Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T14731 Target Info
Target Name NAD-dependent deacetylase sirtuin-1 (SIRT1)
Synonyms hSIRT1; hSIR2; SIR2L1; SIR2-like protein 1; Regulatory protein SIR2 homolog 1; NAD-dependent protein deacetylase sirtuin-1
Target Type Clinical trial Target
Gene Name SIRT1
Biochemical Class Carbon-nitrogen hydrolase
UniProt ID
SIR1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Protein Name Werner syndrome ATP-dependent helicase (WRN) Interacting Protein Interacting Protein Info
UniProt ID
WRN_HUMAN 
Synonyms RECQ3; RECQL2; RECQL3
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [1]
3 Affinity Capture-Western [2]
4 Biochemical Activity [2]
Acyltransferases [+] 2 Acyltransferases +
Interacting Target Name Transcription intermediary factor 1-beta (TRIM28) Literature-reported Target Interacting Protein Info
UniProt ID
TIF1B_HUMAN 
Synonyms Tripartite motif-containing protein 28; TIF1-beta; TIF1B; RNF96; RING-type E3 ubiquitin transferase TIF1-beta; RING finger protein 96; Nuclear corepressor KAP-1; KRIP-1; KRAB-interacting protein 1; KRAB-associated protein 1; KAP-1; KAP1; E3 SUMO-protein ligase TRIM28
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Affinity Capture-Western [4]
3 Biochemical Activity [4]
Interacting Protein Name Histone acetyltransferase KAT5 (KAT5) Interacting Protein Interacting Protein Info
UniProt ID
KAT5_HUMAN 
Synonyms ESA1; HTATIP; HTATIP1; PLIP; TIP; TIP60; ZC2HC5; cPLA2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [5]
2 Biochemical Activity [6]
One-carbon transferases [+] 2 One-carbon transferases +
Interacting Target Name Enhancer of zeste homolog 2 (EZH2) Successful Target Interacting Protein Info
UniProt ID
EZH2_HUMAN 
Synonyms Lysine N-methyltransferase 6; Lysine Nmethyltransferase 6; KMT6; Histone-lysine N-methyltransferase EZH2; Histonelysine Nmethyltransferase EZH2; EZH2 Y641N mutations; EZH2 Y641F mutations; ENX-1; ENX1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Affinity Capture-Western [8]
3 Affinity Capture-Western [9]
4 Biochemical Activity [8]
Interacting Target Name DNA [cytosine-5]-methyltransferase 1 (DNMT1) Clinical trial Target Interacting Protein Info
UniProt ID
DNMT1_HUMAN 
Synonyms MCMT; M.HsaI; Dnmt1; DNMT; DNA MTase HsaI; DNA methyltransferase HsaI; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine5)methyltransferase 1; CXXC-type zinc finger protein 9; CXXCtype zinc finger protein 9; CXXC9; AIM
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Affinity Capture-Western [9]
3 Biochemical Activity [10]
Peptidases [+] 1 Peptidases +
Interacting Protein Name Ubiquitin carboxyl-terminal hydrolase 22 (USP22) Interacting Protein Interacting Protein Info
UniProt ID
UBP22_HUMAN 
Synonyms USP3L
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-Western [3]
3 Affinity Capture-Western [12]
4 Affinity Capture-Western [11]
5 Affinity Capture-Western [13]
6 Co-fractionation [11]
Pore-forming toxins [+] 2 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 12 Experimental Evidences +
1 Affinity Capture-Western [14]
2 Affinity Capture-Western [15]
3 Affinity Capture-Western [16]
4 Affinity Capture-Western [17]
5 Affinity Capture-Western [18]
6 Affinity Capture-Western [19]
7 Affinity Capture-Western [20]
8 Biochemical Activity [18]
9 Biochemical Activity [15]
10 Biochemical Activity [16]
11 Biochemical Activity [21]
12 Reconstituted Complex [21]
Interacting Protein Name BMP/retinoic acid-inducible neural-specific protein 1 (BRINP1) Interacting Protein Interacting Protein Info
UniProt ID
BRNP1_HUMAN 
Synonyms DBC1; DBCCR1; FAM5A
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-MS [16]
3 Affinity Capture-Western [14]
4 Affinity Capture-Western [16]
5 Affinity Capture-Western [22]
6 Reconstituted Complex [14]
Transcription regulators [+] 5 Transcription regulators +
Interacting Protein Name Forkhead box protein O3 (FOXO3) Interacting Protein Interacting Protein Info
UniProt ID
FOXO3_HUMAN 
Synonyms AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [23]
2 Affinity Capture-Western [24]
3 Affinity Capture-Western [25]
4 Affinity Capture-Western [26]
5 Biochemical Activity [26]
Interacting Target Name Transcription factor AP-1 (JUN) Discontinued Target Interacting Protein Info
UniProt ID
JUN_HUMAN 
Synonyms V-jun avian sarcoma virus 17 oncogene homolog; Transcription factor AP-1; Proto-oncogene c-jun; P39; C-jun proto-oncogene; AP-1 transcription factor; AP1; Activator protein-1; Activator protein 1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-Western [27]
3 Biochemical Activity [28]
4 Reconstituted Complex [28]
Interacting Target Name Sterol regulatory element binding protein-1 (SREBF1) Literature-reported Target Interacting Protein Info
UniProt ID
SRBP1_HUMAN 
Synonyms Sterol regulatory element-binding transcription factor 1; Sterol regulatory element-binding protein 1; SREBP-1; SREBP1; SREBP 1; Class D basic helix-loop-helix protein 1; bHLHd1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [29]
2 Biochemical Activity [30]
3 Biochemical Activity [29]
4 Reconstituted Complex [29]
Interacting Target Name Hypoxia-inducible factor 1 alpha (HIF-1A) Clinical trial Target Interacting Protein Info
UniProt ID
HIF1A_HUMAN 
Synonyms Transcription factor HIF-1; PASD8; PAS domain-containing protein 8; MOP1; Member of PAS protein 1; Hypoxia-inducible transcription factor (HIF)-1; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1; Hypoxia inducible factor 1; HIF-1-alpha; HIF-1alpha; HIF1-alpha; HIF-1 alpha; HIF1 alpha; Class E basic helix-loop-helix protein 78; bHLHe78; Basic-helix-loop-helix-PAS protein MOP1; ARNT-interacting protein; ARNT interacting protein
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [31]
2 Affinity Capture-Western [32]
3 Reconstituted Complex [32]
Interacting Target Name E2F transcription factor 1 (E2F1) Clinical trial Target Interacting Protein Info
UniProt ID
E2F1_HUMAN 
Synonyms Transcription factor E2F1; Retinoblastoma-binding protein 3; Retinoblastoma-associated protein 1; RBBP-3; RBBP3; RBAP-1; pRB-binding protein E2F-1; PBR3; E2F-1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [33]
2 Affinity Capture-Western [34]
3 Reconstituted Complex [34]
Other proteins [+] 3 Other proteins +
Interacting Protein Name Active regulator of SIRT1 (RPS19BP1) Interacting Protein Interacting Protein Info
UniProt ID
AROS_HUMAN 
Synonyms AROS; S19BP; dJ1104E15.4
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-Western [21]
3 Reconstituted Complex [21]
4 Yeast two hybrid [21]
Interacting Target Name MTOR complex 2 (RICTOR) Literature-reported Target Interacting Protein Info
UniProt ID
RICTR_HUMAN 
Synonyms Rapamycin-insensitive companion of mTOR; KIAA1999; hAVO3; AVO3 homolog
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [35]
2 Biochemical Activity [36]
Interacting Protein Name Insulin receptor substrate 2 (IRS2) Interacting Protein Interacting Protein Info
UniProt ID
IRS2_HUMAN 
Synonyms IRS.2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [37]
2 Biochemical Activity [38]
References  Top
REF 1 Identification and characterization of proteins interacting with SIRT1 and SIRT3: implications in the anti-aging and metabolic effects of sirtuins. Proteomics. 2009 May;9(9):2444-56.
REF 2 Regulation of WRN protein cellular localization and enzymatic activities by SIRT1-mediated deacetylation. J Biol Chem. 2008 Mar 21;283(12):7590-8.
REF 3 USP22 antagonizes p53 transcriptional activation by deubiquitinating Sirt1 to suppress cell apoptosis and is required for mouse embryonic development. Mol Cell. 2012 May 25;46(4):484-94.
REF 4 KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair. PLoS One. 2015 Apr 23;10(4):e0123935.
REF 5 Sirt1 physically interacts with Tip60 and negatively regulates Tip60-mediated acetylation of H2AX. Biochem Biophys Res Commun. 2009 Dec 25;390(4):1355-60.
REF 6 SIRT1 regulates autoacetylation and histone acetyltransferase activity of TIP60. J Biol Chem. 2010 Apr 9;285(15):11458-64.
REF 7 Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation. Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1859-64.
REF 8 PCAF-primed EZH2 acetylation regulates its stability and promotes lung adenocarcinoma progression. Nucleic Acids Res. 2015 Apr 20;43(7):3591-604.
REF 9 Oxidative damage targets complexes containing DNA methyltransferases, SIRT1, and polycomb members to promoter CpG Islands. Cancer Cell. 2011 Nov 15;20(5):606-19.
REF 10 SIRT1 deacetylates the DNA methyltransferase 1 (DNMT1) protein and alters its activities. Mol Cell Biol. 2011 Dec;31(23):4720-34.
REF 11 A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex. Mol Cell Biol. 2013 Apr;33(8):1487-502.
REF 12 Stabilization of histone demethylase PHF8 by USP7 promotes breast carcinogenesis. J Clin Invest. 2016 Jun 1;126(6):2205-20.
REF 13 LncRNA HULC triggers autophagy via stabilizing Sirt1 and attenuates the chemosensitivity of HCC cells. Oncogene. 2017 Jun 22;36(25):3528-3540.
REF 14 Negative regulation of the deacetylase SIRT1 by DBC1. Nature. 2008 Jan 31;451(7178):587-90.
REF 15 Necdin regulates p53 acetylation via Sirtuin1 to modulate DNA damage response in cortical neurons. J Neurosci. 2008 Aug 27;28(35):8772-84.
REF 16 DBC1 is a negative regulator of SIRT1. Nature. 2008 Jan 31;451(7178):583-6.
REF 17 Methyltransferase Set7/9 regulates p53 activity by interacting with Sirtuin 1 (SIRT1). Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1925-30.
REF 18 Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence. EMBO J. 2002 May 15;21(10):2383-96.
REF 19 Sirt-1 is required for the inhibition of apoptosis and inflammatory responses in human tenocytes. J Biol Chem. 2012 Jul 27;287(31):25770-81.
REF 20 DBC1 phosphorylation by ATM/ATR inhibits SIRT1 deacetylase in response to DNA damage. J Mol Cell Biol. 2012 Oct;4(5):294-303.
REF 21 Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity. Mol Cell. 2007 Oct 26;28(2):277-90.
REF 22 HDAC3 is negatively regulated by the nuclear protein DBC1. J Biol Chem. 2010 Dec 24;285(52):40830-7.
REF 23 FOXO4 is acetylated upon peroxide stress and deacetylated by the longevity protein hSir2(SIRT1). J Biol Chem. 2004 Jul 9;279(28):28873-9.
REF 24 Stimulation of Sirt1-regulated FoxO protein function by the ligand-bound vitamin D receptor. Mol Cell Biol. 2010 Oct;30(20):4890-900.
REF 25 Resveratrol promotes osteogenesis of human mesenchymal stem cells by upregulating RUNX2 gene expression via the SIRT1/FOXO3A axis. J Bone Miner Res. 2011 Oct;26(10):2552-63.
REF 26 Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 2004 Mar 26;303(5666):2011-5.
REF 27 Inhibition of transcriptional activity of c-JUN by SIRT1. Biochem Biophys Res Commun. 2008 Nov 28;376(4):793-6.
REF 28 SIRT1 suppresses activator protein-1 transcriptional activity and cyclooxygenase-2 expression in macrophages. J Biol Chem. 2010 Mar 5;285(10):7097-110.
REF 29 SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism. J Biol Chem. 2010 Oct 29;285(44):33959-70.
REF 30 Conserved role of SIRT1 orthologs in fasting-dependent inhibition of the lipid/cholesterol regulator SREBP. Genes Dev. 2010 Jul 1;24(13):1403-17.
REF 31 Inhibition of SIRT1 impairs the accumulation and transcriptional activity of HIF-1 protein under hypoxic conditions. PLoS One. 2012;7(3):e33433.
REF 32 Sirtuin 1 modulates cellular responses to hypoxia by deacetylating hypoxia-inducible factor 1alpha. Mol Cell. 2010 Jun 25;38(6):864-78.
REF 33 hSirT1-dependent regulation of the PCAF-E2F1-p73 apoptotic pathway in response to DNA damage. Mol Cell Biol. 2009 Apr;29(8):1989-98.
REF 34 Interactions between E2F1 and SirT1 regulate apoptotic response to DNA damage. Nat Cell Biol. 2006 Sep;8(9):1025-31.
REF 35 Hepatic FOXO1 Target Genes Are Co-regulated by Thyroid Hormone via RICTOR Protein Deacetylation and MTORC2-AKT Protein Inhibition. J Biol Chem. 2016 Jan 1;291(1):198-214.
REF 36 Multiple site acetylation of Rictor stimulates mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of Akt protein. J Biol Chem. 2012 Jan 2;287(1):581-8.
REF 37 Phosphoinositide 3-kinase as a novel functional target for the regulation of the insulin signaling pathway by SIRT1. Mol Cell Endocrinol. 2011 Mar 30;335(2):166-76.
REF 38 The direct involvement of SirT1 in insulin-induced insulin receptor substrate-2 tyrosine phosphorylation. J Biol Chem. 2007 Nov 23;282(47):34356-64.

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