Target Interacting Protein Information
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T71081 | Target Info | |||
| Target Name | GTPase HRas (HRAS) | ||||
| Synonyms | p21ras; cHras; c-H-ras; Transforming protein p21; HaRas; Ha-Ras; H-Ras-1; GTPase HRas, Nterminally processed | ||||
| Target Type | Literature-reported Target | ||||
| Gene Name | HRAS | ||||
| Biochemical Class | Small GTPase | ||||
| UniProt ID | |||||
| Target Interacting Proteins | Top | ||||
|---|---|---|---|---|---|
| Kinases | [+] 2 Kinases | + | |||
| Interacting Target Name | Proto-oncogene c-RAF (c-RAF) | Clinical trial Target | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | Raf-1; RAF proto-oncogene serine/threonine-protein kinase; RAF; cRaf | ||||
| Evidence Score (E-score) | 3 Experimental Evidences | + | |||
| 1 | Affinity Capture-Western | [1] | |||
| 2 | Reconstituted Complex | [2] | |||
| 3 | Yeast two hybrid | [3] | |||
| Interacting Target Name | Serine/threonine-protein kinase B-raf (BRAF) | Successful Target | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | V-Raf murine sarcoma viral oncogene homolog B1; Serine/threonine-protein kinase B-raf; RAFB1; Proto-oncogene B-Raf; P94; BRAF1; BRAF(V599E); BRAF serine/threonine kinase; B-raf protein; B-Raf | ||||
| Evidence Score (E-score) | 2 Experimental Evidences | + | |||
| 1 | Reconstituted Complex | [4] | |||
| 2 | Yeast two hybrid | [3] | |||
| Other proteins | [+] 8 Other proteins | + | |||
| Interacting Target Name | GTPase activating protein (RASA1) | Literature-reported Target | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | RasGAP; RASA; Ras p21 protein activator; Ras GTPase-activating protein 1; p120GAP; GTPase-activating protein; GAP | ||||
| Evidence Score (E-score) | 4 Experimental Evidences | + | |||
| 1 | Affinity Capture-Western | [5] | |||
| 2 | Biochemical Activity | [6] | |||
| 3 | Co-crystal Structure | [7] | |||
| 4 | Reconstituted Complex | [8] | |||
| Interacting Protein Name | Afadin (AFDN) | Interacting Protein | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | AF6 | ||||
| Evidence Score (E-score) | 3 Experimental Evidences | + | |||
| 1 | Affinity Capture-Western | [9] | |||
| 2 | Reconstituted Complex | [9] | |||
| 3 | Yeast two hybrid | [10] | |||
| Interacting Protein Name | Ras and Rab interactor 1 (RIN1) | Interacting Protein | Interacting Protein Info | ||
| UniProt ID | |||||
| Evidence Score (E-score) | 3 Experimental Evidences | + | |||
| 1 | Affinity Capture-Western | [11] | |||
| 2 | Reconstituted Complex | [12] | |||
| 3 | Yeast two hybrid | [13] | |||
| Interacting Target Name | Galectin-1 (LGALS1) | Patented-recorded Target | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | S-Lac lectin 1; SLac lectin 1; Putative MAPK-activating protein PM12; Putative MAPKactivating protein PM12; Lectin galactoside-binding soluble 1; Lectin galactosidebinding soluble 1; Lactose-binding lectin 1; Lactosebinding lectin 1; HPL; HLBP14; HBL; Galaptin; Gal-1; Gal1; Beta-galactoside-binding lectin L-14-I; Betagalactosidebinding lectin L14I; 14 kDa lectin; 14 kDa laminin-binding protein; 14 kDa lamininbinding protein | ||||
| Evidence Score (E-score) | 2 Experimental Evidences | + | |||
| 1 | Affinity Capture-MS | [14] | |||
| 2 | Affinity Capture-Western | [14] | |||
| Interacting Protein Name | Ras association domain-containing protein 5 (RASSF5) | Interacting Protein | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | Maxp1; NORE1; NORE1A; NORE1B; RAPL; RASSF3 | ||||
| Evidence Score (E-score) | 2 Experimental Evidences | + | |||
| 1 | Reconstituted Complex | [15] | |||
| 2 | Yeast two hybrid | [16] | |||
| Interacting Protein Name | Son of sevenless homolog 1 (SOS1) | Interacting Protein | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | GF1; GGF1; GINGF; HGF; NS4 | ||||
| Evidence Score (E-score) | 2 Experimental Evidences | + | |||
| 1 | Affinity Capture-Western | [5] | |||
| 2 | Co-crystal Structure | [17] | |||
| Interacting Protein Name | Ral guanine nucleotide dissociation stimulator (RALGDS) | Interacting Protein | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | RGDS; RGF; RalGEF | ||||
| Evidence Score (E-score) | 1 Experimental Evidences | + | |||
| 1 | Yeast two hybrid | [13] | |||
| Interacting Protein Name | Ral guanine nucleotide dissociation stimulator-like 2 (RGL2) | Interacting Protein | Interacting Protein Info | ||
| UniProt ID | |||||
| Synonyms | HKE1.5; KE1.5; RAB2L | ||||
| Evidence Score (E-score) | 1 Experimental Evidences | + | |||
| 1 | Yeast two hybrid | [13] | |||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Rheb inhibits C-raf activity and B-raf/C-raf heterodimerization. J Biol Chem. 2006 Sep 1;281(35):25447-56. | ||||
| REF 2 | The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex with Ras and Raf. Genes Dev. 2000 Apr 15;14(8):895-900. | ||||
| REF 3 | Interaction of activated Ras with Raf-1 alone may be sufficient for transformation of rat2 cells. Mol Cell Biol. 1997 Jun;17(6):3047-55. | ||||
| REF 4 | Biochemical analysis of MEK activation in NIH3T3 fibroblasts. Identification of B-Raf and other activators. J Biol Chem. 1995 Mar 31;270(13):7644-55. | ||||
| REF 5 | EGFR phosphorylates FAM129B to promote Ras activation. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):644-9. | ||||
| REF 6 | Differential actions of p60c-Src and Lck kinases on the Ras regulators p120-GAP and GDP/GTP exchange factor CDC25Mm. Eur J Biochem. 2001 Jun;268(11):3275-83. | ||||
| REF 7 | The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants. Science. 1997 Jul 18;277(5324):333-8. | ||||
| REF 8 | Ras binding to a C-terminal region of GAP. FEBS Lett. 1995 Jul 17;368(2):297-303. | ||||
| REF 9 | In vivo interaction of AF-6 with activated Ras and ZO-1. Biochem Biophys Res Commun. 1999 May 27;259(1):103-7. | ||||
| REF 10 | The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9064-9. | ||||
| REF 11 | RIN1 is an ABL tyrosine kinase activator and a regulator of epithelial-cell adhesion and migration. Curr Biol. 2005 May 10;15(9):815-23. | ||||
| REF 12 | A human protein selected for interference with Ras function interacts directly with Ras and competes with Raf1. Mol Cell Biol. 1995 Mar;15(3):1318-23. | ||||
| REF 13 | Identification and characterization of rain, a novel Ras-interacting protein with a unique subcellular localization. J Biol Chem. 2004 May 21;279(21):22353-61. | ||||
| REF 14 | Galectin-1 binds oncogenic H-Ras to mediate Ras membrane anchorage and cell transformation. Oncogene. 2001 Nov 8;20(51):7486-93. | ||||
| REF 15 | Identification of Nore1 as a potential Ras effector. J Biol Chem. 1998 Mar 6;273(10):5439-42. | ||||
| REF 16 | Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism. Nat Commun. 2014 Apr 11;5:3650. | ||||
| REF 17 | The structural basis of the activation of Ras by Sos. Nature. 1998 Jul 23;394(6691):337-43. | ||||
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