Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T89361 Target Info
Target Name Cyclin-dependent kinase 6 (CDK6)
Synonyms Serine/threonine-protein kinase PLSTIRE; Serine/threonine protein kinase PLSTIRE; Cell division protein kinase 6; CDKN6
Target Type Successful Target
Gene Name CDK6
Biochemical Class Kinase
UniProt ID
CDK6_HUMAN 
Target Interacting Proteins  Top
Cell cycle related proteins [+] 7 Cell cycle related proteins +
Interacting Target Name Multiple tumor suppressor 1 (CDKN2A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN2A_HUMAN 
Synonyms Tumour suppressor gene p16; P16-INK4a; p16INK4A; P16-INK4; P16 gene; MTS-1; Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [3]
4 Affinity Capture-Western [4]
5 Biochemical Activity [5]
6 Co-crystal Structure [6]
7 Fluorescence Resonance Energy Transfer [7]
8 Protein-peptide [8]
9 Reconstituted Complex [9]
Interacting Target Name Cyclin D (CCND3) Literature-reported Target Interacting Protein Info
UniProt ID
CCND3_HUMAN 
Synonyms G1/S-specific cyclin-D3
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-Western [10]
3 Affinity Capture-Western [11]
4 Affinity Capture-Western [12]
5 Reconstituted Complex [13]
6 Yeast two hybrid [14]
7 Yeast two hybrid [15]
8 Yeast two hybrid [16]
Interacting Target Name CDK inhibitor 4C p18-INK4c (CDKN2C) Literature-reported Target Interacting Protein Info
UniProt ID
CDN2C_HUMAN 
Synonyms P18-INK6; P18-INK4c; P18(INK4c) protein; P18(INK4c); P18 INK4c protein; Cyclin-dependent kinase 6 inhibitor; Cyclin-dependent kinase 4 inhibitor C
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [2]
2 Affinity Capture-MS [3]
3 Affinity Capture-Western [17]
4 Co-crystal Structure [18]
5 Fluorescence Resonance Energy Transfer [7]
6 Reconstituted Complex [19]
7 Yeast two hybrid [20]
Interacting Protein Name Cyclin-dependent kinase 4 inhibitor B (CDKN2B) Interacting Protein Interacting Protein Info
UniProt ID
CDN2B_HUMAN 
Synonyms CDK4I; INK4B; MTS2; P15; TP15; p15INK4b
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [3]
4 Affinity Capture-Western [3]
5 Fluorescence Resonance Energy Transfer [3]
6 Yeast two hybrid [20]
Interacting Protein Name Cyclin-dependent kinase 4 inhibitor D (CDKN2D) Interacting Protein Interacting Protein Info
UniProt ID
CDN2D_HUMAN 
Synonyms INK4D; p19; p19.INK4D
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [3]
4 Co-crystal Structure [21]
5 Yeast two hybrid [20]
6 Yeast two hybrid [15]
Interacting Target Name G1/S-specific cyclin-D1 (CCND1) Clinical trial Target Interacting Protein Info
UniProt ID
CCND1_HUMAN 
Synonyms PRAD1 oncogene; PRAD1; Cyclin D1; BCL-1 oncogene; BCL-1; BCL1; B-cell lymphoma 1 protein
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Affinity Capture-Western [11]
3 Co-fractionation [22]
4 Reconstituted Complex [23]
Interacting Protein Name G1/S-specific cyclin-D2 (CCND2) Interacting Protein Interacting Protein Info
UniProt ID
CCND2_HUMAN 
Synonyms KIAK0002; MPPH3
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-Western [10]
3 Fluorescence Resonance Energy Transfer [7]
4 Yeast two hybrid [16]
Cell division cycle proteins [+] 1 Cell division cycle proteins +
Interacting Protein Name Retinoblastoma-associated protein (RB1) Interacting Protein Interacting Protein Info
UniProt ID
RB_HUMAN 
Synonyms OSRC; PPP1R130; RB; p105.Rb; pRb; pp110
Evidence Score (E-score) 8 Experimental Evidences +
1 Biochemical Activity [24]
2 Biochemical Activity [25]
3 Biochemical Activity [26]
4 Biochemical Activity [27]
5 Biochemical Activity [28]
6 Biochemical Activity [29]
7 Biochemical Activity [30]
8 Biochemical Activity [31]
Kinases [+] 1 Kinases +
Interacting Target Name Cyclin-dependent kinase 2 (CDK2) Clinical trial Target Interacting Protein Info
UniProt ID
CDK2_HUMAN 
Synonyms SIN3-associated protein; Sin3 associated polypeptide; P33 protein kinase; Cell division protein kinase 2; CDKN2
Evidence Score (E-score) 2 Experimental Evidences +
1 Co-localization [32]
2 Co-purification [33]
Other proteins [+] 2 Other proteins +
Interacting Target Name CDK inhibitor 1B p27Kip1 (CDKN1B) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1B_HUMAN 
Synonyms p27Kip1; KIP1; Cyclin-dependent kinase inhibitor p27; Cyclindependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [34]
3 Co-localization [32]
Interacting Target Name Melanoma differentiation-associated protein 6 (CDKN1A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1A_HUMAN 
Synonyms WAF1; SDI1; PIC1; P21(WAF1); P21; Melanoma differentiation associated protein 6; MDA-6; MDA6; Cyclin-dependent kinase inhibitor 1; CIP1; CDK-interacting protein 1; CAP20
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Reconstituted Complex [13]
References  Top
REF 1 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 2 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-440.
REF 3 The protein interaction landscape of the human CMGC kinase group. Cell Rep. 2013 Apr 25;3(4):1306-20.
REF 4 A CDKN2A mutation in familial melanoma that abrogates binding of p16INK4a to CDK4 but not CDK6. Cancer Res. 2007 Oct 1;67(19):9134-41.
REF 5 Hypo-phosphorylation of the retinoblastoma protein (pRb) by cyclin D:Cdk4/6 complexes results in active pRb. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10699-704.
REF 6 Structural basis for inhibition of the cyclin-dependent kinase Cdk6 by the tumour suppressor p16INK4a. Nature. 1998 Sep 17;395(6699):237-43.
REF 7 OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene. 2017 Oct 19;36(42):5852-5860.
REF 8 Inhibition of pRb phosphorylation and cell-cycle progression by a 20-residue peptide derived from p16CDKN2/INK4A. Curr Biol. 1996 Jan 1;6(1):84-91.
REF 9 CAK-independent activation of CDK6 by a viral cyclin. Mol Biol Cell. 2001 Dec;12(12):3987-99.
REF 10 Cdk6-cyclin D3 complex evades inhibition by inhibitor proteins and uniquely controls cell's proliferation competence. Oncogene. 2001 Apr 12;20(16):2000-9.
REF 11 Functional characterization of human PFTK1 as a cyclin-dependent kinase. Proc Natl Acad Sci U S A. 2007 May 29;104(22):9248-53.
REF 12 Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 1994 Mar;14(3):2077-86.
REF 13 Self-assembling protein microarrays. Science. 2004 Jul 2;305(5680):86-90.
REF 14 Next-generation sequencing to generate interactome datasets. Nat Methods. 2011 Jun;8(6):478-80.
REF 15 Pooled-matrix protein interaction screens using Barcode Fusion Genetics. Mol Syst Biol. 2016 Apr 22;12(4):863.
REF 16 rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions. Nat Commun. 2018 Sep 14;9(1):3747.
REF 17 Structure-based design of p18INK4c proteins with increased thermodynamic stability and cell cycle inhibitory activity. J Biol Chem. 2002 Dec 13;277(50):48827-33.
REF 18 Structural basis of inhibition of CDK-cyclin complexes by INK4 inhibitors. Genes Dev. 2000 Dec 15;14(24):3115-25.
REF 19 Growth suppression by p18, a p16INK4/MTS1- and p14INK4B/MTS2-related CDK6 inhibitor, correlates with wild-type pRb function. Genes Dev. 1994 Dec 15;8(24):2939-52.
REF 20 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 21 Crystal structure of the complex of the cyclin D-dependent kinase Cdk6 bound to the cell-cycle inhibitor p19INK4d. Nature. 1998 Sep 17;395(6699):244-50.
REF 22 CDK4 and CDK6 delay senescence by kinase-dependent and p16INK4a-independent mechanisms. Mol Cell Biol. 2007 Jun;27(12):4273-82.
REF 23 Regulation of CDK4 activity by a novel CDK4-binding protein, p34(SEI-1). Genes Dev. 1999 Nov 15;13(22):3027-33.
REF 24 Biologic and biochemical analyses of p16(INK4a) mutations from primary tumors. J Natl Cancer Inst. 1999 Sep 15;91(18):1569-74.
REF 25 PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis. Mol Cell. 1998 Dec;2(6):877-85.
REF 26 Direct interaction of p21 cyclin-dependent kinase inhibitor with the retinoblastoma tumor suppressor protein. Biochem Biophys Res Commun. 1999 Sep 16;263(1):35-40.
REF 27 Specific regulation of E2F family members by cyclin-dependent kinases. Mol Cell Biol. 1997 Jul;17(7):3867-75.
REF 28 Cyclin D3 is down-regulated by rapamycin in HER-2-overexpressing breast cancer cells. Mol Cancer Ther. 2006 Sep;5(9):2172-81.
REF 29 Both p16 and p21 families of cyclin-dependent kinase (CDK) inhibitors block the phosphorylation of cyclin-dependent kinases by the CDK-activating kinase. J Biol Chem. 1995 Aug 4;270(31):18195-7.
REF 30 p300 is required for orderly G1/S transition in human cancer cells. Oncogene. 2007 Jan 4;26(1):21-9.
REF 31 A systematic screen for CDK4/6 substrates links FOXM1 phosphorylation to senescence suppression in cancer cells. Cancer Cell. 2011 Nov 15;20(5):620-34.
REF 32 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 33 Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2C alpha and beta 2 isoforms. J Biol Chem. 2000 Nov 3;275(44):34744-9.
REF 34 S10 phosphorylation of p27 mediates atRA induced growth arrest in ovarian carcinoma cell lines. J Cell Physiol. 2008 Nov;217(2):558-68.

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