF855 ILLLDFNSLY865 PSIIQEFNIC 875 FTTVQRVEQI898 PELPDPSLEM908 GILPREIRKL918 VERRKQVKQL928 MKQQDLNPDL 938 ILQYDIRQKA948 LKLTANSMYG958 CLGFSYSRFY968 AKPLAALVTY978 KGREILMHTK 988 EMVQKMNLEV998 IYGDTDSIMI1008 NTNSTNLEEV1018 FKLGNKVKSE1028 VNKLYKLLEI 1038 DIDGVFKSLL1048 LLKKKKYAAL1058 VVEPTSDGNY1068 VTKQELKGLD1078 IVRRDWCDLA 1088 KDTGNFVIGQ1098 ILSDQSRDTI1108 VENIQKRLIE1118 IGENVLNGSV1128 PVSQFEINKA 1138 LTKDPQDYPD1148 KKSLPHVHVA1158 LWINSQGGRK1168 VKAGDTVSYV1178 ICQDGSNLTA 1188 SQRAYAPEQL1198 QKQDNLTIDT1208 QYYLAQQIHP1218 VVARICEPID1228 GIDAVLIATW 1238 LGLDPTQFRV1248
|
|||||
|
|
||||||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Activity and fidelity of human DNA polymerase alpha depend on primer structure | ||||
| REF 2 | Structural and functional insight into mismatch extension by human DNA polymerase Alpha. Proc Natl Acad Sci U S A. 2022 Apr 26;119(17):e2111744119. | ||||
| REF 3 | Activity and fidelity of human DNA polymerase Alpha depend on primer structure. J Biol Chem. 2018 May 4;293(18):6824-6843. | ||||
If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.