Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T74066 Target Info
Target Name Polycomb complex protein BMI-1 (BMI1)
Synonyms RNF51; RING finger protein 51; Polycomb group RING finger protein 4; PCGF4
Target Type Clinical trial Target
Gene Name BMI1
Biochemical Class Zinc-finger
UniProt ID
BMI1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Target Name X-ray repair cross-complementing 5 (Ku80) Literature-reported Target Interacting Protein Info
UniProt ID
XRCC5_HUMAN 
Synonyms X-ray repair cross-complementing protein 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); TLAA; Thyroid-lupus autoantigen; Nuclear factor IV; Lupus Ku autoantigen protein p86; Ku86; G22P2; DNA repair protein XRCC5; CTCBF; CTC85; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; 86 kDa subunit of Ku antigen
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
Acyltransferases [+] 4 Acyltransferases +
Interacting Protein Name E3 ubiquitin-protein ligase RING2 (RNF2) Interacting Protein Interacting Protein Info
UniProt ID
RING2_HUMAN 
Synonyms BAP.1; BAP1; DING; HIPI3; RING1B; RING2
Evidence Score (E-score) 16 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
5 Affinity Capture-MS [6]
6 Affinity Capture-Western [7]
7 Affinity Capture-Western [4]
8 Affinity Capture-Western [8]
9 Affinity Capture-Western [9]
10 Affinity Capture-Western [10]
11 Affinity Capture-Western [11]
12 Co-crystal Structure [12]
13 Co-purification [13]
14 Co-purification [12]
15 Reconstituted Complex [12]
16 Reconstituted Complex [14]
Interacting Protein Name E3 ubiquitin-protein ligase RING1 (RING1) Interacting Protein Interacting Protein Info
UniProt ID
RING1_HUMAN 
Synonyms RING1A; RNF1
Evidence Score (E-score) 10 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
5 Affinity Capture-Western [15]
6 Affinity Capture-Western [10]
7 Affinity Capture-Western [16]
8 Co-purification [13]
9 Reconstituted Complex [17]
10 Yeast two hybrid [18]
Interacting Protein Name E3 SUMO-protein ligase CBX4 (CBX4) Interacting Protein Interacting Protein Info
UniProt ID
CBX4_HUMAN 
Synonyms NBP16; PC2
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
5 Affinity Capture-MS [6]
6 Affinity Capture-Western [15]
7 Affinity Capture-Western [19]
Interacting Protein Name Histone acetyltransferase KAT5 (KAT5) Interacting Protein Interacting Protein Info
UniProt ID
KAT5_HUMAN 
Synonyms ESA1; HTATIP; HTATIP1; PLIP; TIP; TIP60; ZC2HC5; cPLA2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [20]
2 Yeast two hybrid [21]
Histones [+] 4 Histones +
Interacting Target Name Gamma-Histone H2AX (H2AFX) Literature-reported Target Interacting Protein Info
UniProt ID
H2AX_HUMAN 
Synonyms Phosphorylation of H2AX; Histone H2AX; Histone H2A.x; H2AX; H2a/x
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Affinity Capture-Western [22]
3 Biochemical Activity [9]
4 Co-localization [23]
Interacting Protein Name Histone H2A type 2-C (HIST2H2AC) Interacting Protein Interacting Protein Info
UniProt ID
H2A2C_HUMAN 
Synonyms H2A; H2A.GL101; H2A/q; H2AFQ
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Biochemical Activity [12]
3 Biochemical Activity [24]
Interacting Protein Name Histone H1x (H1FX) Interacting Protein Interacting Protein Info
UniProt ID
H1X_HUMAN 
Synonyms H1.10; H1X
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Affinity Capture-MS [1]
Interacting Protein Name Histone H2A type 1-B/E (H2AFM/A) Interacting Protein Interacting Protein Info
UniProt ID
H2A1B_HUMAN 
Synonyms H2A/m; H2AFM
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Biochemical Activity [25]
Peptidases [+] 1 Peptidases +
Interacting Target Name Herpesvirus ubiquitin-specific protease (HAUSP) Preclinical Target Interacting Protein Info
UniProt ID
UBP7_HUMAN 
Synonyms Ubiquitin-specific-processing protease 7; Ubiquitin thioesterase 7; Ubiquitin carboxyl-terminal hydrolase 7; Herpesvirus-associated ubiquitin-specific protease; Deubiquitinating enzyme 7
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [26]
2 Reconstituted Complex [26]
Putative transporters [+] 1 Putative transporters +
Interacting Protein Name MICOS complex subunit MIC60 (IMMT) Interacting Protein Interacting Protein Info
UniProt ID
MIC60_HUMAN 
Synonyms HMP; MINOS2; P87; P87/89; P89; PIG52
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Yeast two hybrid [21]
Transcription regulators [+] 2 Transcription regulators +
Interacting Protein Name Chromobox protein homolog 2 (CBX2) Interacting Protein Interacting Protein Info
UniProt ID
CBX2_HUMAN 
Synonyms CDCA6; M33; SRXY5
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
5 Affinity Capture-Western [4]
Interacting Protein Name Zinc finger and BTB domain-containing protein 16 (ZBTB16) Interacting Protein Interacting Protein Info
UniProt ID
ZBT16_HUMAN 
Synonyms PLZF; ZNF145
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [27]
2 Affinity Capture-Western [17]
3 Co-fractionation [17]
4 Reconstituted Complex [27]
5 Reconstituted Complex [17]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Protein Name Ubiquitin-associated protein 2-like (UBAP2L) Interacting Protein Interacting Protein Info
UniProt ID
UBP2L_HUMAN 
Synonyms NICE.4
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [1]
Other proteins [+] 7 Other proteins +
Interacting Protein Name Polyhomeotic-like protein 2 (PHC2) Interacting Protein Interacting Protein Info
UniProt ID
PHC2_HUMAN 
Synonyms EDR2; HPH2; PH2
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
5 Affinity Capture-Western [28]
6 Co-crystal Structure [29]
7 Reconstituted Complex [29]
8 Yeast two hybrid [18]
9 Yeast two hybrid [30]
Interacting Protein Name Polyhomeotic-like protein 1 (PHC1) Interacting Protein Interacting Protein Info
UniProt ID
PHC1_HUMAN 
Synonyms EDR1; HPH1; MCPH11; RAE28
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [5]
4 Affinity Capture-MS [6]
5 Affinity Capture-Western [15]
6 Affinity Capture-Western [28]
7 Yeast two hybrid [31]
Interacting Protein Name Chromobox protein homolog 8 (CBX8) Interacting Protein Interacting Protein Info
UniProt ID
CBX8_HUMAN 
Synonyms PC3; RC1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
Interacting Protein Name Polyhomeotic-like protein 3 (PHC3) Interacting Protein Interacting Protein Info
UniProt ID
PHC3_HUMAN 
Synonyms EDR3; HPH3
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [1]
4 Affinity Capture-MS [5]
Interacting Protein Name RING1 and YY1-binding protein (RYBP) Interacting Protein Interacting Protein Info
UniProt ID
RYBP_HUMAN 
Synonyms AAP1; DEDAF; YEAF1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-MS [1]
3 Affinity Capture-MS [5]
4 Affinity Capture-Western [4]
Interacting Protein Name YY1-associated factor 2 (YAF2) Interacting Protein Interacting Protein Info
UniProt ID
YAF2_HUMAN 
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-MS [1]
3 Affinity Capture-MS [5]
4 Affinity Capture-Western [4]
Interacting Target Name Chromobox protein homolog 7 (CBX7) Literature-reported Target Interacting Protein Info
UniProt ID
CBX7_HUMAN 
Synonyms Chromobox 7
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-Western [3]
3 Reconstituted Complex [32]
References  Top
REF 1 The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127.
REF 2 BMI1 confers radioresistance to normal and cancerous neural stem cells through recruitment of the DNA damage response machinery. J Neurosci. 2010 Jul 28;30(30):10096-111.
REF 3 Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus. PLoS One. 2009 Jul 28;4(7):e6380.
REF 4 PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes. Mol Cell. 2012 Feb 10;45(3):344-56.
REF 5 A High-Density Map for Navigating the Human Polycomb Complexome. Cell Rep. 2016 Oct 4;17(2):583-595.
REF 6 BMI1-UBR5 axis regulates transcriptional repression at damaged chromatin. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11243-11248.
REF 7 The polycomb protein Ring1B generates self atypical mixed ubiquitin chains required for its in vitro histone H2A ligase activity. Mol Cell. 2006 Dec 8;24(5):701-711.
REF 8 The Polycomb Repressive Complex 1 Protein BMI1 Is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells. J Biol Chem. 2016 Jan 1;291(1):182-97.
REF 9 Monoubiquitination of H2AX protein regulates DNA damage response signaling. J Biol Chem. 2011 Aug 12;286(32):28599-607.
REF 10 Role of Bmi1 in H2A ubiquitylation and Hox gene silencing. J Biol Chem. 2006 Aug 11;281(32):22537-44.
REF 11 The Bmi-1 helix-turn and ring finger domains are required for Bmi-1 antagonism of (-) epigallocatechin-3-gallate suppression of skin cancer cell survival. Cell Signal. 2015 Jul;27(7):1336-44.
REF 12 Structure of a Bmi-1-Ring1B polycomb group ubiquitin ligase complex. J Biol Chem. 2006 Jul 21;281(29):20643-9.
REF 13 Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing. Mol Cell. 2005 Dec 22;20(6):845-54.
REF 14 BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase. Nat Commun. 2015 Jul 7;6:7621.
REF 15 RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor. Mol Cell Biol. 1997 Jul;17(7):4105-13.
REF 16 ASXL1 mutations promote myeloid transformation through loss of PRC2-mediated gene repression. Cancer Cell. 2012 Aug 14;22(2):180-93.
REF 17 The PRC1 Polycomb group complex interacts with PLZF/RARA to mediate leukemic transformation. Genes Dev. 2009 May 15;23(10):1195-206.
REF 18 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 19 Polycomb group protein-associated chromatin is reproduced in post-mitotic G1 phase and is required for S phase progression. J Biol Chem. 2008 Jul 4;283(27):18905-15.
REF 20 MDR1 mediated chemoresistance: BMI1 and TIP60 in action. Biochim Biophys Acta. 2016 Aug;1859(8):983-93.
REF 21 A human protein-protein interaction network: a resource for annotating the proteome. Cell. 2005 Sep 23;122(6):957-68.
REF 22 Radiosensitization of esophageal carcinoma cells by the silencing of BMI-1. Oncol Rep. 2016 Jun;35(6):3669-78.
REF 23 BMI1 is recruited to DNA breaks and contributes to DNA damage-induced H2A ubiquitination and repair. Mol Cell Biol. 2011 May;31(10):1972-82.
REF 24 Recognition of UbcH5c and the nucleosome by the Bmi1/Ring1b ubiquitin ligase complex. EMBO J. 2011 Jul 19;30(16):3285-97.
REF 25 In Vitro Assay to Study Histone Ubiquitination During Transcriptional Regulation. Methods Mol Biol. 2017;1507:235-244.
REF 26 Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. EMBO J. 2010 Aug 4;29(15):2553-65.
REF 27 Plzf mediates transcriptional repression of HoxD gene expression through chromatin remodeling. Dev Cell. 2002 Oct;3(4):499-510.
REF 28 Identification and characterization of interactions between the vertebrate polycomb-group protein BMI1 and human homologs of polyhomeotic. Mol Cell Biol. 1997 Apr;17(4):2326-35.
REF 29 BMI1 regulates PRC1 architecture and activity through homo- and hetero-oligomerization. Nat Commun. 2016 Nov 9;7:13343.
REF 30 Widespread macromolecular interaction perturbations in human genetic disorders. Cell. 2015 Apr 23;161(3):647-660.
REF 31 Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs. Structure. 2013 Apr 2;21(4):665-71.
REF 32 Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells. Mol Cell Proteomics. 2011 Apr;10(4):M110.002642.

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