Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T96736 Target Info
Target Name Transforming protein RhoA (RHOA)
Synonyms h12; Rho cDNA clone 12; RHO12; ARHA; ARH12
Target Type Discontinued Target
Gene Name RHOA
Biochemical Class Small GTPase
UniProt ID
RHOA_HUMAN 
Target Interacting Proteins  Top
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Target Name Phospholipase D1 (PLD1) Patented-recorded Target Interacting Protein Info
UniProt ID
PLD1_HUMAN 
Synonyms PLD 1; Phosphatidylcholine-hydrolyzing phospholipase D1; HPLD1; Choline phosphatase 1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Biochemical Activity [2]
3 Protein-peptide [1]
4 Reconstituted Complex [2]
Kinases [+] 7 Kinases +
Interacting Protein Name Rho-associated protein kinase 1 (ROCK1) Interacting Protein Interacting Protein Info
UniProt ID
ROCK1_HUMAN 
Synonyms P160ROCK; ROCK.I
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Far Western [4]
3 Reconstituted Complex [3]
4 Reconstituted Complex [5]
5 Yeast two hybrid [3]
Interacting Protein Name Triple functional domain protein (TRIO) Interacting Protein Interacting Protein Info
UniProt ID
TRIO_HUMAN 
Synonyms ARHGEF23; tgat
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [6]
2 Co-fractionation [7]
3 Reconstituted Complex [6]
Interacting Target Name Rho-associated protein kinase 1 (ROCK1) Successful Target Interacting Protein Info
UniProt ID
ROCK1_HUMAN  
Synonyms Rok; ROCK1; ROCK; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated protein kinase 1; Rho-associated kinase 1; Rho kinase; P160ROCK; P160(rock); P160 ROCK-1; Let-502 kinase
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Far Western [4]
3 Reconstituted Complex [3]
4 Reconstituted Complex [5]
5 Yeast two hybrid [3]
Interacting Target Name MAPK/ERK kinase kinase 1 (MAP3K1) Clinical trial Target Interacting Protein Info
UniProt ID
M3K1_HUMAN 
Synonyms Mitogen-activated protein kinase kinase kinase 1; Mitogenactivated protein kinase kinase kinase 1; MEKK1; MEKK 1; MEKK; MEK kinase 1; MAPKKK1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Yeast two hybrid [8]
Interacting Target Name Protein kinase N1 (PKN1) Literature-reported Target Interacting Protein Info
UniProt ID
PKN1_HUMAN 
Synonyms Serine-threonine protein kinase N; Serine/threonine-protein kinase N1; Protein-kinase C-related kinase 1; Protein kinase PKN-alpha; Protein kinase C-like PKN; Protein kinase C-like 1; Protease-activated kinase 1; PRKCL1; PRK1; PKN; PAK1
Evidence Score (E-score) 2 Experimental Evidences +
1 Reconstituted Complex [3]
2 Yeast two hybrid [3]
Interacting Target Name Protein kinase N2 (PKN2) Literature-reported Target Interacting Protein Info
UniProt ID
PKN2_HUMAN 
Synonyms Serine/threonine-protein kinase N2; Protein-kinase C-related kinase 2; Protein kinase C-like 2; PRKCL2; PRK2; PKN gamma
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Reconstituted Complex [10]
Interacting Protein Name Citron Rho-interacting kinase (CIT) Interacting Protein Interacting Protein Info
UniProt ID
CTRO_HUMAN 
Synonyms CRIK; STK21
Evidence Score (E-score) 2 Experimental Evidences +
1 Reconstituted Complex [3]
2 Yeast two hybrid [3]
Transcription regulators [+] 1 Transcription regulators +
Interacting Protein Name Rho GTPase-activating protein 35 (ARHGAP35) Interacting Protein Interacting Protein Info
UniProt ID
RHG35_HUMAN 
Synonyms GRF.1; GRLF1; P190.A; P190A; p190ARhoGAP; p190RhoGAP
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [11]
2 Reconstituted Complex [12]
Other proteins [+] 7 Other proteins +
Interacting Protein Name Rho GDP-dissociation inhibitor 1 (ARHGDIA) Interacting Protein Interacting Protein Info
UniProt ID
GDIR1_HUMAN 
Synonyms GDIA1; HEL.S.47e; NPHS8; RHOGDI; RHOGDI.1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [13]
2 Co-crystal Structure [14]
3 Co-fractionation [14]
4 Reconstituted Complex [14]
5 Yeast two hybrid [15]
Interacting Target Name Apoptosis mediating surface antigen FAS (FAS) Clinical trial Target Interacting Protein Info
UniProt ID
TNR6_HUMAN 
Synonyms Tumor necrosis factor receptor superfamily member 6; TNFRSF6; FASLG receptor; FAS1; CD95; APT1; Apoptosis-mediating surface antigen FAS; Apo-1 antigen
Evidence Score (E-score) 2 Experimental Evidences +
1 Co-localization [16]
2 Co-purification [17]
Interacting Protein Name Rho guanine nucleotide exchange factor 2 (ARHGEF2) Interacting Protein Interacting Protein Info
UniProt ID
ARHG2_HUMAN 
Synonyms GEF; GEF.H1; GEFH1; LFP40; P40
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Affinity Capture-Western [18]
Interacting Protein Name Rho guanine nucleotide exchange factor 11 (ARHGEF11) Interacting Protein Interacting Protein Info
UniProt ID
ARHGB_HUMAN 
Synonyms GTRAP48; PDZ.RHOGEF
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Reconstituted Complex [19]
Interacting Protein Name Rho guanine nucleotide exchange factor 12 (ARHGEF12) Interacting Protein Interacting Protein Info
UniProt ID
ARHGC_HUMAN 
Synonyms LARG; PRO2792
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Reconstituted Complex [20]
Interacting Protein Name Disheveled-associated activator of morphogenesis 1 (DAAM1) Interacting Protein Interacting Protein Info
UniProt ID
DAAM1_HUMAN 
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Co-localization [16]
Interacting Protein Name Rho GTPase-activating protein 1 (ARHGAP1) Interacting Protein Interacting Protein Info
UniProt ID
RHG01_HUMAN 
Synonyms CDC42GAP; RHOGAP; RHOGAP1; p50rhoGAP
Evidence Score (E-score) 2 Experimental Evidences +
1 Reconstituted Complex [12]
2 Yeast two hybrid [21]
References  Top
REF 1 Determination of interaction sites of phospholipase D1 for RhoA. Biochem J. 2001 May 1;355(Pt 3):779-85.
REF 2 Activation of phospholipase D1 by ADP-ribosylated RhoA. Biochem Biophys Res Commun. 2003 Feb 28;302(1):127-32.
REF 3 RhoE binds to ROCK I and inhibits downstream signaling. Mol Cell Biol. 2003 Jun;23(12):4219-29.
REF 4 Identification of the Rho-binding domain of p160ROCK, a Rho-associated coiled-coil containing protein kinase. J Biol Chem. 1996 Sep 20;271(38):23022-8.
REF 5 The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol Cell Biol. 1996 Oct;16(10):5313-27.
REF 6 The trio guanine nucleotide exchange factor is a RhoA target. Binding of RhoA to the trio immunoglobulin-like domain. J Biol Chem. 2000 Nov 17;275(46):36116-23.
REF 7 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 8 RhoA binds to the amino terminus of MEKK1 and regulates its kinase activity. J Biol Chem. 2004 Jan 16;279(3):1872-7.
REF 9 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 10 Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling. J Biol Chem. 1996 Nov 15;271(46):28772-6.
REF 11 RhoA/ROCK activation by growth hormone abrogates p300/histone deacetylase 6 repression of Stat5-mediated transcription. J Biol Chem. 2004 Jul 30;279(31):32737-50.
REF 12 Regulation of RhoA GTP hydrolysis by the GTPase-activating proteins p190, p50RhoGAP, Bcr, and 3BP-1. Biochemistry. 1998 Apr 14;37(15):5249-57.
REF 13 Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding. J Cell Biol. 2001 Jan 8;152(1):111-26.
REF 14 Structural and Mechanistic Insights into the Regulation of the Fundamental Rho Regulator RhoGDI by Lysine Acetylation. J Biol Chem. 2016 Mar 11;291(11):5484-99.
REF 15 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 16 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 17 Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy. J Biol Chem. 2005 Mar 25;280(12):11641-7.
REF 18 Polarity-regulating kinase partitioning-defective 1b (PAR1b) phosphorylates guanine nucleotide exchange factor H1 (GEF-H1) to regulate RhoA-dependent actin cytoskeletal reorganization. J Biol Chem. 2011 Dec 30;286(52):44576-84.
REF 19 Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a dbl family member. FEBS Lett. 1999 Oct 15;459(3):313-8.
REF 20 Leukemia-associated Rho guanine nucleotide exchange factor, a Dbl family protein found mutated in leukemia, causes transformation by activation of RhoA. J Biol Chem. 2001 Jul 20;276(29):27145-51.
REF 21 Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways. Mol Cell Biol. 2004 Feb;24(4):1736-46.

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