Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T21307 Target Info
Target Name MAPK-activated protein kinase 2 (MAPKAPK2)
Synonyms MK2; MK-2; MAPKactivated protein kinase 2; MAPKAPK-2; MAPKAP-K2; MAPKAP kinase 2; MAP kinaseactivated protein kinase 2; MAP kinase-activated protein kinase 2
Target Type Clinical trial Target
Gene Name MAPKAPK2
Biochemical Class Kinase
UniProt ID
MAPK2_HUMAN 
Target Interacting Proteins  Top
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Protein Name Heat shock protein beta-1 (HSPB1) Interacting Protein Interacting Protein Info
UniProt ID
HSPB1_HUMAN 
Synonyms Stress-responsive protein 27; SRP27; HspB1; HSP28; HSP 27; Heat shock protein beta-1; Heat shock 27 kDa protein; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein
Evidence Score (E-score) 17 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Biochemical Activity [2]
3 Biochemical Activity [3]
4 Biochemical Activity [4]
5 Biochemical Activity [5]
6 Biochemical Activity [6]
7 Biochemical Activity [7]
8 Biochemical Activity [8]
9 Biochemical Activity [9]
10 Biochemical Activity [10]
11 Biochemical Activity [11]
12 Biochemical Activity [12]
13 Biochemical Activity [13]
14 Biochemical Activity [14]
15 Biochemical Activity [15]
16 Biochemical Activity [16]
17 Reconstituted Complex [1]
Kinases [+] 4 Kinases +
Interacting Target Name Stress-activated protein kinase 2a (p38 alpha) Clinical trial Target Interacting Protein Info
UniProt ID
MK14_HUMAN 
Synonyms SAPK2A; P38 Mitogen-activatedprotein kinase alpha; P38 mitogen activatedprotein kinase; MXI2; Mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase 14; MAX-interacting protein 2; MAPK 14; MAP kinase p38alpha; MAP kinase p38 alpha; MAP kinase MXI2; MAP kinase 14; Cytokine suppressive anti-inflammatory drug-binding protein; Cytokine suppressive anti-inflammatory drug binding protein; CSPB1; CSBP2; CSBP1; CSBP; CSAID-binding protein; CSAID binding protein; CRK1
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [17]
3 Co-crystal Structure [18]
4 Co-crystal Structure [19]
5 Protein-Fragment Complementation Assay [20]
6 Reconstituted Complex [1]
7 Reconstituted Complex [21]
Interacting Target Name RAC-alpha serine/threonine-protein kinase (AKT1) Successful Target Interacting Protein Info
UniProt ID
AKT1_HUMAN 
Synonyms RAC-PK-alpha; RAC; Proto-oncogene c-Akt; Protein kinase B alpha; Protein kinase B; PKB alpha; PKB
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Co-localization [22]
3 Reconstituted Complex [1]
Interacting Target Name Extracellular signal-regulated kinase 2 (ERK2) Clinical trial Target Interacting Protein Info
UniProt ID
MK01_HUMAN 
Synonyms PRKM2; PRKM1; P42-MAPK; P42 Mitogen-activated protein kinase; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; MAPK 2; MAPK 1; MAP kinase isoform p42; MAP kinase 2; MAP kinase 1; ERT1; ERK-2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Protein-Fragment Complementation Assay [20]
Interacting Target Name Stress-activated protein kinase 2b (p38 beta) Clinical trial Target Interacting Protein Info
UniProt ID
MK11_HUMAN 
Synonyms Stress-activated protein kinase-2; SAPK2b; SAPK2; PRKM11; P38b; P38-2; P38 Mitogen-activated protein kinase beta; Mitogen-activated protein kinase p38 beta; Mitogen-activated protein kinase 11; MAPK 11; MAP kinase p38 beta; MAP kinase 11
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [23]
2 Affinity Capture-Western [24]
Other proteins [+] 1 Other proteins +
Interacting Protein Name Polyhomeotic-like protein 2 (PHC2) Interacting Protein Interacting Protein Info
UniProt ID
PHC2_HUMAN 
Synonyms EDR2; HPH2; PH2
Evidence Score (E-score) 2 Experimental Evidences +
1 Protein-Fragment Complementation Assay [20]
2 Yeast two hybrid [25]
References  Top
REF 1 p38 Kinase-dependent MAPKAPK-2 activation functions as 3-phosphoinositide-dependent kinase-2 for Akt in human neutrophils. J Biol Chem. 2001 Feb 2;276(5):3517-23.
REF 2 Regulation of Hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor alpha by phosphorylation. J Biol Chem. 1999 Jul 2;274(27):18947-56.
REF 3 Heat shock protein 27 controls apoptosis by regulating Akt activation. J Biol Chem. 2003 Jul 25;278(30):27828-35.
REF 4 The p38 pathway provides negative feedback for Ras proliferative signaling. J Biol Chem. 2000 Dec 15;275(50):38973-80.
REF 5 Protein kinase C-delta (PKC-delta ) is activated by type I interferons and mediates phosphorylation of Stat1 on serine 727. J Biol Chem. 2002 Apr 26;277(17):14408-16.
REF 6 MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils. Cell Signal. 2003 Nov;15(11):993-1001.
REF 7 Tristetraprolin (TTP)-14-3-3 complex formation protects TTP from dephosphorylation by protein phosphatase 2a and stabilizes tumor necrosis factor-alpha mRNA. J Biol Chem. 2007 Feb 9;282(6):3766-77.
REF 8 Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2). J Biol Chem. 2002 Apr 26;277(17):14793-800.
REF 9 A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress. FEBS Lett. 1996 Sep 2;392(3):209-14.
REF 10 Chemotactic peptide N-formyl-met-leu-phe activation of p38 mitogen-activated protein kinase (MAPK) and MAPK-activated protein kinase-2 in human neutrophils. J Biol Chem. 1997 Jan 10;272(2):937-44.
REF 11 MAPKAP kinase 2 phosphorylates tristetraprolin on in vivo sites including Ser178, a site required for 14-3-3 binding. J Biol Chem. 2004 Mar 12;279(11):10176-84.
REF 12 MK2 SUMOylation regulates actin filament remodeling and subsequent migration in endothelial cells by inhibiting MK2 kinase and HSP27 phosphorylation. Blood. 2011 Feb 24;117(8):2527-37.
REF 13 The AU-rich element mRNA decay-promoting activity of BRF1 is regulated by mitogen-activated protein kinase-activated protein kinase 2. RNA. 2008 May;14(5):950-9.
REF 14 Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis. J Biol Chem. 2003 Sep 19;278(38):36410-7.
REF 15 The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ. Biochem J. 2005 Jul 1;389(Pt 1):127-35.
REF 16 p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun. 2015 Nov 30;6:10075.
REF 17 Identification of two distinct regions of p38 MAPK required for substrate binding and phosphorylation. Biochem Biophys Res Commun. 1999 Dec 9;266(1):284-9.
REF 18 Molecular basis of MAPK-activated protein kinase 2:p38 assembly. Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6353-8.
REF 19 Crystal structure of the p38 alpha-MAPKAP kinase 2 heterodimer. J Biol Chem. 2007 Mar 30;282(13):9733-9.
REF 20 Monitoring protein-protein interactions in mammalian cells by trans-SUMOylation. Biochem J. 2011 Sep 15;438(3):495-503.
REF 21 Creation of a stress-activated p90 ribosomal S6 kinase. The carboxyl-terminal tail of the MAPK-activated protein kinases dictates the signal transduction pathway in which they function. J Biol Chem. 2000 Oct 13;275(41):31588-93.
REF 22 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 23 The protein interaction landscape of the human CMGC kinase group. Cell Rep. 2013 Apr 25;3(4):1306-20.
REF 24 Cell type-specific inhibition of the ETS transcription factor ER81 by mitogen-activated protein kinase-activated protein kinase 2. J Biol Chem. 2001 Nov 9;276(45):41856-61.
REF 25 P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity. FEBS Lett. 2004 Apr 23;564(1-2):205-11.

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