Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T82665 Target Info
Target Name Protein arginine methyltransferase 5 (PRMT5)
Synonyms Shk1 kinase-binding protein 1 homolog; SKB1Hs; SKB1 homolog; SKB1; Protein arginine N-methyltransferase 5; Jak-binding protein 1; JBP1; IBP72; Histone-arginine N-methyltransferase PRMT5; HRMT1L5; 72 kDa ICln-binding protein
Target Type Clinical trial Target
Gene Name PRMT5
Biochemical Class Methyltransferase
UniProt ID
ANM5_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Target Name Mitotic growth and transcription activator (BAF190A) Literature-reported Target Interacting Protein Info
UniProt ID
SMCA4_HUMAN 
Synonyms Transcription activator BRG1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SNF2L4; SNF2-beta; SNF2B; Protein BRG-1; Protein brahma homolog 1; BRG1-associated factor 190A; BRG1; ATP-dependent helicase SMARCA4
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Reconstituted Complex [1]
Alpha-type channels [+] 1 Alpha-type channels +
Interacting Protein Name Methylosome subunit pICln (CLNS1A) Interacting Protein Interacting Protein Info
UniProt ID
ICLN_HUMAN 
Synonyms CLCI; CLNS1B; ICln
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Reconstituted Complex [3]
6 Reconstituted Complex [5]
7 Yeast two hybrid [6]
8 Yeast two hybrid [7]
Kinases [+] 1 Kinases +
Interacting Target Name Janus kinase 2 (JAK-2) Successful Target Interacting Protein Info
UniProt ID
JAK2_HUMAN 
Synonyms Tyrosine-protein kinase JAK2
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Biochemical Activity [8]
3 Reconstituted Complex [9]
4 Yeast two hybrid [9]
Other proteins [+] 1 Other proteins +
Interacting Protein Name Methylosome protein 50 (WDR77) Interacting Protein Interacting Protein Info
UniProt ID
MEP50_HUMAN 
Synonyms MEP.50; MEP50; Nbla10071; p44; p44/Mep50
Evidence Score (E-score) 13 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Affinity Capture-Western [2]
6 Affinity Capture-Western [10]
7 Affinity Capture-Western [11]
8 Co-crystal Structure [12]
9 Co-fractionation [13]
10 Co-purification [14]
11 Reconstituted Complex [3]
12 Yeast two hybrid [7]
13 Yeast two hybrid [15]
References  Top
REF 1 mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad. Mol Cell Biol. 2003 Nov;23(21):7475-87.
REF 2 Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase. Cancer Cell. 2010 Oct 19;18(4):329-40.
REF 3 A novel WD repeat protein component of the methylosome binds Sm proteins. J Biol Chem. 2002 Mar 8;277(10):8243-7.
REF 4 RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. J Biol Chem. 2011 Jan 21;286(3):1976-86.
REF 5 Role of pICLn in methylation of Sm proteins by PRMT5. J Biol Chem. 2009 Aug 7;284(32):21347-59.
REF 6 pICln binds to a mammalian homolog of a yeast protein involved in regulation of cell morphology. J Biol Chem. 1998 May 1;273(18):10811-4.
REF 7 Dynamic protein-protein interaction wiring of the human spliceosome. Mol Cell. 2012 Feb 24;45(4):567-80.
REF 8 JAK2V617F-mediated phosphorylation of PRMT5 downregulates its methyltransferase activity and promotes myeloproliferation. Cancer Cell. 2011 Feb 15;19(2):283-94.
REF 9 The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity. J Biol Chem. 1999 Oct 29;274(44):31531-42.
REF 10 Epigenetic mechanisms of Groucho/Grg/TLE mediated transcriptional repression. Mol Cell. 2012 Jan 27;45(2):185-95.
REF 11 The PRMT5/WDR77 complex regulates alternative splicing through ZNF326 in breast cancer. Nucleic Acids Res. 2017 Nov 2;45(19):11106-11120.
REF 12 Cryo-electron microscopy structure of a human PRMT5:MEP50 complex. PLoS One. 2018 Mar 8;13(3):e0193205.
REF 13 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 14 Crystal structure of the human PRMT5:MEP50 complex. Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17960-5.
REF 15 The histone-binding protein COPR5 is required for nuclear functions of the protein arginine methyltransferase PRMT5. EMBO Rep. 2008 May;9(5):452-8.

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