Target Interacting Protein Information

Content Navigation (  All   None )                                      
Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T53562 Target Info
Target Name X-ray repair cross-complementing 5 (Ku80)
Synonyms X-ray repair cross-complementing protein 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); Thyroid-lupus autoantigen; TLAA; Nuclear factor IV; Lupus Ku autoantigen protein p86; Ku86; G22P2; DNA repair protein XRCC5; CTCBF; CTC85; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; 86 kDa subunit of Ku antigen
Target Type Literature-reported Target
Gene Name XRCC5
Biochemical Class Acid anhydride hydrolase
UniProt ID
XRCC5_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 3 Acid anhydride hydrolases +
Interacting Protein Name X-ray repair cross-complementing protein 6 (XRCC6) Interacting Protein Interacting Protein Info
UniProt ID
XRCC6_HUMAN 
Synonyms CTC75; CTCBF; G22P1; KU70; ML8; TLAA
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [3]
4 Affinity Capture-Western [4]
5 Affinity Capture-Western [1]
6 Affinity Capture-Western [5]
7 Affinity Capture-Western [6]
8 Affinity Capture-Western [7]
9 Affinity Capture-Western [8]
10 Affinity Capture-Western [9]
11 Affinity Capture-Western [10]
12 Affinity Capture-Western [11]
13 Affinity Capture-Western [12]
14 Affinity Capture-Western [13]
15 Affinity Capture-Western [14]
16 Biochemical Activity [15]
17 Co-fractionation [1]
18 Co-fractionation [16]
19 Co-fractionation [17]
20 Co-localization [12]
Interacting Protein Name Werner syndrome ATP-dependent helicase (WRN) Interacting Protein Interacting Protein Info
UniProt ID
WRN_HUMAN 
Synonyms RECQ3; RECQL2; RECQL3
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-Western [6]
4 Affinity Capture-Western [1]
5 Affinity Capture-Western [18]
Interacting Protein Name ATP-dependent RNA helicase A (DHX9) Interacting Protein Interacting Protein Info
UniProt ID
DHX9_HUMAN 
Synonyms DDX9; LKP; NDH2; NDHII; RHA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [19]
2 Affinity Capture-Western [1]
3 Co-fractionation [19]
Acyltransferases [+] 1 Acyltransferases +
Interacting Protein Name Breast cancer type 1 susceptibility protein (BRCA1) Interacting Protein Interacting Protein Info
UniProt ID
BRCA1_HUMAN 
Synonyms BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [20]
2 Affinity Capture-Western [21]
3 Reconstituted Complex [20]
Isomerases [+] 1 Isomerases +
Interacting Target Name DNA topoisomerase I (TOP1) Successful Target Interacting Protein Info
UniProt ID
TOP1_HUMAN 
Synonyms DNA topoisomerase I
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [22]
Kinases [+] 2 Kinases +
Interacting Target Name DNA-dependent protein kinase catalytic (PRKDC) Clinical trial Target Interacting Protein Info
UniProt ID
PRKDC_HUMAN 
Synonyms P460; HYRC1; HYRC; DNPK1; DNA-PKcs; DNA-PK catalytic subunit; DNA-PK; DNA-dependent protein kinase catalytic subunit; DNA-dependent protein kinase
Evidence Score (E-score) 11 Experimental Evidences +
1 Affinity Capture-MS [2]
2 Affinity Capture-Western [23]
3 Affinity Capture-Western [24]
4 Affinity Capture-Western [25]
5 Affinity Capture-Western [1]
6 Affinity Capture-Western [5]
7 Biochemical Activity [15]
8 Co-fractionation [16]
9 Reconstituted Complex [26]
10 Reconstituted Complex [24]
11 Reconstituted Complex [27]
Interacting Target Name Telomerase reverse transcriptase (TERT) Clinical trial Target Interacting Protein Info
UniProt ID
TERT_HUMAN 
Synonyms TRT; TP2; Telomerase-associated protein 2; Telomerase catalytic subunit; TCS1; HEST2; EST2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [28]
2 Reconstituted Complex [28]
Transcription regulators [+] 2 Transcription regulators +
Interacting Target Name Telomeric repeat-binding factor 2 (TERF2) Literature-reported Target Interacting Protein Info
UniProt ID
TERF2_HUMAN 
Synonyms TTAGGG repeat-binding factor 2; TRF2; TRBF2; Telomeric DNA-binding protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [29]
2 Affinity Capture-Western [30]
Interacting Target Name Transcription factor 7-like 2 (TCF7L2) Literature-reported Target Interacting Protein Info
UniProt ID
TF7L2_HUMAN 
Synonyms TCF-4; TCF4; T-cell-specific transcription factor 4; T-cell transcription factor-4; T-cell factor 4; HTCF-4; HMG box transcription factor 4
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [31]
2 Affinity Capture-Western [32]
Other proteins [+] 4 Other proteins +
Interacting Target Name Poly [ADP-ribose] polymerase 1 (PARP1) Successful Target Interacting Protein Info
UniProt ID
PARP1_HUMAN 
Synonyms Protein poly-ADP-ribosyltransferase PARP1; PPOL; Poly[ADP-ribose] synthetase-1; Poly[ADP-ribose] synthase 1; Poly(ADP-ribose)polymerase-1; PARP-1; NAD(+)Poly [ADP-ribose] polymerase-1 ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase 1; DNA ADP-ribosyltransferase PARP1; ARTD1; ADPRT 1; ADPRT; ADP-ribosyltransferase diphtheria toxin-like 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [33]
3 Reconstituted Complex [34]
Interacting Target Name Proliferating cell nuclear antigen (PCNA) Clinical trial Target Interacting Protein Info
UniProt ID
PCNA_HUMAN 
Synonyms Cyclin
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [35]
2 Affinity Capture-Western [36]
Interacting Target Name Polycomb complex protein BMI-1 (BMI1) Clinical trial Target Interacting Protein Info
UniProt ID
BMI1_HUMAN 
Synonyms RNF51; RING finger protein 51; Polycomb group RING finger protein 4; PCGF4
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [37]
2 Affinity Capture-Western [38]
Interacting Protein Name N-terminal acetyltransferase (NAA15) Interacting Protein Interacting Protein Info
UniProt ID
NAA15_HUMAN 
Synonyms Ga19; NARG1; NAT1P; NATH; TBDN; TBDN100
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [2]
2 Affinity Capture-Western [4]
References  Top
REF 1 Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin. Mol Cancer. 2011 Jun 16;10:74.
REF 2 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 3 Ku autoantigen (DNA helicase) is required for interleukins-13/-4-induction of 15-lipoxygenase-1 gene expression in human epithelial cells. Genes Immun. 2000;1(4):237-50.
REF 4 Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex. J Biol Chem. 2002 Oct 4;277(40):37280-91.
REF 5 DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks. J Biol Chem. 2005 Feb 25;280(8):7060-9.
REF 6 Ku complex interacts with and stimulates the Werner protein. Genes Dev. 2000 Apr 15;14(8):907-12.
REF 7 The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell. 2010 Dec 22;40(6):976-87.
REF 8 Coilin interacts with Ku proteins and inhibits in vitro non-homologous DNA end joining. FEBS Lett. 2010 Dec 1;584(23):4735-9.
REF 9 The Ku protein complex interacts with YY1, is up-regulated in human heart failure, and represses alpha myosin heavy-chain gene expression. Mol Cell Biol. 2004 Oct;24(19):8705-15.
REF 10 Ku80 binds to human replication origins prior to the assembly of the ORC complex. Biochemistry. 2005 May 31;44(21):7885-96.
REF 11 Ku recruits the XRCC4-ligase IV complex to DNA ends. Mol Cell Biol. 2000 May;20(9):2996-3003.
REF 12 Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines. Breast Cancer Res. 2009;11(6):R83.
REF 13 Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2. Mol Biol Cell. 2017 Jan 1;28(1):192-200.
REF 14 FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4. Mol Cell. 2016 Feb 4;61(3):419-433.
REF 15 Association of the Ku autoantigen/DNA-dependent protein kinase holoenzyme and poly(ADP-ribose) polymerase with the DNA binding domain of progesterone receptors. J Mol Endocrinol. 2000 Apr;24(2):165-82.
REF 16 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 17 A high-throughput approach for measuring temporal changes in the interactome. Nat Methods. 2012 Sep;9(9):907-9.
REF 18 DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP. Mol Biol Cell. 2003 Oct;14(10):4221-9.
REF 19 DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner. Nucleic Acids Res. 2004 Jan 2;32(1):1-10.
REF 20 Rapid recruitment of BRCA1 to DNA double-strand breaks is dependent on its association with Ku80. Mol Cell Biol. 2008 Dec;28(24):7380-93.
REF 21 BRCA1-Ku80 protein interaction enhances end-joining fidelity of chromosomal double-strand breaks in the G1 phase of the cell cycle. J Biol Chem. 2013 Mar 29;288(13):8966-76.
REF 22 Camptothecin resistance is determined by the regulation of topoisomerase I degradation mediated by ubiquitin proteasome pathway. Oncotarget. 2017 Jul 4;8(27):43733-43751.
REF 23 Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication. Biochim Biophys Acta. 2002 Oct 11;1578(1-3):59-72.
REF 24 Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005 Mar 31;434(7033):605-11.
REF 25 Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends. J Biol Chem. 2006 Sep 22;281(38):27784-93.
REF 26 Binding of Ku and c-Abl at the kinase homology region of DNA-dependent protein kinase catalytic subunit. J Biol Chem. 1997 Oct 3;272(40):24763-6.
REF 27 Mapping of protein-protein interactions within the DNA-dependent protein kinase complex. Nucleic Acids Res. 1999 Sep 1;27(17):3494-502.
REF 28 Human Ku70/80 associates physically with telomerase through interaction with hTERT. J Biol Chem. 2002 Dec 6;277(49):47242-7.
REF 29 The protein network surrounding the human telomere repeat binding factors TRF1, TRF2, and POT1. PLoS One. 2010 Aug 25;5(8):e12407.
REF 30 The human Rap1 protein complex and modulation of telomere length. J Biol Chem. 2004 Jul 2;279(27):28585-91.
REF 31 Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in -Catenin-Active Cancers. Cell Syst. 2016 Sep 28;3(3):302-316.e4.
REF 32 Ku70 and poly(ADP-ribose) polymerase-1 competitively regulate beta-catenin and T-cell factor-4-mediated gene transactivation: possible linkage of DNA damage recognition and Wnt signaling. Cancer Res. 2007 Feb 1;67(3):911-8.
REF 33 Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences. J Biol Chem. 1999 Jul 16;274(29):20521-8.
REF 34 In prostate cancer cells the interaction of C/EBPalpha with Ku70, Ku80, and poly(ADP-ribose) polymerase-1 increases sensitivity to DNA damage. J Biol Chem. 2006 Apr 28;281(17):11496-505.
REF 35 A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein. J Biol Chem. 2002 Oct 25;277(43):40362-7.
REF 36 Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acids Res. 2001 Mar 15;29(6):1341-51.
REF 37 The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127.
REF 38 BMI1 confers radioresistance to normal and cancerous neural stem cells through recruitment of the DNA damage response machinery. J Neurosci. 2010 Jul 28;30(30):10096-111.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.