Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T81735 Target Info
Target Name DNA-dependent protein kinase catalytic (PRKDC)
Synonyms p460; HYRC1; HYRC; DNPK1; DNA-dependent protein kinase catalytic subunit; DNA-PKcs; DNA-PK catalytic subunit
Target Type Clinical trial Target
Gene Name PRKDC
Biochemical Class Kinase
UniProt ID
PRKDC_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 3 Acid anhydride hydrolases +
Interacting Target Name X-ray repair cross-complementing 5 (Ku80) Literature-reported Target Interacting Protein Info
UniProt ID
XRCC5_HUMAN 
Synonyms X-ray repair cross-complementing protein 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); TLAA; Thyroid-lupus autoantigen; Nuclear factor IV; Lupus Ku autoantigen protein p86; Ku86; G22P2; DNA repair protein XRCC5; CTCBF; CTC85; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; 86 kDa subunit of Ku antigen
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Biochemical Activity [5]
6 Co-crystal Structure [6]
7 Co-fractionation [7]
8 Co-localization [3]
9 Reconstituted Complex [8]
Interacting Protein Name X-ray repair cross-complementing protein 6 (XRCC6) Interacting Protein Interacting Protein Info
UniProt ID
XRCC6_HUMAN 
Synonyms CTC75; CTCBF; G22P1; KU70; ML8; TLAA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Affinity Capture-Western [10]
3 Biochemical Activity [5]
Interacting Protein Name Werner syndrome ATP-dependent helicase (WRN) Interacting Protein Interacting Protein Info
UniProt ID
WRN_HUMAN 
Synonyms RECQ3; RECQL2; RECQL3
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [11]
2 Protein-peptide [12]
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Protein Name Protein artemis (DCLRE1C) Interacting Protein Interacting Protein Info
UniProt ID
DCR1C_HUMAN 
Synonyms A.SCID; DCLREC1C; RS.SCID; SCIDA; SNM1C
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [4]
2 Biochemical Activity [13]
3 Biochemical Activity [14]
4 Reconstituted Complex [13]
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Target Name Lipopolysaccharide-associated protein 1 (HSPA8) Literature-reported Target Interacting Protein Info
UniProt ID
HSP7C_HUMAN 
Synonyms LPS-associated protein 1; LAP-1; HSPA10; HSP73; HSC70; Heat shock protein 73; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [15]
2 Co-fractionation [16]
Kinases [+] 2 Kinases +
Interacting Target Name RAC-alpha serine/threonine-protein kinase (AKT1) Successful Target Interacting Protein Info
UniProt ID
AKT1_HUMAN 
Synonyms RAC-PK-alpha; RAC; Proto-oncogene c-Akt; Protein kinase B alpha; Protein kinase B; PKB alpha; PKB
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [17]
2 Affinity Capture-Western [18]
3 Biochemical Activity [17]
4 Reconstituted Complex [18]
Interacting Target Name ATM serine/threonine kinase (ATM) Clinical trial Target Interacting Protein Info
UniProt ID
ATM_HUMAN 
Synonyms Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [19]
2 Protein-peptide [12]
Ligases [+] 1 Ligases +
Interacting Protein Name DNA ligase 4 (LIG4) Interacting Protein Interacting Protein Info
UniProt ID
DNLI4_HUMAN 
Synonyms LIG4S
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [20]
2 Protein-peptide [12]
Nuclear hormone receptors [+] 1 Nuclear hormone receptors +
Interacting Target Name Estrogen receptor (ESR) Successful Target Interacting Protein Info
UniProt ID
ESR1_HUMAN 
Synonyms Oestrogen receptor alpha; Oestrogen receptor; Nuclear receptor subfamily 3 group A member 1; NR3A1; Estrogen receptor alpha; Estradiol receptor; ESR; ER-alpha; ERalpha; ER
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [21]
2 Affinity Capture-Western [22]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-Western [23]
2 Biochemical Activity [24]
3 Biochemical Activity [12]
4 Biochemical Activity [25]
5 Biochemical Activity [26]
6 Biochemical Activity [27]
7 Biochemical Activity [28]
8 Biochemical Activity [29]
9 Protein-peptide [12]
Other proteins [+] 3 Other proteins +
Interacting Protein Name Replication protein A 32 kDa subunit (RPA2) Interacting Protein Interacting Protein Info
UniProt ID
RFA2_HUMAN 
Synonyms REPA2; RP.A p32; RP.A p34; RPA32
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Biochemical Activity [30]
3 Biochemical Activity [25]
4 Biochemical Activity [31]
5 Biochemical Activity [32]
6 Reconstituted Complex [9]
Interacting Protein Name Calcium and integrin-binding protein 1 (CIB1) Interacting Protein Interacting Protein Info
UniProt ID
CIB1_HUMAN 
Synonyms CIB; CIBP; KIP1; PRKDCIP; SIP2.28
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [33]
2 Yeast two hybrid [34]
Interacting Protein Name Interleukin enhancer-binding factor 3 (ILF3) Interacting Protein Interacting Protein Info
UniProt ID
ILF3_HUMAN 
Synonyms CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF.AT.90; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR.1; NFAR2; TCP110; TCP80
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Co-purification [35]
References  Top
REF 1 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 2 Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005 Mar 31;434(7033):605-11.
REF 3 Protein kinase CK2 localizes to sites of DNA double-strand break regulating the cellular response to DNA damage. BMC Mol Biol. 2012 Mar 9;13:7.
REF 4 Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends. J Biol Chem. 2006 Sep 22;281(38):27784-93.
REF 5 Association of the Ku autoantigen/DNA-dependent protein kinase holoenzyme and poly(ADP-ribose) polymerase with the DNA binding domain of progesterone receptors. J Mol Endocrinol. 2000 Apr;24(2):165-82.
REF 6 Crystal structure of DNA-PKcs reveals a large open-ring cradle comprised of HEAT repeats. Nature. 2010 Jan 7;463(7277):118-21.
REF 7 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 8 Binding of Ku and c-Abl at the kinase homology region of DNA-dependent protein kinase catalytic subunit. J Biol Chem. 1997 Oct 3;272(40):24763-6.
REF 9 Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999 Mar 1;18(5):1397-406.
REF 10 The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining. Mol Cell Biol. 2011 Dec;31(23):4832-43.
REF 11 Werner protein is a target of DNA-dependent protein kinase in vivo and in vitro, and its catalytic activities are regulated by phosphorylation. J Biol Chem. 2002 May 24;277(21):18291-302.
REF 12 Substrate specificities and identification of putative substrates of ATM kinase family members. J Biol Chem. 1999 Dec 31;274(53):37538-43.
REF 13 Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002 Mar 22;108(6):781-94.
REF 14 Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response. Mol Cell Biol. 2004 Oct;24(20):9207-20.
REF 15 Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in -Catenin-Active Cancers. Cell Syst. 2016 Sep 28;3(3):302-316.e4.
REF 16 A census of human soluble protein complexes. Cell. 2012 Aug 31;150(5):1068-81.
REF 17 Identification of a PKB/Akt hydrophobic motif Ser-473 kinase as DNA-dependent protein kinase. J Biol Chem. 2004 Sep 24;279(39):41189-96.
REF 18 DNA-PKcs, but not TLR9, is required for activation of Akt by CpG-DNA. EMBO J. 2005 Feb 23;24(4):779-89.
REF 19 Recruitment of ATM protein to double strand DNA irradiated with ionizing radiation. J Biol Chem. 1999 Sep 3;274(36):25571-5.
REF 20 Phosphorylation and regulation of DNA ligase IV stability by DNA-dependent protein kinase. J Biol Chem. 2004 Sep 3;279(36):37282-90.
REF 21 Identification of proteins associated with ligand-activated estrogen receptor in human breast cancer cell nuclei by tandem affinity purification and nano LC-MS/MS. Proteomics. 2011 Jan;11(1):172-9.
REF 22 A topoisomerase IIbeta-mediated dsDNA break required for regulated transcription. Science. 2006 Jun 23;312(5781):1798-802.
REF 23 Gene network analysis of oxidative stress-mediated drug sensitivity in resistant ovarian carcinoma cells. Pharmacogenomics J. 2010 Apr;10(2):94-104.
REF 24 Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex. J Biol Chem. 2003 Aug 8;278(32):29940-7.
REF 25 Purification and characterization of ATM from human placenta. A manganese-dependent, wortmannin-sensitive serine/threonine protein kinase. J Biol Chem. 2000 Mar 17;275(11):7803-10.
REF 26 DNA damage activates p53 through a phosphorylation-acetylation cascade. Genes Dev. 1998 Sep 15;12(18):2831-41.
REF 27 DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D. Genes Dev. 1998 Jul 15;12(14):2188-99.
REF 28 DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell. 1997 Oct 31;91(3):325-34.
REF 29 Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells. Nat Chem Biol. 2014 Dec;10(12):1000-2.
REF 30 Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to UV irradiation. Cancer Res. 2006 Mar 15;66(6):2997-3005.
REF 31 Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. J Biol Chem. 1997 Sep 19;272(38):23896-904.
REF 32 The DNA damage response in DNA-dependent protein kinase-deficient SCID mouse cells: replication protein A hyperphosphorylation and p53 induction. Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13825-30.
REF 33 DNA-protein kinase catalytic subunit-interacting protein KIP binds telomerase by interacting with human telomerase reverse transcriptase. J Biol Chem. 2004 Aug 13;279(33):34750-5.
REF 34 Interaction between DNA-dependent protein kinase and a novel protein, KIP. Mutat Res. 1997 Oct;385(1):13-20.
REF 35 DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45. J Biol Chem. 1998 Jan 23;273(4):2136-45.

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