Target Interacting Protein Information

Content Navigation (  All   None )                                      
Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T37961 Target Info
Target Name PAK-1 protein kinase (PAK1)
Synonyms p65-PAK; p21-activated kinase 1; Serine/threonine-protein kinase PAK 1; PAK-1; Alpha-PAK
Target Type Literature-reported Target
Gene Name PAK1
Biochemical Class Kinase
UniProt ID
PAK1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 2 Acid anhydride hydrolases +
Interacting Target Name Ras-related C3 botulinum toxin substrate 1 (RAC1) Literature-reported Target Interacting Protein Info
UniProt ID
RAC1_HUMAN 
Synonyms TC25; Ras-like protein TC25; p21-Rac1; MIG5; Cell migration-inducing gene5 protein; Cell migration-inducing gene 5 protein
Evidence Score (E-score) 17 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Affinity Capture-Western [5]
6 Affinity Capture-Western [6]
7 Affinity Capture-Western [7]
8 Affinity Capture-Western [8]
9 Affinity Capture-Western [9]
10 Affinity Capture-Western [10]
11 Reconstituted Complex [11]
12 Reconstituted Complex [12]
13 Reconstituted Complex [13]
14 Reconstituted Complex [14]
15 Reconstituted Complex [15]
16 Reconstituted Complex [16]
17 Reconstituted Complex [17]
Interacting Protein Name Cell division control protein 42 homolog (CDC42) Interacting Protein Interacting Protein Info
UniProt ID
CDC42_HUMAN 
Synonyms CDC42Hs; G25K
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [2]
3 Reconstituted Complex [13]
4 Reconstituted Complex [19]
5 Reconstituted Complex [12]
Adapter proteins [+] 2 Adapter proteins +
Interacting Protein Name Cytoplasmic protein NCK2 (NCK2) Interacting Protein Interacting Protein Info
UniProt ID
NCK2_HUMAN 
Synonyms GRB4; NCKbeta
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [20]
3 Yeast two hybrid [21]
Interacting Target Name NCK adaptor protein 1 (NCK1) Clinical trial Target Interacting Protein Info
UniProt ID
NCK1_HUMAN 
Synonyms SH2/SH3 adaptor protein NCK-alpha; SH2/SH3 adaptor protein NCKalpha; Nck-1; Nck1; NCK; Cytoplasmic protein NCK1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Reconstituted Complex [23]
Kinases [+] 1 Kinases +
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 2 Experimental Evidences +
1 Protein-Fragment Complementation Assay [24]
2 Yeast two hybrid [25]
Nuclear hormone receptors [+] 1 Nuclear hormone receptors +
Interacting Target Name Estrogen receptor (ESR) Successful Target Interacting Protein Info
UniProt ID
ESR1_HUMAN 
Synonyms Oestrogen receptor alpha; Oestrogen receptor; Nuclear receptor subfamily 3 group A member 1; NR3A1; Estrogen receptor alpha; Estradiol receptor; ESR; ER-alpha; ERalpha; ER
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [26]
2 Biochemical Activity [27]
3 Reconstituted Complex [27]
Other proteins [+] 1 Other proteins +
Interacting Protein Name Rho guanine nucleotide exchange factor 7 (ARHGEF7) Interacting Protein Interacting Protein Info
UniProt ID
ARHG7_HUMAN 
Synonyms BETA.PIX; COOL.1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [22]
3 Yeast two hybrid [28]
References  Top
REF 1 Integrin 7-mediated regulation of multiple myeloma cell adhesion, migration, and invasion. Blood. 2011 Jun 9;117(23):6202-13.
REF 2 A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells. Cell. 2006 May 5;125(3):535-48.
REF 3 A role for Mer tyrosine kinase in alphavbeta5 integrin-mediated phagocytosis of apoptotic cells. J Cell Sci. 2005 Feb 1;118(Pt 3):539-53.
REF 4 Isotype-specific degradation of Rac activated by the cytotoxic necrotizing factor 1. J Biol Chem. 2004 Aug 20;279(34):35840-8.
REF 5 Rab11-FIP3 is a Rab11-binding protein that regulates breast cancer cell motility by modulating the actin cytoskeleton. Eur J Cell Biol. 2009 Jun;88(6):325-41.
REF 6 Selective inhibition of Fcepsilon RI-mediated mast cell activation by a truncated variant of Cbl-b related to the rat model of type 1 diabetes mellitus. J Biochem. 2005 Jun;137(6):711-20.
REF 7 Rac1, but not Rac1B, stimulates RelB-mediated gene transcription in colorectal cancer cells. J Biol Chem. 2006 May 12;281(19):13724-32.
REF 8 Smad7 is required for TGF-beta-induced activation of the small GTPase Cdc42. J Cell Sci. 2004 Apr 1;117(Pt 9):1835-47.
REF 9 Protein kinase A-dependent phosphorylation of Dock180 at serine residue 1250 is important for glioma growth and invasion stimulated by platelet derived-growth factor receptor . Neuro Oncol. 2015 Jun;17(6):832-42.
REF 10 Ras, Rac1, and phosphatidylinositol-3-kinase (PI3K) signaling in nitric oxide induced endothelial cell migration. Nitric Oxide. 2015 May 1;47:40-51.
REF 11 RhoG activates Rac1 by direct interaction with the Dock180-binding protein Elmo. Nature. 2003 Jul 24;424(6947):461-4.
REF 12 Anosmin-1 modulates fibroblast growth factor receptor 1 signaling in human gonadotropin-releasing hormone olfactory neuroblasts through a heparan sulfate-dependent mechanism. J Neurosci. 2004 Nov 17;24(46):10384-92.
REF 13 ArhGAP15, a novel human RacGAP protein with GTPase binding property. FEBS Lett. 2003 Mar 27;539(1-3):131-7.
REF 14 The nuclear import of the small GTPase Rac1 is mediated by the direct interaction with karyopherin alpha2. Traffic. 2010 Feb;11(2):198-209.
REF 15 IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature. 2000 Dec 7;408(6813):732-5.
REF 16 Human Mena associates with Rac1 small GTPase in glioblastoma cell lines. PLoS One. 2009;4(3):e4765.
REF 17 Activation of the small GTPase Rac1 by cGMP-dependent protein kinase. Cell Signal. 2004 Sep;16(9):1061-9.
REF 18 Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS. Nat Methods. 2013 Apr;10(4):307-14.
REF 19 Cdc42 regulates E-cadherin ubiquitination and degradation through an epidermal growth factor receptor to Src-mediated pathway. J Biol Chem. 2008 Feb 22;283(8):5127-37.
REF 20 The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals. Nature. 2001 Sep 13;413(6852):174-9.
REF 21 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 22 A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT. EMBO J. 2001 Feb 1;20(3):457-65.
REF 23 Interaction of the Nck adapter protein with p21-activated kinase (PAK1). J Biol Chem. 1996 Oct 18;271(42):25746-9.
REF 24 The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nat Methods. 2014 May;11(5):585-92.
REF 25 In silico prediction of physical protein interactions and characterization of interactome orphans. Nat Methods. 2015 Jan;12(1):79-84.
REF 26 P21-activated kinase 1 regulation of estrogen receptor-alpha activation involves serine 305 activation linked with serine 118 phosphorylation. Cancer Res. 2006 Feb 1;66(3):1694-701.
REF 27 P21-activated kinase-1 phosphorylates and transactivates estrogen receptor-alpha and promotes hyperplasia in mammary epithelium. EMBO J. 2002 Oct 15;21(20):5437-47.
REF 28 A human MAP kinase interactome. Nat Methods. 2010 Oct;7(10):801-5.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.