Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T98459 Target Info
Target Name G2/mitotic-specific cyclin B1 (CCNB1)
Synonyms G2/mitotic-specific cyclin-B1; Cyclin B1; CCNB
Target Type Patented-recorded Target
Gene Name CCNB1
Biochemical Class Eukaryotic nuclear pore complex
UniProt ID
CCNB1_HUMAN 
Target Interacting Proteins  Top
Beta-transducin repeats [+] 2 Beta-transducin repeats +
Interacting Protein Name Fizzy-related protein homolog (FZR1) Interacting Protein Interacting Protein Info
UniProt ID
FZR1_HUMAN 
Synonyms CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1
Evidence Score (E-score) 3 Experimental Evidences +
1 Co-localization [1]
2 Reconstituted Complex [2]
3 Reconstituted Complex [3]
Interacting Protein Name Cell division cycle protein 20 homolog (CDC20) Interacting Protein Interacting Protein Info
UniProt ID
CDC20_HUMAN 
Synonyms CDC20A; bA276H19.3; p55CDC
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [4]
2 Reconstituted Complex [2]
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Target Name M-phase inducer phosphatase 1 (MPIP1) Literature-reported Target Interacting Protein Info
UniProt ID
MPIP1_HUMAN 
Synonyms Dual specificity phosphatase Cdc25A; Cdc25A phosphatase
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-Western [6]
Histones [+] 1 Histones +
Interacting Protein Name Histone H1.1 (HIST1H1A) Interacting Protein Interacting Protein Info
UniProt ID
H11_HUMAN 
Synonyms H1.1; H1A; H1F1; HIST1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Biochemical Activity [8]
3 Biochemical Activity [9]
4 Biochemical Activity [10]
5 Biochemical Activity [11]
Kinases [+] 2 Kinases +
Interacting Target Name Cyclin-dependent kinase 1 (CDK1) Clinical trial Target Interacting Protein Info
UniProt ID
CDK1_HUMAN 
Synonyms P34CDC2; P34 protein kinase; CDKN1; CDC28A; CDC2
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-MS [12]
2 Affinity Capture-MS [13]
3 Affinity Capture-Western [14]
4 Affinity Capture-Western [15]
5 Affinity Capture-Western [16]
6 Affinity Capture-Western [17]
7 Affinity Capture-Western [4]
8 Affinity Capture-Western [18]
9 Affinity Capture-Western [12]
10 Affinity Capture-Western [19]
11 Affinity Capture-Western [20]
12 Affinity Capture-Western [21]
13 Affinity Capture-Western [22]
14 Affinity Capture-Western [23]
15 Affinity Capture-Western [24]
16 Affinity Capture-Western [25]
17 Affinity Capture-Western [26]
18 Biochemical Activity [27]
19 Co-fractionation [28]
20 Co-purification [29]
Interacting Target Name Cyclin-dependent kinase 2 (CDK2) Clinical trial Target Interacting Protein Info
UniProt ID
CDK2_HUMAN 
Synonyms SIN3-associated protein; Sin3 associated polypeptide; P33 protein kinase; Cell division protein kinase 2; CDKN2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [30]
2 Reconstituted Complex [31]
Transcription regulators [+] 1 Transcription regulators +
Interacting Target Name Transcription factor Sp1 (SP1) Clinical trial Target Interacting Protein Info
UniProt ID
SP1_HUMAN 
Synonyms TSFP1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [32]
2 Co-localization [32]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Protein Name Cell division cycle protein 27 homolog (CDC27) Interacting Protein Interacting Protein Info
UniProt ID
CDC27_HUMAN 
Synonyms ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H.NUC; HNUC; NUC2
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [33]
2 Affinity Capture-Western [4]
3 Affinity Capture-Western [34]
4 Affinity Capture-Western [18]
5 Reconstituted Complex [3]
Other proteins [+] 3 Other proteins +
Interacting Target Name Melanoma differentiation-associated protein 6 (CDKN1A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1A_HUMAN 
Synonyms WAF1; SDI1; PIC1; P21(WAF1); P21; Melanoma differentiation associated protein 6; MDA-6; MDA6; Cyclin-dependent kinase inhibitor 1; CIP1; CDK-interacting protein 1; CAP20
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [35]
2 Affinity Capture-Western [15]
3 Co-localization [1]
4 Reconstituted Complex [36]
Interacting Protein Name Cyclin-dependent kinases regulatory subunit 1 (CKS1B) Interacting Protein Interacting Protein Info
UniProt ID
CKS1_HUMAN 
Synonyms CKS1; PNAS.16; PNAS.18; ckshs1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [13]
2 Affinity Capture-Western [37]
Interacting Protein Name Cyclin-dependent kinases regulatory subunit 2 (CKS2) Interacting Protein Interacting Protein Info
UniProt ID
CKS2_HUMAN 
Synonyms CKSHS2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [13]
2 Affinity Capture-Western [37]
References  Top
REF 1 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 2 Mitotic degradation of human thymidine kinase 1 is dependent on the anaphase-promoting complex/cyclosome-CDH1-mediated pathway. Mol Cell Biol. 2004 Jan;24(2):514-26.
REF 3 Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor. Genes Dev. 2006 Sep 1;20(17):2410-20.
REF 4 The APC/C recruits cyclin B1-Cdk1-Cks in prometaphase before D box recognition to control mitotic exit. J Cell Biol. 2010 Aug 23;190(4):587-602.
REF 5 Defining the Protein-Protein Interaction Network of the Human Protein Tyrosine Phosphatase Family. Mol Cell Proteomics. 2016 Sep;15(9):3030-44.
REF 6 Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins. Cell. 1991 Dec 20;67(6):1181-94.
REF 7 A p27Kip1 mutant that does not inhibit CDK activity promotes centrosome amplification and micronucleation. Oncogene. 2012 Aug 30;31(35):3989-98.
REF 8 Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 1999 May 6;18(18):2892-900.
REF 9 PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis. Mol Cell. 1998 Dec;2(6):877-85.
REF 10 Reconstitution of cyclin-dependent cdc2 and cdk2 kinase activities in vitro. J Biol Chem. 1993 Sep 25;268(27):20433-42.
REF 11 BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage. Nat Genet. 2002 Mar;30(3):285-9.
REF 12 Ubiquitin-specific protease 22 is a deubiquitinase of CCNB1. Cell Discov. 2015;1. pii: 15028.
REF 13 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 14 Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. Cell. 1989 Sep 8;58(5):833-46.
REF 15 APC/C(Cdc20) controls the ubiquitin-mediated degradation of p21 in prometaphase. Mol Cell. 2007 Aug 3;27(3):462-73.
REF 16 G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J Biol Chem. 1993 Apr 15;268(11):8298-308.
REF 17 Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage. J Biol Chem. 1996 May 31;271(22):13283-91.
REF 18 MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015 Mar 6;4(4):484-95.
REF 19 Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest. Mol Cell Biol. 1999 Feb;19(2):1460-9.
REF 20 The p53-targeting human phosphatase hCdc14A interacts with the Cdk1/cyclin B complex and is differentially expressed in human cancers. Mol Cancer. 2006 Jun 19;5:25.
REF 21 Depletion of Chk1 leads to premature activation of Cdc2-cyclin B and mitotic catastrophe. J Biol Chem. 2005 Nov 25;280(47):39246-52.
REF 22 Ubiquitin hydrolase Dub3 promotes oncogenic transformation by stabilizing Cdc25A. Nat Cell Biol. 2010 Apr;12(4):400-6.
REF 23 Human prostate epithelium lacks Wee1A-mediated DNA damage-induced checkpoint enforcement. Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7211-6.
REF 24 Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B. J Biol Chem. 2010 Dec 24;285(52):40544-53.
REF 25 HIV transcriptional activation by the accessory protein, VPR, is mediated by the p300 co-activator. Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5281-6.
REF 26 miR-192 induces G2/M growth arrest in aristolochic acid nephropathy. Am J Pathol. 2014 Apr;184(4):996-1009.
REF 27 A novel RING finger protein, human enhancer of invasion 10, alters mitotic progression through regulation of cyclin B levels. Mol Cell Biol. 2003 Mar;23(6):2109-22.
REF 28 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 29 Response gene to complement 32 is required for C5b-9 induced cell cycle activation in endothelial cells. Exp Mol Pathol. 2009 Apr;86(2):87-94.
REF 30 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 31 A nonproteolytic function of the proteasome is required for the dissociation of Cdc2 and cyclin B at the end of M phase. Genes Dev. 2000 Sep 15;14(18):2344-57.
REF 32 Sp1 phosphorylation by cyclin-dependent kinase 1/cyclin B1 represses its DNA-binding activity during mitosis in cancer cells. Oncogene. 2012 Nov 22;31(47):4946-59.
REF 33 Securin associates with APCCdh1 in prometaphase but its destruction is delayed by Rae1 and Nup98 until the metaphase/anaphase transition. Cell Cycle. 2006 Feb;5(4):366-70.
REF 34 Cyclin B and E2F-1 expression in prostate carcinoma cells treated with the novel retinoid CD437 are regulated by the ubiquitin-mediated pathway. Cancer Res. 2002 Jul 1;62(13):3842-9.
REF 35 LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells. Nat Commun. 2013;4:1531.
REF 36 p21WAF1/CIP1 regulates the p300 sumoylation motif CRD1 through a C-terminal domain independently of cyclin/CDK binding. Cell Cycle. 2005 Aug;4(8):1113-9.
REF 37 Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A. Mol Cell. 2008 May 9;30(3):290-302.

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