Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T65197 Target Info
Target Name Erythropoietin Receptor (EPOR)
Synonyms Full-length form; EPO-R
Target Type Successful Target
Gene Name EPOR
Biochemical Class Cytokine receptor
UniProt ID
EPOR_HUMAN 
Target Interacting Proteins  Top
Adapter proteins [+] 1 Adapter proteins +
Interacting Protein Name Growth factor receptor-bound protein 2 (GRB2) Interacting Protein Interacting Protein Info
UniProt ID
GRB2_HUMAN 
Synonyms SH2/SH3 adapter GRB2; Protein Ash; Growth factor receptor-bound protein 2; ASH; Adapter protein GRB2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Reconstituted Complex [2]
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Target Name Protein-tyrosine phosphatase SHP-2 (PTPN11) Clinical trial Target Interacting Protein Info
UniProt ID
PTN11_HUMAN 
Synonyms Tyrosine-protein phosphatase non-receptor type 11; SH-PTP3; SH-PTP2; SHPTP2; SHP-2; SHP2; PTP-2C; PTP2C; PTP-1D; Protein-tyrosine phosphatase SHP2; Protein-tyrosine phosphatase 2C; Protein-tyrosine phosphatase 1D
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Reconstituted Complex [4]
Kinases [+] 2 Kinases +
Interacting Protein Name Phosphatidylinositol 3-kinase regulatory subunit alpha (PIK3R1) Interacting Protein Interacting Protein Info
UniProt ID
P85A_HUMAN 
Synonyms AGM7; GRB1; IMD36; p85; p85.ALPHA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [5]
2 Affinity Capture-Western [6]
3 Protein-Fragment Complementation Assay [7]
Interacting Target Name Janus kinase 2 (JAK-2) Successful Target Interacting Protein Info
UniProt ID
JAK2_HUMAN 
Synonyms Tyrosine-protein kinase JAK2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Reconstituted Complex [9]
Transcription regulators [+] 1 Transcription regulators +
Interacting Protein Name Signal transducer and activator of transcription 5A (STA5A) Interacting Protein Interacting Protein Info
UniProt ID
STA5A_HUMAN 
Synonyms MGF; STAT5
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Protein-Fragment Complementation Assay [7]
3 Reconstituted Complex [10]
4 Reconstituted Complex [11]
Other proteins [+] 4 Other proteins +
Interacting Protein Name Suppressor of cytokine signaling 2 (SOCS2) Interacting Protein Interacting Protein Info
UniProt ID
SOCS2_HUMAN 
Synonyms CIS2; Cish2; SOCS.2; SSI.2; SSI2; STATI2
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Protein-Fragment Complementation Assay [13]
3 Protein-Fragment Complementation Assay [7]
4 Reconstituted Complex [13]
5 Yeast two hybrid [12]
Interacting Target Name Erythropoietin (EPO) Clinical trial Target Interacting Protein Info
UniProt ID
EPO_HUMAN 
Synonyms Epoetin
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [15]
3 Reconstituted Complex [16]
4 Yeast two hybrid [17]
Interacting Protein Name Cytokine-inducible SH2-containing protein (CISH) Interacting Protein Interacting Protein Info
UniProt ID
CISH_HUMAN 
Synonyms BACTS2; CIS; CIS.1; G18; SOCS
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [18]
2 Protein-Fragment Complementation Assay [13]
3 Protein-Fragment Complementation Assay [7]
4 Reconstituted Complex [13]
Interacting Target Name Suppressor of cytokine signaling 3 (SOCS3) Literature-reported Target Interacting Protein Info
UniProt ID
SOCS3_HUMAN 
Synonyms STAT-induced STAT inhibitor 3; STAT induced STAT inhibitor 3; SSI-3; SSI3; SOCS-3; Cytokine-inducible SH2 protein 3; CIS-3; CIS3
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [19]
2 Reconstituted Complex [20]
References  Top
REF 1 Erythropoietin and interleukin-3 activate tyrosine phosphorylation of CBL and association with CRK adaptor proteins. Blood. 1997 May 1;89(9):3166-74.
REF 2 Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway. J Biol Chem. 1996 May 24;271(21):12129-32.
REF 3 Tyrosine 425 within the activated erythropoietin receptor binds Syp, reduces the erythropoietin required for Syp tyrosine phosphorylation, and promotes mitogenesis. Blood. 1996 Jun 1;87(11):4495-501.
REF 4 Involvement of SH2-containing phosphotyrosine phosphatase Syp in erythropoietin receptor signal transduction pathways. J Biol Chem. 1995 Mar 10;270(10):5631-5.
REF 5 Role of the vav proto-oncogene product (Vav) in erythropoietin-mediated cell proliferation and phosphatidylinositol 3-kinase activity. J Biol Chem. 1997 May 30;272(22):14334-40.
REF 6 Phosphorylation of tyrosine 503 in the erythropoietin receptor (EpR) is essential for binding the P85 subunit of phosphatidylinositol (PI) 3-kinase and for EpR-associated PI 3-kinase activity. J Biol Chem. 1995 Oct 6;270(40):23402-8.
REF 7 A systematic scan of interactions with tyrosine motifs in the erythropoietin receptor using a mammalian 2-hybrid approach. Blood. 2005 Jun 1;105(11):4264-71.
REF 8 Tyrosine kinase receptor RON functions downstream of the erythropoietin receptor to induce expansion of erythroid progenitors. Blood. 2004 Jun 15;103(12):4457-65.
REF 9 The N-terminal domain of Janus kinase 2 is required for Golgi processing and cell surface expression of erythropoietin receptor. Mol Cell. 2001 Dec;8(6):1327-38.
REF 10 Physical and functional interactions between Stat5 and the tyrosine-phosphorylated receptors for erythropoietin and interleukin-3. Blood. 1996 Dec 15;88(12):4415-25.
REF 11 APS, an adaptor protein containing Pleckstrin homology (PH) and Src homology-2 (SH2) domains inhibits the JAK-STAT pathway in collaboration with c-Cbl. Leukemia. 1999 May;13(5):760-7.
REF 12 Design and application of a cytokine-receptor-based interaction trap. Nat Cell Biol. 2001 Dec;3(12):1114-9.
REF 13 The C-terminus of CIS defines its interaction pattern. Biochem J. 2007 Jan 1;401(1):257-67.
REF 14 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 15 beta-Trcp mediates ubiquitination and degradation of the erythropoietin receptor and controls cell proliferation. Blood. 2007 Jun 15;109(12):5215-22.
REF 16 Shared and unique determinants of the erythropoietin (EPO) receptor are important for binding EPO and EPO mimetic peptide. J Biol Chem. 1999 May 14;274(20):14163-9.
REF 17 Both proteasomes and lysosomes degrade the activated erythropoietin receptor. Blood. 2005 Jan 15;105(2):600-8.
REF 18 Phosphorylated CIS suppresses the Epo or JAK2 V617F mutant-triggered cell proliferation through binding to EpoR. Cell Signal. 2017 Feb;31:41-57.
REF 19 CIS3/SOCS-3 suppresses erythropoietin (EPO) signaling by binding the EPO receptor and JAK2. J Biol Chem. 2000 Sep 22;275(38):29338-47.
REF 20 A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor. Eur J Biochem. 2002 May;269(10):2516-26.

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