Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T55703 | Target Info | |||
| Target Name | Plasma retinol-binding protein (RBP4) | ||||
| Synonyms | Retinol-binding protein 4; RBP4; RBP; Plasma retinol-binding protein(1-176); PRBP | ||||
| Target Type | Clinical trial Target | ||||
| Gene Name | RBP4 | ||||
| Biochemical Class | Calycin family | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | Vitamin A | Ligand Info | |||
| Canonical SMILES | CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CCO)C)C | ||||
| InChI | 1S/C20H30O/c1-16(8-6-9-17(2)13-15-21)11-12-19-18(3)10-7-14-20(19,4)5/h6,8-9,11-13,21H,7,10,14-15H2,1-5H3/b9-6+,12-11+,16-8+,17-13+ | ||||
| InChIKey | FPIPGXGPPPQFEQ-OVSJKPMPSA-N | ||||
| PubChem Compound ID | 445354 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 5NU7 Structure of human holo plasma RBP4 | ||||||
| Method | X-ray diffraction | Resolution | 1.50 Å | Mutation | No | [1] |
| PDB Sequence |
ERDCRVSSFR
10 VKENFDKARF20 SGTWYAMAKK30 DPEGLFLQDN40 IVAEFSVDET50 GQMSATAKGR 60 VRLLNNWDVC70 ADMVGTFTDT80 EDPAKFKMKY90 WGVASFLQKG100 NDDHWIVDTD 110 YDTYAVQYSC120 RLLNLDGTCA130 DSYSFVFSRD140 PNGLPPEAQK150 IVRQRQEELC 160 LARQYRLIVH170 NGYCD
|
|||||
|
|
LEU35
3.581
PHE36
3.594
LEU37
3.977
ALA43
4.024
PHE45
4.068
ALA55
3.927
THR56
4.429
ALA57
4.095
VAL61
3.720
LEU63
4.470
ALA71
4.657
MET73
3.654
VAL74
4.335
GLY75
4.536
|
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| PDB ID: 1RBP CRYSTALLOGRAPHIC REFINEMENT OF HUMAN SERUM RETINOL BINDING PROTEIN AT 2 ANGSTROMS RESOLUTION | ||||||
| Method | X-ray diffraction | Resolution | 2.00 Å | Mutation | No | [2] |
| PDB Sequence |
ERDCRVSSFR
10 VKENFDKARF20 SGTWYAMAKK30 DPEGLFLQDN40 IVAEFSVDET50 GQMSATAKGR 60 VRLLNNWDVC70 ADMVGTFTDT80 EDPAKFKMKY90 WGVASFLQKG100 NDDHWIVDTD 110 YDTYAVQYSC120 RLLNLDGTCA130 DSYSFVFSRD140 PNGLPPEAQK150 IVRQRQEELC 160 LARQYRLIVH170 NGYCD
|
|||||
|
|
LEU35
3.248
PHE36
3.582
LEU37
3.927
ILE41
4.820
ALA43
3.846
PHE45
3.951
ALA55
3.583
THR56
4.405
ALA57
3.887
VAL61
3.694
LEU63
3.186
MET73
3.893
VAL74
4.010
GLY75
3.891
|
|||||
| PDB ID: 1BRP CRYSTAL STRUCTURE OF THE TRIGONAL FORM OF HUMAN PLASMA RETINOL-BINDING PROTEIN AT 2.5 ANGSTROMS RESOLUTION | ||||||
| Method | X-ray diffraction | Resolution | 2.50 Å | Mutation | No | [3] |
| PDB Sequence |
ERDCRVSSFR
10 VKENFDKARF20 SGTWYAMAKK30 DPEGLFLQDN40 IVAEFSVDET50 GQMSATAKGR 60 VRLLNNWDVC70 ADMVGTFTDT80 EDPAKFKMKY90 WGVASFLQKG100 NDDHWIVDTD 110 YDTYAVQYSC120 RLLNLDGTCA130 DSYSFVFSRD140 PNGLPPEAQK150 IVRQRQEELC 160 LARQYRLIVH170 NGYCD
|
|||||
|
|
LEU35
3.853
PHE36
3.419
LEU37
3.463
ILE41
4.645
ALA43
4.474
PHE45
3.828
ALA55
4.291
THR56
4.605
ALA57
3.621
VAL61
3.438
LEU63
4.456
ALA71
4.672
MET73
3.886
VAL74
4.202
|
|||||
| PDB ID: 1QAB The structure of human retinol binding protein with its carrier protein transthyretin reveals interaction with the carboxy terminus of RBP | ||||||
| Method | X-ray diffraction | Resolution | 3.20 Å | Mutation | No | [4] |
| PDB Sequence |
CAVSSFRVKE
13 NFDKARFSGT23 WYAMAKKDPE33 GLFLQDNIVA43 EFSVDETGQM53 SATAKGRVRL 63 LNNWDVCADM73 VGTFTDTEDP83 AKFKMKYWGV93 ASFLQKGNDD103 HWIVDTDYDT 113 YAVQYSCRLL123 NLDGTCADSY133 SFVFSRDPNG143 LPPEAQKIVA153 QRQEELCLAA 163 QYRLIVHNGY173 CDGRSERNLL183
|
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|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .RTL or .RTL2 or .RTL3 or :3RTL;style chemicals stick;color identity;select .E:35 or .E:36 or .E:37 or .E:41 or .E:45 or .E:55 or .E:56 or .E:57 or .E:61 or .E:63 or .E:71 or .E:73 or .E:74 or .E:75 or .E:77 or .E:88 or .E:90 or .E:96 or .E:97 or .E:98 or .E:104 or .E:117 or .E:133 or .E:135; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
LEU35
4.411
PHE36
2.775
LEU37
3.702
ILE41
4.729
PHE45
3.796
ALA55
4.086
THR56
4.689
ALA57
3.544
VAL61
3.861
LEU63
4.349
ALA71
4.628
MET73
3.628
|
|||||
| PDB ID: 3BSZ Crystal structure of the transthyretin-retinol binding protein-Fab complex | ||||||
| Method | X-ray diffraction | Resolution | 3.38 Å | Mutation | No | [5] |
| PDB Sequence |
ERDCRVSSFR
10 VKENFDKARF20 SGTWYAMAKK30 DPEGLFLQDN40 IVAEFSVDET50 GQMSATAKGR 60 VRLLNNWDVC70 ADMVGTFTDT80 EDPAKFKMKY90 WGVASFLQKG100 NDDHWIVDTD 110 YDTYAVQYSC120 RLLNLDGTCA130 DSYSFVFSRD140 PNGLPPEAQK150 IVRQRQEELC 160 LARQYRLIVH170 NGYC
|
|||||
|
Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .RTL or .RTL2 or .RTL3 or :3RTL;style chemicals stick;color identity;select .E:35 or .E:36 or .E:37 or .E:41 or .E:43 or .E:45 or .E:55 or .E:56 or .E:57 or .E:61 or .E:63 or .E:71 or .E:73 or .E:74 or .E:75 or .E:77 or .E:88 or .E:90 or .E:96 or .E:97 or .E:98 or .E:102 or .E:104 or .E:117 or .E:121 or .E:133 or .E:135 or .E:137; color #00ffc7; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
|
LEU35
3.877
PHE36
3.622
LEU37
3.554
ILE41
4.906
ALA43
3.786
PHE45
4.146
ALA55
4.055
THR56
4.836
ALA57
3.882
VAL61
3.855
LEU63
4.243
ALA71
4.964
MET73
3.577
VAL74
4.357
|
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| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Human plasma retinol-binding protein (RBP4) is also a fatty acid-binding protein. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Apr;1863(4):458-466. | ||||
| REF 2 | Crystallographic refinement of human serum retinol binding protein at 2A resolution. Proteins. 1990;8(1):44-61. | ||||
| REF 3 | Crystal structure of the trigonal form of human plasma retinol-binding protein at 2.5 A resolution. J Mol Biol. 1993 Mar 20;230(2):613-24. | ||||
| REF 4 | The structure of human retinol-binding protein (RBP) with its carrier protein transthyretin reveals an interaction with the carboxy terminus of RBP. Biochemistry. 1999 Mar 2;38(9):2647-53. | ||||
| REF 5 | Structural and mutational analyses of protein-protein interactions between transthyretin and retinol-binding protein. FEBS J. 2008 Dec;275(23):5841-54. | ||||
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