Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T94255 | Target Info | |||
| Target Name | Long transient receptor potential channel 4 (TRPM4) | ||||
| Synonyms | hTRPM4; Transient receptor potential cation channel subfamily M member 4; Melastatin-4; LTrpC4; LTrpC-4; Calcium-activated non-selective cation channel 1 | ||||
| Target Type | Clinical trial Target | ||||
| Gene Name | TRPM4 | ||||
| Biochemical Class | Transient receptor potential catioin channel | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | Cholesterol hemisuccinate | Ligand Info | |||
| Canonical SMILES | CC(C)CCCC(C)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)OC(=O)CCC(=O)O)C)C | ||||
| InChI | 1S/C31H50O4/c1-20(2)7-6-8-21(3)25-11-12-26-24-10-9-22-19-23(35-29(34)14-13-28(32)33)15-17-30(22,4)27(24)16-18-31(25,26)5/h9,20-21,23-27H,6-8,10-19H2,1-5H3,(H,32,33)/t21-,23+,24+,25-,26+,27+,30+,31-/m1/s1 | ||||
| InChIKey | WLNARFZDISHUGS-MIXBDBMTSA-N | ||||
| PubChem Compound ID | 65082 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 6BQV Human TRPM4 ion channel in lipid nanodiscs in a calcium-bound state | ||||||
| Method | Electron microscopy | Resolution | 3.10 Å | Mutation | No | [1] |
| PDB Sequence |
TGAGRHSNFL
91 RLSDRTDPAA101 VYSLVTRTWG111 FRAPNLVVSV121 LGGSGGPVLQ131 TWLQDLLRRG 141 LVRAAQSTGA151 WIVTGGLHTG161 IGRHVGVAVR171 DHQMASTGGT181 KVVAMGVAPW 191 GVVRNRDTLI201 NPKGSFPARY211 RWVQFPLDYN228 YSAFFLVDDL243 GGENRFRLRL 253 ESYISQQKTG263 VGGTGIDIPV273 LLLLIDGDEK283 MLTRIENATQ293 AQLPCLLVAG 303 SGGAADCLAE313 TLEDTLGEAR332 DRIRRFFPKG342 DLEVLQAQVE352 RIMTRKELLT 362 VYSSEDGSEE372 FETIVLKALV382 KACGEASAYL394 DELRLAVAWN404 RVDIAQSELF 414 RGDIQWRSFH424 LEASLMDALL434 NDRPEFVRLL444 ISHGLSLGHF454 LTPMRLAQLY 464 SAAPSNSLIR474 NLLDQASVGH505 VLRMLLGPWS560 DLLLWALLLN570 RAQMAMYFWE 580 MGSNAVSSAL590 GACLLLRVMA600 RLEPDAEEAA610 RRKDLAFKFE620 GMGVDLFGEC 630 YRSSEVRAAR640 LLLRRCPLWG650 DATCLQLAMQ660 ADARAFFAQD670 GVQSLLTQKW 680 WGDMASTTPI690 WALVLAFFCP700 PLIYTRLITF710 RRCLRRWFHF775 WGAPVTIFMG 785 NVVSYLLFLL795 LFSRVLLVDF805 QPAPPGSLEL815 LLYFWAFTLL825 CEELRQGLSG 835 SGGPGPGHAS851 LSQRLRLYLA861 DSWNQCDLVA871 LTCFLLGVGC881 RLTPGLYHLG 891 RTVLCIDFMV901 FTVRLLHIFT911 VNKQLGPKIV921 IVSKMMKDVF931 FFLFFLGVWL 941 VAYGVATEGL951 LRPRDSDFPS961 ILRRVFYRPY971 LQIFGQIPQE981 DMDVALMEHS 991 NCSSEPGFWA1001 HPPGAQAGTC1011 VSQYANWLVV1021 LLLVIFLLVA1031 NILLVNLLIA 1041 MFSYTFGKVQ1051 GNSDLYWKAQ1061 RYRLIREFHS1071 RPALAPPFIV1081 ISHLRLLLRQ 1091 LCREHFRVYL1113 SKEAERKLLT1123 WESVHKENFL1133 LARARDKRES1143 DSERLKRTSQ 1153 KVDLALKQLG1163 HIREYEQRLK1173 VL
|
|||||
|
|
TRP680
3.324
ILE690
3.678
LEU693
4.817
VAL694
3.642
PHE698
4.100
VAL780
4.018
PHE783
3.172
MET784
3.926
VAL787
3.748
VAL788
3.682
LEU791
3.380
LEU792
4.318
LEU795
3.649
TRP864
4.008
VAL904
4.245
LEU907
3.655
HIS908
3.590
PHE910
4.110
VAL912
4.500
|
|||||
| PDB ID: 6BQR Human TRPM4 ion channel in lipid nanodiscs in a calcium-free state | ||||||
| Method | Electron microscopy | Resolution | 3.20 Å | Mutation | No | [1] |
| PDB Sequence |
YGELDFTGAG
84 RHSNFLRLSD95 RTDPAAVYSL105 VTRTWGFRAP115 NLVVSVLGGS125 GGPVLQTWLQ 135 DLLRRGLVRA145 AQSTGAWIVT155 GGLHTGIGRH165 VGVAVRDHQM175 ASTGGTKVVA 185 MGVAPWGVVR195 NRDTLINPKG205 SFPARYRWRG215 DPEDGVQFPL225 DYNYSAFFLV 235 DDGTHGCLGG245 ENRFRLRLES255 YISQQKTGVG265 GTGIDIPVLL275 LLIDGDEKML 285 TRIENATQAQ295 LPCLLVAGSG305 GAADCLAETL315 EDTLAPGSGG325 ARQGEARDRI 335 RRFFPKGDLE345 VLQAQVERIM355 TRKELLTVYS365 SEDGSEEFET375 IVLKALVKAC 385 GEASAYLDEL397 RLAVAWNRVD407 IAQSELFRGD417 IQWRSFHLEA427 SLMDALLNDR 437 PEFVRLLISH447 GLSLGHFLTP457 MRLAQLYSAA467 PSNSLIRNLL477 DQASHSVGHV 506 LRMLLGPWSD561 LLLWALLLNR571 AQMAMYFWEM581 GSNAVSSALG591 ACLLLRVMAR 601 LEPDAEEAAR611 RKDLAFKFEG621 MGVDLFGECY631 RSSEVRAARL641 LLRRCPLWGD 651 ATCLQLAMQA661 DARAFFAQDG671 VQSLLTQKWW681 GDMASTTPIW691 ALVLAFFCPP 701 LIYTRLITFR711 RRCLRRWFHF775 WGAPVTIFMG785 NVVSYLLFLL795 LFSRVLLVDF 805 QPAPPGSLEL815 LLYFWAFTLL825 CEELRQGLSG835 SLSQRLRLYL860 ADSWNQCDLV 870 ALTCFLLGVG880 CRLTPGLYHL890 GRTVLCIDFM900 VFTVRLLHIF910 TVNKQLGPKI 920 VIVSKMMKDV930 FFFLFFLGVW940 LVAYGVATEG950 LLRPRDSDFP960 SILRRVFYRP 970 YLQIFGQIPQ980 EDMDVALMEH990 SNCSSEPGFW1000 AHPPGAQAGT1010 CVSQYANWLV 1020 VLLLVIFLLV1030 ANILLVNLLI1040 AMFSYTFGKV1050 QGNSDLYWKA1060 QRYRLIREFH 1070 SRPALAPPFI1080 VISHLRLLLR1090 QLCRRPRHFR1110 VYLSKEAERK1120 LLTWESVHKE 1130 NFLLARARDK1140 RESDSERLKR1150 TSQKVDLALK1160 QLGHIREY
|
|||||
|
|
TRP680
2.790
ILE690
3.551
LEU693
4.207
VAL694
3.591
PHE698
3.692
VAL780
4.496
PHE783
3.173
MET784
3.725
VAL787
3.641
VAL788
3.662
LEU791
4.023
LEU792
4.166
TRP864
2.974
VAL904
3.678
LEU907
3.622
PHE910
3.986
VAL912
4.810
ASN913
2.470
LYS914
4.606
|
|||||
| PDB ID: 6BWI 3.7 angstrom cryoEM structure of full length human TRPM4 | ||||||
| Method | Electron microscopy | Resolution | 3.70 Å | Mutation | No | [2] |
| PDB Sequence |
.
|
|||||
|
|
TRP680
2.389
ILE690
3.789
VAL694
3.624
VAL780
4.763
PHE783
3.044
MET784
3.955
VAL787
3.862
VAL788
3.216
LEU791
3.946
LEU792
4.018
SER863
3.190
TRP864
2.219
CYS867
3.399
ILE897
3.969
MET900
2.865
VAL901
3.368
VAL904
3.550
LEU907
4.006
PHE910
4.155
THR911
4.811
VAL912
4.342
ASN913
2.829
ILE920
3.535
VAL921
4.841
VAL923
4.431
SER924
2.834
LYS925
4.556
MET927
4.251
PHE931
3.858
LEU934
3.363
PHE936
3.893
TRP940
4.891
LEU941
4.802
PRO960
3.156
SER961
4.670
LEU963
2.815
ARG964
3.391
PHE967
3.045
TYR968
4.247
GLN1007
3.884
TYR1015
1.936
VAL1025
3.792
ILE1026
2.407
LEU1029
2.480
VAL1030
4.198
LEU1034
3.237
|
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| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Structure of the human TRPM4 ion channel in a lipid nanodisc. Science. 2018 Jan 12;359(6372):228-232. | ||||
| REF 2 | Structure of full-length human TRPM4. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2377-2382. | ||||
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